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The map label for this gene is ynjE

Identifier: 218699676

GI number: 218699676

Start: 1362749

End: 1364056

Strand: Reverse

Name: ynjE

Synonym: ECIAI39_1297

Alternate gene names: 218699676

Gene position: 1364056-1362749 (Counterclockwise)

Preceding gene: 218699677

Following gene: 218699674

Centisome position: 26.58

GC content: 53.98

Gene sequence:

>1308_bases
ATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGC
GAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATTGATACTCGCCCCAGCGCGTTTTATAACGGCT
GGCCACAAACCTTAAATGGCCCTTCCGGTCATGAACCTGCCGCCTTAAACCTCTCTGCTAGCTGGCTCGACAAAATGAGC
ACCGAACAGCTCAACGCGTGGATCAAGCAACATAACCTGAAAGCCGATGCCCCGGTGGCGCTGTACGGTAATGGCAAAGA
TGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTTACGCATATCTCCATTCTGAGTGACGCGCTAAGCGAACCTT
CCCGTCTGCAAAAACTGCCGCATTTTGAGCAGCTGGTTTATCCGCAATGGCTGCATGACCTGCAACAAGGTAAAGAGGTT
ACGGCGAAACCTGCCGGTGACTGGAAAGTCATTGAAGCGGCCTGGGGCGCTCCTAAGCTTTACCTTATCAGCCATATTCC
CGGCGCTGACTACATCGACACCAACGAAGTGGAAAGCGAACCGCTGTGGAACAAAGTTTCTGATGAACAACTGAAAGCGA
TGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTACGGGCGTGACGTATACGCTGCAGCGCGTGTGGCG
CAGATTATGCTTTATGCCGGCGTGAAAGATGTGCGCCTGCTGGACGGAGGCTGGCAAACCTGGTCCGACGCGGGTCTGCC
CGTTGAGCGCGGTACGCCACCGAAAGTGAAAGCGGAACCAGATTTCGGCGTGAAGATCCCGGCACAACCGCAACTGATGC
TTGATATGGAACAAGCGCGTGGACTGCTGCATCGCCAGGATGCATCGCTGGTGAGCATTCGTTCGTGGCCAGAATTTATC
GGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGAGCACGTTGGGGACACGCTGGTAGCGACTC
GACGCATATGGAAGATTTCCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACGGCTATGTGGAAAGCATGGA
ATATCAGACCAGATCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGAGCA
ATGGGCTGGAATAACGTCTCCGTTTACGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAATCCGGTAGCAACCGG
TGAACGCGGCCCGGACAGCAGCAAATAA

Upstream 100 bases:

>100_bases
GCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAA
CTACAATGTCGGGATTTTCG

Downstream 100 bases:

>100_bases
CATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAA
CGCACCAAATACCCACGCAA

Product: putative exported thiosulfate sulfur transferase / thiosulfate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Sequences:

>Translated_435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK
>Mature_435_residues
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMS
TEQLNAWIKQHNLKADAPVALYGNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFI
GTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRASETFMYARA
MGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=302, Percent_Identity=26.4900662251656, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI61835204, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194473681, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194473668, Length=300, Percent_Identity=24.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87081967, Length=435, Percent_Identity=98.3908045977011, Blast_Score=883, Evalue=0.0,
Organism=Escherichia coli, GI87082121, Length=249, Percent_Identity=22.8915662650602, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 48159; Mature: 48159

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNGKDVDAVKTRLQKAGF
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDITAMWKAWNIRPDQQVSFYCGTGWRASETFMYARAMGWNNVSVYDGGWYEWSSDP
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEHHHCCCCCEEEECCCEEECCCCC
KNPVATGERGPDSSK
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCCCCCCC
PSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNGKDVDAVKTRLQKAGF
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
THISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAWGAPKL
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEE
YLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQAR
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEEHHHHC
GLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTM
CHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
RSADDITAMWKAWNIRPDQQVSFYCGTGWRASETFMYARAMGWNNVSVYDGGWYEWSSDP
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEHHHCCCCCEEEECCCEEECCCCC
KNPVATGERGPDSSK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]