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The map label for this gene is ypfI

Identifier: 218700932

GI number: 218700932

Start: 2710568

End: 2712583

Strand: Reverse

Name: ypfI

Synonym: ECIAI39_2613

Alternate gene names: 218700932

Gene position: 2712583-2710568 (Counterclockwise)

Preceding gene: 218700933

Following gene: 218700931

Centisome position: 52.86

GC content: 55.26

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTCACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGCGTTGGTGTTTTGATCATGCGCTTAAGTTGCGTGATGCCTTACCTGGCGACTGGCTGTGGATTTCGCCGCAGC
CAGATGCTGAAAACCACTGTTTTCCCTCGGCGCTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCACGGCTTTGATGCCGCTGCCTTTGCGGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTGGTGTTGTTACTCCC
TGTATGGGAAGAGTGGGAAAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCAGCATCTCAAACGCGTACTTACGGCGAATAACGACGCTATCCTCTGGCGGCAGAACCAGCCGTTCACGTTG
GCGCATTTTACTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAGCCAGAACAACAGCAACTCTTACAGCAGCT
ACTGACCATGCCATTGGGCGTGGCGGTGGTAACGGCTGCGCGTGGGCGCGGTAAATCGGCGCTGGCAGGGCAACTCATTT
CTCGTATTGCGGGTAGTGCGATTGTCACCGCGCCCGCAAAAGCGGCAACGTATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCGCCGGATGCCTTGTTAGCCAGCGATGAGCAAGCCGACTGGCTGGTGGTCGATGAAGCCGCAGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGCA
CGGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTTGAACTGCAACAGCCGATCCGCTGG
GCACAGGGATGCCCGCTGGAAAAAATGGTTAGTGAGGCACTGGTTTTTGACGATGAAAACTTCACCCACACACCACAAGG
CAATATCGTCATTTCCGCATTTGAACAGACGTTATGGCGAAGCGAGCCACAAACGCCGTTAAAGGTTTATCAATTATTGT
CTGGTGCGCACTACCGGACTTCGCCGCTGGATTTACGCCGCATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCT
GGCGAAAACGCGATTGCCGGGGCGCTGTGGCTGGTGGATGAGGGCGGATTATCTCAAGAACTCAGTCAGGCGGTATGGGC
AGGTTTTCGTCGCCCGCGGGGTAATCTGGTGGCCCAGTCGCTGGCGGCGCACGGCAGCAATCCACTGGCGGCGACATTGT
GCGGAAGGCGGGTTAGCCGGATAGCAGTTCATCCGGCTCGTCAGCGGGAAGGCACAGGTCAGCAACTGATTGCTGGTGCT
TTGCAATATACGCATGACCTCGACTATCTTTCGGTGAGTTTTGGTTACACCGGGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTGCGGATGGGTAATCATCGTGAAGCCAGCAGCGGTTGCTATACGGCGATGGCGCTGTTACCGATGA
GTGATGCGGGTAAACAACTGGCTGAAGGTGAGCATTACCGTTTACGTCGCGATGCGCAAGCTCTCGCAAAGTGGAATGGC
GAAACGCTTCCTGTTGATCCACTAAACGATGCCGTCCTTTCTGACGACGACTGGCTTGAACTGGCCGGTTTTGCTTTCGC
TCATCGTCCGCTATTAACATCGTTAGGTTGCTTATTGCGTCTGCTACAAACCAGTGAACTGGCATTACCGGCGCTGCGTG
GGCGTTTACAGAAAAACGTCAGCGACGCGCAGTTATGTACCACACTTAAACTTTCAGGCCGCAAGATGTTACTGGTCCGT
CAGCGGGAAGAGGCCGCGCAGGCGCTGTTCGCACTTAATGATGTTCGCACTGAGCGTCTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGTGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAACTTCCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALPGDWLWISPQPDAENHCFPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLCGRRVSRIAVHPARQREGTGQQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEGEHYRLRRDAQALAKWNG
ETLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNDVRTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALPGDWLWISPQPDAENHCFPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLCGRRVSRIAVHPARQREGTGQQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEGEHYRLRRDAQALAKWNG
ETLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNDVRTERLRDRITQWQFFH
>Mature_670_residues
AELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALPGDWLWISPQPDAENHCFPSALQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTLA
HFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAG
ENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLCGRRVSRIAVHPARQREGTGQQLIAGAL
QYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEGEHYRLRRDAQALAKWNGE
TLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQ
REEAAQALFALNDVRTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=95.6780923994039, Blast_Score=1306, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=366, Percent_Identity=27.0491803278689, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324197, Length=384, Percent_Identity=25.78125, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640662, Length=449, Percent_Identity=25.3897550111359, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74790; Mature: 74659

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALPGDWLWISPQPDAENHC
CCCHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
QQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
HHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENAIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLCGRRVSRIAVHPARQREGTGQQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEGEHYRLRRDAQALAKWNGETLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALPGDWLWISPQPDAENHC
CCHHHHHHHHHHHHHHHHHEEEHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
FPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
QQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATYVLAQFAGEK
HHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENAIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLCGRRVSRIAVHPARQREGTGQQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEGEHYRLRRDAQALAKWNGETLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]