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The map label for this gene is ynfE

Identifier: 218699847

GI number: 218699847

Start: 1548009

End: 1550435

Strand: Reverse

Name: ynfE

Synonym: ECIAI39_1471

Alternate gene names: 218699847

Gene position: 1550435-1548009 (Counterclockwise)

Preceding gene: 218699848

Following gene: 218699846

Centisome position: 30.21

GC content: 50.93

Gene sequence:

>2427_bases
ATGTCCAAAAATGAACAAATGGTGGGCATCAGCCGCAGAACACTCGTTAAATCTACCGCGATAGGTTCTCTGGCGCTGGC
TGCAGGCGGTTTTTCTTTACCGTTCACCCTGCGCAGTGCAGCGGCAGCGGTACAACAGGCCAGCGAGAAAGTGGTCTGGG
GTGCCTGTTCCGTCAACTGTGGTAGCCGCTGTGCACTTCGTCTGCATGTTAAAGATAATGAAGTGACCTGGGTGGAAACT
GACAACACCGGCAACGACGAGTATGGCAATCATCAAGTACGTGCCTGTTTGCGCGGTCGCTCCATCCGTCGGCGTATTAA
TCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGCAAACGCGGCGAAGGCAAATTCGAACGGATTAGCTGGGATG
AAGCCCTGGATACTATCGCCAGTAGCCTGAAAAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCT
TCAGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCCGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGG
TTCACTCAACCAGTATGGCTCCTACAGCACCGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCA
ACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCGGCGGT
GGCATCACTTATCTTCTTGAAAAAGCGCGCGAGAAATCGAACGCAAAAATGATTGTTATCGATCCGCGATATACCGATAC
GGCTGCTGGTCGTGAAGACGAATGGCTACCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT
TGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGAT
GCACCAATAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGATGATAAAACAGCGAAAACGCCGCAGTGGGCTTC
GCAAATTACAGGTATCCCGGTAGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCC
AGGGCTGGGGGCCGCAACGCCAGGCAAACGGTGAGCTGACCGCACGCGCTATTGCTATGTTGCCTATTTTGACGGGCAAT
GTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATTGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAA
TCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACGGATGCTATCGATCATGGCCCGCAAATGACGGCAATCCGCGACG
GCGTCCGGGGCAAAGATAAACTGGATGTGCCCATTAAGTTCATCTGGAACTACGCGGGAAATACCCTGGTTAATCAGCAT
TCTGACATCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGAC
CTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACG
CCGGTAACATGGGATATCTCATTTTCCTCCAGCCAGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTG
AGTGAGGTCGCGAAACGTCTTGGACCTGACGTCTATCAAAAGTTCACAGAAGGTCGCACGCAGGAACAACGGTTACAACA
TCTGTACGCCAAAATGCTTGCCAAAGATCCGGCGTTACCGTCTTACGACGAACTGAAAAAAATGGGTATCTATAAGCGTA
AAGATCCCAATGGTCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGT
AAGATTGAAATTTATTCCAGCAAGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCACT
GCCGGTTTATGCCTCAACCTTTGAAGGCTGGGACTCCCCTGAACGTAGTACCTTTCCACTGCAACTGTTTGGTTTCCATT
ACAAATCCCGTACGCACTCGACCTACGGCAACATTGATGTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCT
ATAGACGCACAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTATAACCATCGTGGCGAAGTTCGGCTACCAGC
CAAAGTGACACCACGCATTCTCCCTGGGGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATA
AAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCCTCACCACTGGCGAAGGGAAACCCGCAGCACACT
AATCTGGTCGAGATCGAAAAAATATAA

Upstream 100 bases:

>100_bases
ATAATTCTTAACCCAAAATAGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATATAACGA
TAAAGAACAGGGAGTGAGTT

Downstream 100 bases:

>100_bases
CCCTCTACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGA
GGCGCTCATGAAGGCTGAAA

Product: oxidoreductase subunit

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGNDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APINGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARELTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQRLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI

Sequences:

>Translated_808_residues
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGNDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APINGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARELTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQRLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI
>Mature_807_residues
SKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETD
NTGNDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSS
GIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGG
ITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADA
PINGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNV
GISGGNSGARELTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQHS
DINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILS
EVAKRLGPDVYQKFTEGRTQEQRLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGK
IEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPI
DAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTN
LVEIEKI

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=808, Percent_Identity=98.3910891089109, Blast_Score=1656, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=801, Percent_Identity=70.6616729088639, Blast_Score=1222, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=805, Percent_Identity=66.9565217391304, Blast_Score=1128, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.4116865869854, Blast_Score=285, Evalue=6e-78,
Organism=Escherichia coli, GI87081994, Length=848, Percent_Identity=28.6556603773585, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1787231, Length=760, Percent_Identity=29.2105263157895, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI3868721, Length=787, Percent_Identity=24.0152477763659, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI3868720, Length=457, Percent_Identity=25.601750547046, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787741, Length=307, Percent_Identity=27.6872964169381, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI3868719, Length=457, Percent_Identity=24.72647702407, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788534, Length=882, Percent_Identity=22.7891156462585, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89541; Mature: 89409

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVVWGACSVNC
CCCCCCEECCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPINGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARELTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCEEEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQRLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEEECCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVVWGACSVNC
CCCCCEECCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPINGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARELTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCEEEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQRLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEEECCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]