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The map label for this gene is celA

Identifier: 218700180

GI number: 218700180

Start: 1910370

End: 1911815

Strand: Reverse

Name: celA

Synonym: ECIAI39_1829

Alternate gene names: 218700180

Gene position: 1911815-1910370 (Counterclockwise)

Preceding gene: 218700181

Following gene: 218700176

Centisome position: 37.25

GC content: 45.09

Gene sequence:

>1446_bases
TTGTGTATGTCAGGATTTAAAAAAGGTTTTTTATGGGATGGCGCGGTAGCCGCGCATCAGTTGGAAGGTGGCTGGAATGA
AGGAGGAAAAGGCATCAGTATCGCCGATGTGATGACTGCTGGTGCTCACGGGGTGCCGCGTGAAGTGACAGAAGGCATTA
TCGACGGGCTTAATTATCCCAATCATGAAGCAATTGATTTTTATCATCGCTATAAAACAGATATTCAGTTATTTGCCGAG
ATGGGATTCAAATGCTTTCGAACTTCCATTGCCTGGACACGAATCTTTCCGCAAGGTGACGAACAGGAGCCGAATGAAGA
GGGTTTACAATTTTATGATGATCTGTTCGATGAATGCCTGAAGCAGGGAATGGAGCCTGTGGTGACGCTTTCGCATTTTG
AGATGCCTTATCATCTGGTGACAAAATATGGTGGCTGGCGACACCGTAAACTGATCGACTTTTTCATCCGCTTCGCATCA
ACGGTCTTCACGCGCTATAAAGAAAAAGTAAAGTACTGGATGACGTTTAACGAAATCAATAATCAGGTGAATTTCAGCGA
AAGCCTGTGTCTATTTACTAATTCCGGTATTTTGTATTCGCCAGAGGAAGATATCAATGAGCGCGAACAAATAATGTACC
AGGCGGTACATTACGAGTTAGTTGCCAGTGCCCTGGCGGTACAGACTGGAAAATCGATCAATCCTGAATTTAATATCGGC
TGCATGATCGCCATGTGCCCCATCTATCCTCTGACGTGTGCACCCAACGATATGATGATGGCGACGAAAGCGATGCATCG
TCGTTACTGGTTTACTGATGTTCATGCTCGTGGATATTATCCGCAACATATGCTGAATTACTTTGCCAGGAAAGGATTCA
ACCTCGATATCACACCAGAAGATAACGCGATTCTTGCCAGAGGTTGTGTCGACTTTATCGGCTTTAGCTACTACATGTCT
TTTACTACGCAATTTTCGCCAGATAACCCGCAACTGAATTATGTTGAACCACGAGATTTGGTCAGCAACCCTTATATCGA
TACATCCGAATGGGGATGGCAAATTGATCCGGCAGGGCTACGTTATTCACTCAACTGGTTCTGGGATCATTTCCAGTTGC
CGCTGTTTATTGTCGAAAATGGATTTGGTGCGGTTGACCAGAGACAAGCTGACGGCACGGTGAACGATCACTATCGCATT
GATTACTTTGCTTCCCATATTCGGGAAATGAAAAAAGCCGTTGTTGAAGATGGTGTTGACTTAATTGGCTACACACCGTG
GGGCTGCATTGACCTGGTTTCTGCCGGAACAGGGGAAATGAAAAAGCGCTACGGAATGATTTATGTCGATAAAGACAACG
AAGGGAAGGGAACGCTGGAACGGATACGTAAAGCGTCGTTTTACTGGTATCGGGATCTCATCGCCAACAATGGCGAAAAT
ATTTGA

Upstream 100 bases:

>100_bases
TAACGAATTATCTAATTTTAAGTTTAATGAGAACAGCAAAGACGAATTTATGGCCGGCATCCAGGCGGAAGTCTGGTGGT
AATCTATTTTAGTTAAGGAG

Downstream 100 bases:

>100_bases
GACTTCAGGAAGATATTACGGAGATGTCAGGCTCTGTGATCAGAGCCTGACAATAACTACATAGCGTGTTGATTATAATA
GGGCACGCCGAGCGCATCCG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MCMSGFKKGFLWDGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGIIDGLNYPNHEAIDFYHRYKTDIQLFAE
MGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFAS
TVFTRYKEKVKYWMTFNEINNQVNFSESLCLFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMS
FTTQFSPDNPQLNYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRI
DYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGEN
I

Sequences:

>Translated_481_residues
MCMSGFKKGFLWDGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGIIDGLNYPNHEAIDFYHRYKTDIQLFAE
MGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFAS
TVFTRYKEKVKYWMTFNEINNQVNFSESLCLFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMS
FTTQFSPDNPQLNYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRI
DYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGEN
I
>Mature_481_residues
MCMSGFKKGFLWDGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGIIDGLNYPNHEAIDFYHRYKTDIQLFAE
MGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFAS
TVFTRYKEKVKYWMTFNEINNQVNFSESLCLFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMS
FTTQFSPDNPQLNYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRI
DYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGEN
I

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=497, Percent_Identity=28.7726358148893, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI32481206, Length=501, Percent_Identity=26.5469061876248, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI13273313, Length=492, Percent_Identity=25.8130081300813, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI24497614, Length=490, Percent_Identity=30.2040816326531, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=27.0103092783505, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI2367174, Length=475, Percent_Identity=59.3684210526316, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789070, Length=483, Percent_Identity=48.24016563147, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI2367270, Length=482, Percent_Identity=50, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17552856, Length=484, Percent_Identity=28.3057851239669, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17539390, Length=488, Percent_Identity=27.4590163934426, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21356577, Length=490, Percent_Identity=28.1632653061224, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55601; Mature: 55601

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMSGFKKGFLWDGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGIIDGLNYP
CCCCCCCCCCEECCHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCCCC
NHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECL
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
HHCCCCEEEHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHC
NQVNFSESLCLFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
CCCCCCCCEEEEECCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPE
EEEEECCCCEEECCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCEEECCC
DNAILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVEPRDLVSNPYIDTSEWGWQIDPAGL
CCCEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCC
RYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVD
EEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCC
LIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGEN
EECCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
I
C
>Mature Secondary Structure
MCMSGFKKGFLWDGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGIIDGLNYP
CCCCCCCCCCEECCHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCCCC
NHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECL
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
HHCCCCEEEHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHC
NQVNFSESLCLFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIG
CCCCCCCCEEEEECCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
CMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPE
EEEEECCCCEEECCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCCEEECCC
DNAILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVEPRDLVSNPYIDTSEWGWQIDPAGL
CCCEEHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCC
RYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVD
EEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCC
LIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGEN
EECCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125345; 9384377 [H]