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The map label for this gene is ybiW

Identifier: 218699196

GI number: 218699196

Start: 844822

End: 847254

Strand: Reverse

Name: ybiW

Synonym: ECIAI39_0800

Alternate gene names: 218699196

Gene position: 847254-844822 (Counterclockwise)

Preceding gene: 218699197

Following gene: 218699195

Centisome position: 16.51

GC content: 54.83

Gene sequence:

>2433_bases
ATGACCACACTGAAACTGGACACACTCAGCGACCGCATTAAAGTGCACAAAAATGCGCTGGTGCATATTGTGAAACCGCC
GGTTTGTACCGAGCGCGCGCAGCACTATACCGGGATGTATCAACAACATCTCGATAAGCCGATCCCGGTACGTCGCGCGC
TGGCACTGGCGCATCACCTGGCGAATCGCACCATCTGGATCAAGCACGATGAGTTGATCATCGGCAACCAGGCAAGCGAG
GTTCGCGCCGCGCCGATCTTCCCGGAATATACCGTCTCGTGGATCGAAAAAGAGATTGATGATCTGGCAGATCGTCCGGG
TGCTGGTTTTGCGGTGAGCGAGGAGAACAAACGTGTTCTGCATGAAGTGTGCCCGTGGTGGCGCGGTCAGACCGTACAGG
ATCGCTGCTACGGCATGTTTACCGATGAGCAAAAAGGTCTGCTGGCGACCGGCATCATTAAAGCGGAGGGCAATATGACC
TCCGGCGATGCGCACCTGGCGGTGAATTTCCCGCTACTGCTGGAAAAAGGGCTTGACGGTCTGCGTGAGAAAGTGGCGGA
ACGTCGCTCGCGCATCAATCTGACGGTACTGGAAGATCTGCACGGCGAGCAATTCCTGAAAGCGATTGATATCGTGCTGG
TGGCAGTCAGTGAACACATTGAACGTTTCGCTGCCCTGGCGCGTGAAATGGCCGCGACCGAAACCCGCGAAAGCCGTCGC
GATGAACTGCTGACGATAGCAGAAAACTGCGATCTTATCGCCCACCAGCCGCCGCAGACTTTCTGGCAGGCGCTGCAACT
GTGTTACTTCATCCAGTTGATTTTGCAGATCGAATCTAACGGTCACTCGGTATCGTTTGGTCGTATGGACCAGTATCTCT
ACCCATACTATCGCCGCGACGTTGAACTGAACCAGACACTGGATCGCGAACATGCCATCGAGATGCTGCATAGCTGCTGG
TTGAAATTGCTGGAAGTGAACAAAATCCGCTCCGGCTCACACTCAAAAGCCTCTGCGGGAAGTCCGCTGTATCAGAACGT
CACCATTGGCGGGCAAAATCTGGTTGATGGTCAACCGATGGACGCGGTAAATCCACTCTCTTATGCGATCCTCGAATCTT
GCGGTCGCCTGCGTTCCACTCAGCCTAACCTCAGCGTGCGCTACCACGCGGGAATGAGCAACGATTTCCTCGATGCCTGT
GTTCAGGTGATCCGCTGTGGTTTCGGGATGCCGGCGTTCAACAACGACGAAATCGTCATCCCGGAATTTATTAAACTCGG
TATTGAACCGCAGGACGCTTACGACTACGCAGCGATTGGTTGTATCGAAACCGCCGTCGGTGGCAAATGGGGCTATCGCT
GTACCGGCATGAGCTTTATCAACTTCGCCCGCGTGATGCTGGCGGCGCTGGAAGGCGGTCGTGATGCCACCAGCGGCAAA
GTGTTCCTGCCACAAGAAAAAGCGTTGTCAGCGGGTAACTTCAACAACTTCGATGAAGTAATGGACGCATGGGATACGCA
AATCCGTTACTACACGCGTAAATCAATCGAAATCGAATATGTCGTCGACACCATGCTGGAAGAGAACGTACACGATATTC
TCTGCTCGGCGCTGGTGGATGACTGCATTGAGCGAGCGAAAAGTATCAAACAAGGCGGCGCGAAGTATGACTGGGTGTCT
GGCTTGCAGGTCGGTATCGCCAACCTCGGCAACAGCCTGGCGGCAGTGAAGAAACTGGTATTTGAACAGGGCGCGATTGG
TCAGCAACAGCTGGCTGCCGCACTGGCGGATGACTTCGACGGCCTGACTCACGAGCAGCTGCGTCAGCGGCTGATTAACG
GTGCGCCTAAGTACGGCAACGACGATGATACTGTCGATACGCTGCTGGCTCGCGCTTATCAGACCTATATCGACGAACTA
AAACAGTACCATAACCCGCGCTATGGTCGTGGTCCGGTTGGCGGCAATTATTACGCGGGTACGTCGTCTATCTCCGCTAA
CGTACCGTTTGGCGCGCAAACTATGGCAACGCCGGACGGGCGTAAAGCGCATACCCCGCTGGCAGAAGGCGCTAGTCCGG
CCTCCGGTACTGACCATCTCGGCCCTACTGCGGTCATTGGCTCAGTGGGTAAACTGCCTACGGCAGCGATTCTCGGCGGC
GTGTTGCTCAACCAGAAACTGAATCCGGCAACGCTGGAGAACGAATCTGACAAGCAGAAACTGATGATCCTGCTACGTAC
CTTCTTTGAGGTGCATAAAGGCTGGCATATTCAGTACAACATCGTTTCCCGCGAAACGCTGCTGGAAGCGAAAAAACATC
CGGATCAGTACCGCGATCTGGTGGTGCGCGTAGCGGGTTATTCCGCCTTCTTCACCGCGCTCTCTCCAGACGCACAGGAC
GATATCATCGCCCGTACTGAACATATGCTGTAA

