Click here to switch to the map view.

The map label for this gene is cspF

Identifier: 218699872

GI number: 218699872

Start: 1571069

End: 1571281

Strand: Reverse

Name: cspF

Synonym: ECIAI39_1499

Alternate gene names: 218699872

Gene position: 1571281-1571069 (Counterclockwise)

Preceding gene: 218699880

Following gene: 218699871

Centisome position: 30.62

GC content: 48.36

Gene sequence:

>213_bases
TTGTCCCGTAAAATGACAGGAATTGTCAAAACCTTTGACGGCAAAAGCGGCAAGGGTCTTATCACCCCATCCGATGGTCG
TATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTT
GCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGA

Upstream 100 bases:

>100_bases
TATTTTAGACATCCAGCCATCTTCAACCCCATCAAAAAACTATAGCTTTCTTCAGGAACGTGTGTATAGTGCGCCAAGTT
ATCAGTATTAAGGAATTTTT

Downstream 100 bases:

>100_bases
GCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGA
CTGGTTGATTGGCTTTAAAA

Product: cold shock protein; Qin prophage

Products: NA

Alternate protein names: CSP-F

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFCRINGLRGPSAANVYLS

Sequences:

>Translated_70_residues
MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFCRINGLRGPSAANVYLS
>Mature_69_residues
SRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFCRINGLRGPSAANVYLS

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1787840, Length=70, Percent_Identity=100, Blast_Score=144, Evalue=1e-36,
Organism=Escherichia coli, GI2367112, Length=70, Percent_Identity=77.1428571428572, Blast_Score=115, Evalue=5e-28,
Organism=Escherichia coli, GI1787834, Length=67, Percent_Identity=47.7611940298507, Blast_Score=65, Evalue=7e-13,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=46.2686567164179, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=46.2686567164179, Blast_Score=62, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSPF_ECOL6 (P0A977)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755067.1
- ProteinModelPortal:   P0A977
- SMR:   P0A977
- EnsemblBacteria:   EBESCT00000043218
- GeneID:   1038936
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3185
- GeneTree:   EBGT00050000010463
- HOGENOM:   HBG717773
- ProtClustDB:   PRK15463
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- SMART:   SM00357

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7563; Mature: 7432

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFCRINGLR
CCCCCCEEEEECCCCCCCEEECCCCCEEEEEEEEEEECCCCCHHHCCCCEEEEEEECCCC
GPSAANVYLS
CCCCEEEEEC
>Mature Secondary Structure 
SRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFCRINGLR
CCCCCEEEEECCCCCCCEEECCCCCEEEEEEEEEEECCCCCHHHCCCCEEEEEEECCCC
GPSAANVYLS
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157