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The map label for this gene is ybiY

Identifier: 218699197

GI number: 218699197

Start: 847260

End: 848186

Strand: Reverse

Name: ybiY

Synonym: ECIAI39_0801

Alternate gene names: 218699197

Gene position: 848186-847260 (Counterclockwise)

Preceding gene: 218699199

Following gene: 218699196

Centisome position: 16.53

GC content: 54.05

Gene sequence:

>927_bases
ATGTTTGAACGAAACAGAGAGGCAACTATGATTTTCAATATTCAGCGCTACTCGACCCATGATGGCCCCGGTATCCGCAC
GGTCGTATTTCTTAAAGGCTGTTCGCTGGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGCGCCCGCACGCAGGATCTGT
TGTATGACGCACGACTGTGTCTGGAAGGCTGCGAGCTGTGCGCTAAGGCCGCGCCGGACGTGATTGAGCGCGCGTTGAAT
GGTTTGCTTATTCATCGGGAAAAGTTAACCCCGGAGCATCTGACGGCGCTAACCGACTGCTGCCCGACACAGGCGTTAAC
CGTGTGTGGTGAAGTGAAAAGCGTTGAGGAGATCATGGCGACCGTTTTGCGCGATAAACCGTTTTACGATCGCAGCGGCG
GCGGCTTAACACTTTCTGGCGGTGAACCGTTTATGCAGTCGGAAATGGCGACGGCGCTACTGCAAGCCAGCCACGAGGCC
GGCATTCATACTGCGGTAGAAACCTGTCTGCATGTGCCGTGGAAATATATCTCCCCTTCTCTGCCCTATATCGATCTGTT
TCTTGCCGATTTAAAACACGTTGCCGACGCGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGGGTGCTGGATAACC
TGAAAAAACTCGCCGCAGCGGGCAAAAAAATCATTATCCGCGTGCCACTGATTCAGGGCTTTAATGCCGACGAAACCGCT
GTAAAAGCCATTACCGATTTTGCCGCCGACGAGCTGCACGTTAGCGAAATTCATTTCCTGCCCTACCACACGCTGGGCAT
CAACAAATATCACTTACTTAATCTGCCCTATGACGCCCCGGAACAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCC
AGCAGTATGCCTGCCAGAAAGGGTTAACCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
CATCCTCAAAATGTACTTTTTGTCTGAATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACGTACATCAATCTAAC
TTTCATTCGAAAGTATTTTA

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACACTCAGCGACCGCATTAAAGTGCACAAAAATGCGCTGGTGCATATTGTGAAA
CCGCCGGTTTGTACCGAGCG

Product: putative AdoMet-dependent glycyl radical activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQSEMATALLQASHEA
GIHTAVETCLHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETA
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQSEMATALLQASHEA
GIHTAVETCLHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETA
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLDAPELLDFAQQYACQKGLTATLRG
>Mature_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPDVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQSEMATALLQASHEA
GIHTAVETCLHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETA
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=97.3244147157191, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1790389, Length=308, Percent_Identity=35.7142857142857, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=32.1637426900585, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790839, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 34120; Mature: 34120

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKAAPDVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCCHHHHHHHHHHHEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQSEMATALLQASHEAGIHTAVETCLHVPWKYISPS
HHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETA
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLDAPELLDFAQQYACQK
HHHHHHHHHCCEEHHHEEECCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC
>Mature Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKAAPDVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHCCHHHHHHHHHHHEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQSEMATALLQASHEAGIHTAVETCLHVPWKYISPS
HHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETA
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
VKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLDAPELLDFAQQYACQK
HHHHHHHHHCCEEHHHEEECCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]