Upstream 100 bases:

>100_bases
ACGCCCCGGAACAACCGCTTGATGCGCCAGAACTGCTCGACTTTGCCCAGCAGTATGCCTGCCAGAAAGGGTTAACCGCG
ACCTTACGAGGATAACAACC

Downstream 100 bases:

>100_bases
TCTTCTCAGCCCGGCGTCGTCGTCGCCGGGCTAAATCATTCACATCATCAATTTCATCCTTACTTTCATTCGAAATATAA
TTTGTGCTCTGCGTCACATT

Product: putative glycyl radical cofactor protein

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTTLKLDTLSDRIKVHKNALVHIVKPPVCTERAQHYTGMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
DELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML

Sequences:

>Translated_810_residues
MTTLKLDTLSDRIKVHKNALVHIVKPPVCTERAQHYTGMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASE
VRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
DELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCW
LKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDAC
VQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVS
GLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDEL
KQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQD
DIIARTEHML
>Mature_809_residues
TTLKLDTLSDRIKVHKNALVHIVKPPVCTERAQHYTGMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEV
RAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTS
GDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRRD
ELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWL
KLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACV
QVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGKV
FLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSG
LQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELK
QYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGV
LLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDD
IIARTEHML

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=99.1358024691358, Blast_Score=1674, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=805, Percent_Identity=38.0124223602485, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI48994926, Length=564, Percent_Identity=28.3687943262411, Blast_Score=180, Evalue=4e-46,
Organism=Escherichia coli, GI1787131, Length=563, Percent_Identity=27.3534635879218, Blast_Score=179, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90127; Mature: 89995

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLKLDTLSDRIKVHKNALVHIVKPPVCTERAQHYTGMYQQHLDKPIPVRRALALAHHL
CCEEEEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
HEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHH
LREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHEEHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TTLKLDTLSDRIKVHKNALVHIVKPPVCTERAQHYTGMYQQHLDKPIPVRRALALAHHL
CEEEEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFAVSEENKRVL
HCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHH
HEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDG
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECHHHHHHHHHH
LREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETRESRR
HHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRD
HHHEEHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
VELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLVDGQPM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
DAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVI
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEC
PEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCC
VFLPQEKALSAGNFNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVD
EECCHHHHCCCCCCCCHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHH
DCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFD
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAG
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
TSSISANVPFGAQTMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
VLLNQKLNPATLENESDKQKLMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHH
VVRVAGYSAFFTALSPDAQDDIIARTEHML
HHHHHCHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]