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The map label for this gene is xapR

Identifier: 218700868

GI number: 218700868

Start: 2645054

End: 2645938

Strand: Reverse

Name: xapR

Synonym: ECIAI39_2547

Alternate gene names: 218700868

Gene position: 2645938-2645054 (Counterclockwise)

Preceding gene: 218700869

Following gene: 218700867

Centisome position: 51.56

GC content: 51.07

Gene sequence:

>885_bases
ATGGAAAGTACACATCGTATTGATCTCAAGTTATTGCGCTATTTTCTTGCGGTCGCAGAAGAACTTCACTTTGGGCGGGC
CGCTGCGCGGCTGAATATGTCGCAACCGCCATTAAGTATTCATATCAAAGATCTGGAACAACAGTTGGGCACTTTGCTGT
TCGTTCGGCATTCGCGCAGTGTGGCGCTCACTCATGCGGGGAAAATTTTACTGGAAGAGTCACGTCGTCTGCTGGCTAAT
GCTAGTCAGGCGTTGGCACGAGTAGAACAGATCGGTCGTGGTGAGGCAGGGCGCATTGAGCTGGGCGTGGTGGGAACCGC
AATGTGGGGGAGAATGCGTCCGGTAATGCGGCGGTTTTTGAAAGAGTGCCCTAATGTTCATGTCTCATTTCGTGAAAAAT
CACCAGGGATGCAAATGGCATTGCTCGAGCGGCGGGAGCTGGACGCGGGTATCTGGCGGATGGCAATTGAACCCACGACA
GGATTTACCAGTCTGCGATTACACGAATCTTCGTTTTTGGTGGCGATACCGGAAGATCATCATCTGGCTTGCGAAAGCGC
TATCCCGTTAATGGCATTACGCAATGAATACTTTGTGACGCTGCCATCGGTCCATTCCGATTGGGCGTTTTTACAGCGTG
TCTGTCAGCAGGCAGGGTTTTCACCGATGATCGTTCGGGAGGCCGTAGAACCGCAAACCGTACTGGCGATGATCAGTATG
GGGTTGGGTGTGACATTAATGGCAGATAGCTATGCCCAAATGAGCTGGCCGGGTGTGGTATTTCGTCCACTCGAGGAACG
TATTCCAGCAGATTTGTATATTGTTTACGAACAGCGACAGGCAACACCAGCGCTGGAGAGATTAGTTAATGCTTTGATAG
CATGA

Upstream 100 bases:

>100_bases
TGGCACAGAAATCATTGGCTCACTGATGTAAACAGGGTTTTCCTTGATTGCAATGTGCTAAGTTTGCAGCCTTACCCATA
CCGTATGGAGCGCAATAATT

Downstream 100 bases:

>100_bases
AAGAAAATATTGCAGATAGCAAAAAAGCGCCTTTAGGGCGCTTTTTTACATTGGTGGGTCGTGCAGGATTCGAACCTGCG
ACCAATTGATTAAAAGTCAA

Product: DNA-binding transcriptional activator XapR

Products: NA

Alternate protein names: Xanthosine operon regulatory protein [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MESTHRIDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKDLEQQLGTLLFVRHSRSVALTHAGKILLEESRRLLAN
ASQALARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLKECPNVHVSFREKSPGMQMALLERRELDAGIWRMAIEPTT
GFTSLRLHESSFLVAIPEDHHLACESAIPLMALRNEYFVTLPSVHSDWAFLQRVCQQAGFSPMIVREAVEPQTVLAMISM
GLGVTLMADSYAQMSWPGVVFRPLEERIPADLYIVYEQRQATPALERLVNALIA

Sequences:

>Translated_294_residues
MESTHRIDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKDLEQQLGTLLFVRHSRSVALTHAGKILLEESRRLLAN
ASQALARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLKECPNVHVSFREKSPGMQMALLERRELDAGIWRMAIEPTT
GFTSLRLHESSFLVAIPEDHHLACESAIPLMALRNEYFVTLPSVHSDWAFLQRVCQQAGFSPMIVREAVEPQTVLAMISM
GLGVTLMADSYAQMSWPGVVFRPLEERIPADLYIVYEQRQATPALERLVNALIA
>Mature_294_residues
MESTHRIDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKDLEQQLGTLLFVRHSRSVALTHAGKILLEESRRLLAN
ASQALARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLKECPNVHVSFREKSPGMQMALLERRELDAGIWRMAIEPTT
GFTSLRLHESSFLVAIPEDHHLACESAIPLMALRNEYFVTLPSVHSDWAFLQRVCQQAGFSPMIVREAVEPQTVLAMISM
GLGVTLMADSYAQMSWPGVVFRPLEERIPADLYIVYEQRQATPALERLVNALIA

Specific function: Positive regulator required for the expression of xapA and xapB. Binds to the inducer xanthosine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=292, Percent_Identity=79.4520547945205, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1787879, Length=296, Percent_Identity=36.8243243243243, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=32.0557491289199, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=30.5676855895196, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=28.2157676348548, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=24.9134948096886, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=296, Percent_Identity=26.3513513513513, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787601, Length=303, Percent_Identity=24.7524752475248, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788748, Length=239, Percent_Identity=24.2677824267782, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=221, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790262, Length=218, Percent_Identity=27.5229357798165, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=153, Percent_Identity=33.9869281045752, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=25.8196721311475, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787530, Length=242, Percent_Identity=23.1404958677686, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI48994962, Length=87, Percent_Identity=40.2298850574713, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33133; Mature: 33133

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTHRIDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKDLEQQLGTLLFVRHSRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHEEEEECCCC
VALTHAGKILLEESRRLLANASQALARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
KECPNVHVSFREKSPGMQMALLERRELDAGIWRMAIEPTTGFTSLRLHESSFLVAIPEDH
HHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCEEEEEECCCC
HLACESAIPLMALRNEYFVTLPSVHSDWAFLQRVCQQAGFSPMIVREAVEPQTVLAMISM
CEEHHHCCCHHEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHH
GLGVTLMADSYAQMSWPGVVFRPLEERIPADLYIVYEQRQATPALERLVNALIA
CCCEEEEECCHHHCCCCCCEEECHHHHCCCEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MESTHRIDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKDLEQQLGTLLFVRHSRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHEEEEECCCC
VALTHAGKILLEESRRLLANASQALARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
KECPNVHVSFREKSPGMQMALLERRELDAGIWRMAIEPTTGFTSLRLHESSFLVAIPEDH
HHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCEEEEEECCCC
HLACESAIPLMALRNEYFVTLPSVHSDWAFLQRVCQQAGFSPMIVREAVEPQTVLAMISM
CEEHHHCCCHHEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHH
GLGVTLMADSYAQMSWPGVVFRPLEERIPADLYIVYEQRQATPALERLVNALIA
CCCEEEEECCHHHCCCCCCEEECHHHHCCCEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2201776; 9205837; 9278503; 7559336 [H]