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The map label for this gene is insF1 [H]

Identifier: 218699889

GI number: 218699889

Start: 1584109

End: 1584549

Strand: Reverse

Name: insF1 [H]

Synonym: ECIAI39_1516

Alternate gene names: 218699889

Gene position: 1584549-1584109 (Counterclockwise)

Preceding gene: 218699890

Following gene: 218699880

Centisome position: 30.88

GC content: 50.57

Gene sequence:

>441_bases
ATGGATCTTTTTGCCCGCAGGGTTATCGGCTGGAGCCTGTCAGCGAATGCCGATACTGCACTGATAAGCAGTGCCTTGCG
GATGGCGTATGAGGTGCGTGGTCAGCCGCGGGACGTCATGTTCCATAGCGACCAGGGAAGTCAGTATACAGGACTGAAAT
ATCAACAGCTTCTCTGGCGTTACAGGATAAAGCAAAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCTATGGAA
CGCTTCTTCCGCAGTCTGAAAACAGAATGGGTGCCGACGGATGGTTACGTAGGCAAGGACGAGGCCCGGCAACAAATTAG
CGGTTATATCCTGAATTATTACAATAGCGTCAGGCCTCACCATTACAACGGTGGGTTGACGCCGGAAGAATCAGAGAACA
GATACCGTTTTTACTGTAAAACCGTGGCCAATATTACTTGA

Upstream 100 bases:

>100_bases
ACGTCCGGTTCGGGGAGGAGAGGCAGGGAAATAGTCCGACTACGCCCTGCCTCTTACTCTACTCTGGGCAGGAAATCGCT
GGTGCTATCTGGCGGTCGTC

Downstream 100 bases:

>100_bases
CCACTACAACAGCCTCATCCCGCGTGGAGTGAAGTGCATCAGTATGCAGCGTCACCAGTGCCACAACCGTGACCAGATCA
GCCGTATCAGTCACGGTATC

Product: transposase ORF B (fragment), IS3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPME
RFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT

Sequences:

>Translated_146_residues
MDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPME
RFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT
>Mature_146_residues
MDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPME
RFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=107, Percent_Identity=33.6448598130841, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788405, Length=134, Percent_Identity=40.2985074626866, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1787263, Length=134, Percent_Identity=40.2985074626866, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1786753, Length=134, Percent_Identity=40.2985074626866, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1786570, Length=134, Percent_Identity=40.2985074626866, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1786490, Length=134, Percent_Identity=40.2985074626866, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1789981, Length=134, Percent_Identity=38.8059701492537, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 17135; Mature: 17135

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
YRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPH
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
HYNGGLTPEESENRYRFYCKTVANIT
CCCCCCCCCCCCCCEEEEEEEHHCCC
>Mature Secondary Structure
MDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
YRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPH
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
HYNGGLTPEESENRYRFYCKTVANIT
CCCCCCCCCCCCCCEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]