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The map label for this gene is ypfH

Identifier: 218700931

GI number: 218700931

Start: 2709796

End: 2710494

Strand: Reverse

Name: ypfH

Synonym: ECIAI39_2612

Alternate gene names: 218700931

Gene position: 2710494-2709796 (Counterclockwise)

Preceding gene: 218700932

Following gene: 218700924

Centisome position: 52.81

GC content: 54.08

Gene sequence:

>699_bases
ATGAAACATGACCATTTTGTTGTTCAAAGCCCGGATAAACCTGCGCAACAGTTGTTACTGCTTTTCCACGGTGTCGGGGA
TAACCCGGTGGCAATGGGGGAAATAGGTAGCTGGTTTGCACCGCTATTTCCTGATGCGCTGGTGGTGAGTGTCGGAGGCG
CGGAGCCGAGCGGTAATCCGGCGGGGCGTCAGTGGTTTTCGGTGCAGGGTATTACGGAAGATAATCGCCAGGCGCGTGTG
GATGCCATCATGCCGACGTTTATTGAAACGGTGCGCTACTGGCAGAAACAGAGCGGAGTAGGGGCTAACGCCACGGCGCT
AATCGGTTTCTCTCAAGGCGCGATTATGGCGCTGGAGAGCATTAAAGCCGAACCGGGCCTTGCCTCACGCGTCATTGCCT
TTAACGGACGCTATGCGAGCCTGCCGGAAACTGCGTCTACAGCCACGACAATTCACCTGATTCATGGCGGTGAAGATCCG
GTTATCGATCTGGCGCATGCGGTTGCGGCGCAGGAAGCGTTAATCAGTGCCGGTGGTGACGTGACGCTGGATATCGTGGA
GGATTTAGGCCATGCGATCGACAATCGCAGTATGCAATTTGCTCTGGATCACTTGCGTTATACCATTCCGAAACATTATT
TCGACGAAGCGCTAAGCGGCGGTAAGCCTGGCGATGATGATGTGATTGAAATGATGTAA

Upstream 100 bases:

>100_bases
ATAACGCAATGGCAATTTTTTCACTGACTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGA
CATCAGTTAAGGAGTTCGCC

Downstream 100 bases:

>100_bases
TAATAGCCGGGTAGGTCTGATAAGACTGTATCAGGCATCATGGCCGGATGCGGCGTTAACGCCTTATCCGGCCACTTAGC
TTACTTCTTCGGTTGATCCT

Product: esterase YpfH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM

Sequences:

>Translated_232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
>Mature_232_residues
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARV
DAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM

Specific function: Displays esterase activity toward palmitoyl-CoA and pNP- butyrate [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI87082111, Length=232, Percent_Identity=99.1379310344828, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 24800; Mature: 24800

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNP
CCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEEEEECCCCCCCCC
AGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALES
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDPVIDLAHAVAAQEALISAGGD
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
EEEHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNP
CCCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEEEEECCCCCCCCC
AGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALES
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDPVIDLAHAVAAQEALISAGGD
HCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM
EEEHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]