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The map label for this gene is irp

Identifier: 218699464

GI number: 218699464

Start: 1160094

End: 1161398

Strand: Direct

Name: irp

Synonym: ECIAI39_1080

Alternate gene names: 218699464

Gene position: 1160094-1161398 (Clockwise)

Preceding gene: 218699463

Following gene: 218699471

Centisome position: 22.6

GC content: 55.17

Gene sequence:

>1305_bases
ATGAAAATCAGTGAATTTCTACATCTGGCGTTACCAGAGGAACAATGGCTACCGACGATTTCTGGCGTTTTACGCCAGTT
CGCAGAAGAAGAGTGTTATGTCTATGAGCGTCAACCCTGTTGGTATTTAGGCAAAGGGTGCCAGGCACGGCTGCACATTA
ATGCCGACGGAACGCAGGCGACATTTATTGATGATGCCGGGGAGCAAAAATGGGCGGTGGATTCCATTGCCGACTGCGCG
CGTCGTTTTATGGCGCATCCTCAGGTGAAAGGACGTCGGGTATATGGACAGGTTGGGTTCAACTTTGCGGCGCATGCGCG
GGGGATTGCCTTTAACGTCGGGGAGTGGCCGCTGCTGACGTTAACCGTTCCCCGTGAAGAACTTATTTTTGAAAAGGGAA
ATGTCACCGTTTATGCGGACTCCGCCGACGGGTGCCGACGTTTGTGCGAGTGGGTAAAAGAGGCCGGTACAACGACGCAG
AACGCACCACTGGCGGTGGATACCGCCCTCAATGGTGAGACATATAAACAACAGGTTGCGCGCGCCGTTGCGGAGATCCG
CCGTGGCGAGTATGTCAAAGTGATTGTCTCGCGCGCCATTCCCCTGCCATCGCGGATTGATATGCCCGCCACGCTGTTAT
ACGGGCGGCAGGCAAACACGCCTGTGCGCTCGTTTATGTTCCGTCAGGAAGGACGCGAAGCGCTGGGCTTTAGCCCGGAA
CTGGTGATGTCAGTGACGGGCAATAAAGTGGTCACTGAACCGCTTGCGGGCACCCGCGATCGCATGGGAAACCCGGAGCA
TAATAAGGCGAAAGAGGCAGAACTGCTGCACGACAGTAAAGAGGTGCTTGAGCATATCCTTTCTGTCAAAGAAGCTATTG
CTGAACTGGAGGCCGTTTGCCAGCCGGGCAGCGTGGTGGTTGAAGATTTAATGTCGGTTCGCCAGCGCGGCAGCGTTCAG
CATCTGGGGTCTGGCGTGAGCGGTCAGCTTGCGGAAAACAAGGATGCCTGGGATGCGTTTACCGTGCTGTTTCCGTCGAT
TACCGCCTCAGGTATCCCTAAAAATGCTGCTCTGAACGCGATTATGCAAATTGAGAAGACGCCGCGAGAGCTTTATTCCG
GCGCAATCCTGCTGCTGGACGATACGCGCTTTGATGCGGCGCTAGTTCTGCGTTCCGTATTTCAGGATAGCCAGCGCTGC
TGGATACAGGCGGGGGCGGGAATCATCGCGCAATCTACGCCGGAACGCGAACTGACAGAAACCCGGGAGAAATTAGCGAG
CATTGCGCCCTATTTAATGGTGTAG

Upstream 100 bases:

>100_bases
GTTGCTGGCGGCGTGGGTTGCTTATATCCGGCTGCATTCGGCAAGAGAACTGATGACAAGCGCAATTGATTGATCCGCGA
ATAACACAGAGAGGTAAGGG

Downstream 100 bases:

>100_bases
GAGTAACTGTTTTCTAACCTCACAACCAGATTGTCCAAAGACAATTGAGTCGCTCTTTGATTCCTCTGTGACTCTATTGT
CTTCTTTTGGGATAAAATAG

Product: salicylate synthase Irp9

Products: NA

Alternate protein names: Mycobactin synthase protein I; Salicylate synthase mbtI [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLGKGCQARLHINADGTQATFIDDAGEQKWAVDSIADCA
RRFMAHPQVKGRRVYGQVGFNFAAHARGIAFNVGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
NAPLAVDTALNGETYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQANTPVRSFMFRQEGREALGFSPE
LVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELLHDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQ
HLGSGVSGQLAENKDAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRSVFQDSQRC
WIQAGAGIIAQSTPERELTETREKLASIAPYLMV

Sequences:

>Translated_434_residues
MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLGKGCQARLHINADGTQATFIDDAGEQKWAVDSIADCA
RRFMAHPQVKGRRVYGQVGFNFAAHARGIAFNVGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
NAPLAVDTALNGETYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQANTPVRSFMFRQEGREALGFSPE
LVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELLHDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQ
HLGSGVSGQLAENKDAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRSVFQDSQRC
WIQAGAGIIAQSTPERELTETREKLASIAPYLMV
>Mature_434_residues
MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLGKGCQARLHINADGTQATFIDDAGEQKWAVDSIADCA
RRFMAHPQVKGRRVYGQVGFNFAAHARGIAFNVGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
NAPLAVDTALNGETYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQANTPVRSFMFRQEGREALGFSPE
LVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELLHDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQ
HLGSGVSGQLAENKDAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRSVFQDSQRC
WIQAGAGIIAQSTPERELTETREKLASIAPYLMV

Specific function: Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicyl

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family. Salicylate synthase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=251, Percent_Identity=33.4661354581673, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788114, Length=355, Percent_Identity=23.3802816901408, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082077, Length=265, Percent_Identity=28.6792452830189, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786809, Length=267, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=256, Percent_Identity=30.078125, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR015890
- InterPro:   IPR019996 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48046; Mature: 48046

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLGKGCQARLHINADGTQA
CCHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCEEEECCCCEEECCCCEEEEEEECCCCEE
TFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNFAAHARGIAFNVGEWPLLT
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCEEEECCCCEEEE
LTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQNAPLAVDTALNGETYKQQVA
EECCHHHHEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
RAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQANTPVRSFMFRQEGREALGFSPE
HHHHHHHCCCCEEEHHHHCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHCCHHHCCCCCC
LVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELLHDSKEVLEHILSVKEAIAELEAVC
EEEEECCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENKDAWDAFTVLFPSITASGIPKNAALNA
CCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
IMQIEKTPRELYSGAILLLDDTRFDAALVLRSVFQDSQRCWIQAGAGIIAQSTPERELTE
HHHHHCCHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHEEHHCCCCEEECCCCCHHHHH
TREKLASIAPYLMV
HHHHHHHHCCHHCC
>Mature Secondary Structure
MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLGKGCQARLHINADGTQA
CCHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCEEEECCCCEEECCCCEEEEEEECCCCEE
TFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNFAAHARGIAFNVGEWPLLT
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCEEEECCCCEEEE
LTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQNAPLAVDTALNGETYKQQVA
EECCHHHHEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
RAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQANTPVRSFMFRQEGREALGFSPE
HHHHHHHCCCCEEEHHHHCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHCCHHHCCCCCC
LVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELLHDSKEVLEHILSVKEAIAELEAVC
EEEEECCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENKDAWDAFTVLFPSITASGIPKNAALNA
CCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
IMQIEKTPRELYSGAILLLDDTRFDAALVLRSVFQDSQRCWIQAGAGIIAQSTPERELTE
HHHHHCCHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHEEHHCCCCEEECCCCCHHHHH
TREKLASIAPYLMV
HHHHHHHHCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA