Click here to switch to the map view.

The map label for this gene is speF

Identifier: 218699048

GI number: 218699048

Start: 687402

End: 689600

Strand: Reverse

Name: speF

Synonym: ECIAI39_0651

Alternate gene names: 218699048

Gene position: 689600-687402 (Counterclockwise)

Preceding gene: 218699050

Following gene: 218699047

Centisome position: 13.44

GC content: 48.98

Gene sequence:

>2199_bases
ATGTCAAAATTAAAAATTGCGGTTAGTGATTCTTGCCCGGACTGTTTTACCACGCAGCGAGAATGTATCTATATTAATGA
AAACAGTAATATCGATGTGGCGGCAATAGTTTTATCGCTCAACGATGTTACACGCGGAAAACTCGATGAAATCGATGCCA
CGGGTTATGGCATCCCGGTATTTATTGCTACTGAAAATCAAGAACGTGTACCCGCAGAGTATTTGCCCCGTATTTCGGGT
GTCTTTGAGAATTGCGAATCGCGACGAGAATTTTATGGTCGTCAGTTAGAAACCGCAGCCAGCCATTATGAAACTCAACT
GCGCCCGCCCTTCTTCCGCGCACTGGTCGATTATGTCAATCAGGGTAACAGCGCGTTTGATTGCCCCGGTCATCAGGGCG
GCGAATTTTTCCGTCGCCATCCGGCGGGGAATCAGTTTGTGGAATACTTTGGTGAGGCGCTGTTCCGTGCTGACTTGTGC
AACGCGGATGTGGCAATGGGCGATCTGCTGATTCACGAAGGCGCGCCATGCATTGCACAGCAACATGCGGCAAAAGTGTT
TAATGCCGATAAAACATACTTCGTTCTAAATGGCACTTCATCTTCTAACAAAGTGGTTTTAAACGCCCTGTTAACACCGG
GGGATCTGGTGCTGTTTGACCGTAATAACCATAAATCTAATCACCACGGAGCGTTGCTACAGGCTGGCGCAACACCAGTT
TATCTGGAAACCGCACGCAACCCGTATGGCTTTATCGGTGGTATTGATGCCCACTGCTTCGAAGAAAATTACCTGCGCGA
ACTGATTACAGAAGTCGCACCGCAGCGGGCAAAAGAGGCGCGTCCTTTCCGCCTCGCCGTTATTCAGCTAGGCACTTACG
ACGGTACGATTTATAACGCCCGTCAGGTGGTGGATAAAATTGGTCATCTGTGTGACTACATCCTCTTTGACTCCGCATGG
GTCGGCTATGAGCAGTTTATCCCGATGATGGCGGACTGTTCACCACTGTTGCTGGATCTTAATGAGAACGATCCGGGTAT
TCTGGTTACGCAATCTGTGCATAAACAGCAGGCTGGTTTTTCCCAGACTTCACAAATCCATAAAAAAGACAGCCATATCA
AAGGGCAACAGCGTTATGTACCGCACAAACGCATGAACAACGCCTTTATGATGCATGCCTCCACCAGCCCGTTCTATCCG
CTGTTTGCCGCACTGGATATCAACGCCAAAATGCATGAAGGTGTCAGCGGTCGTAATATGTGGATGGATTGTGTGGTAAA
TGGCATTAATGCCCGCAAACTGCTCCTCGATAACTGTCAGCATATTCGTCCATTCGTACCTGAACTGGTGGATGGTAAAC
CCTGGCAGTCGTATGAAACAGCGCAAATTGCGGTTGATCTGCGCTTCTTCCAGTTTGTACCAGGTGAGCACTGGCATTCT
TTTGAAGGCTATGCAGAGAATCAATACTTTGTCGATCCGTGCAAACTGTTGCTGACAACCCCAGGTATTGATGCACGTAA
CGGCGAATATGAAGCGTTCGGTGTACCCGCGACGATTCTTGCTAACTTCCTGCGCGAAAATGGCGTAGTGCCGGAAAAAT
GCGATCTTAACTCCATCCTCTTTCTGCTGACTCCGGCAGAAGATATGGCCAAACTTCAGCAACTTGCTGCTCTGCTGGTA
CGCTTCGAAAAACTGCTTGAGTCCGACGCGCCATTATCAGAAGTGCTGCCTTCCATCTACAAACAGCATGAAGAGCGCTA
CACCGGTTATACCCTGCGTCAGTTGTGTCAGGAAATGCATGATTTGTATGCCCGTCACAACGTGAAACAACTGCAAAAAG
AGATGTTTCGTAAGGAACACTTCCCACGTGTCAGCATGAATCCACAAGAAGCCAACTACGCCTATTTACGCGGTGAAGTG
GAGCTGGTTCGTCTGCCGGATGCAGAAGGCCGTATCGCTGCAGAAGGTGCGCTTCCTTATCCTCCGGGTGTGCTGTGTGT
TGTTCCGGGTGAAATCTGGGGTGGTGCTGTTCTGCGTTACTTCAGCGCTCTGGAAGAAGGGATCAACCTGCTGCCAGGTT
TTGCACCGGAGCTTCAGGGTGTCTATATCGAAGAACATGATGGTCGTAAGCAAGTTTGGTGCTATGTCATCAAGCCTCGT
GATGCGCAAAGCATCCTGTTGAAAGGGGAAAAATTATGA

Upstream 100 bases:

>100_bases
TTAATTTATCAGCAGGGTAACTTTGCTTTTTTTCCGGCACGATCGATTTCTCATTCGAGAAATTGAGGACCTGCTATTAC
CAAAAATAAAGAGTTGGAAA

Downstream 100 bases:

>100_bases
GTCAGGCTAAATCGAACAAAATGGGCGTCGTTCAGTTAACCATACTGACGATGGTCAACATGATGGGCTCCGGTATCATC
ATGCTGCCGACAAAGCTTGC

Product: ornithine decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVTRGKLDEIDATGYGIPVFIATENQERVPAEYLPRISG
VFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
YLETARNPYGFIGGIDAHCFEENYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAW
VGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYP
LFAALDINAKMHEGVSGRNMWMDCVVNGINARKLLLDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
FEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLAALLV
RFEKLLESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEV
ELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
DAQSILLKGEKL

Sequences:

>Translated_732_residues
MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVTRGKLDEIDATGYGIPVFIATENQERVPAEYLPRISG
VFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
YLETARNPYGFIGGIDAHCFEENYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAW
VGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYP
LFAALDINAKMHEGVSGRNMWMDCVVNGINARKLLLDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
FEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLAALLV
RFEKLLESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEV
ELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
DAQSILLKGEKL
>Mature_731_residues
SKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVTRGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGV
FENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLCN
ADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPVY
LETARNPYGFIGGIDAHCFEENYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWV
GYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPL
FAALDINAKMHEGVSGRNMWMDCVVNGINARKLLLDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHSF
EGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLAALLVR
FEKLLESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVE
LVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRD
AQSILLKGEKL

Specific function: Spermidine biosynthesis from ornithine. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786909, Length=732, Percent_Identity=98.4972677595628, Blast_Score=1507, Evalue=0.0,
Organism=Escherichia coli, GI87082193, Length=719, Percent_Identity=66.759388038943, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI221142684, Length=678, Percent_Identity=31.2684365781711, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1790573, Length=636, Percent_Identity=33.3333333333333, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1786384, Length=692, Percent_Identity=31.2138728323699, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR005308
- InterPro:   IPR011193
- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C; PF03709 OKR_DC_1_N [H]

EC number: =4.1.1.17 [H]

Molecular weight: Translated: 82516; Mature: 82385

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00703 OKR_DC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVTRGKLDEIDATGYGIPV
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
FIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVN
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLCNADVAMGDLLIHEGAPCIAQ
CCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCHHHH
QHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEE
YLETARNPYGFIGGIDAHCFEENYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNA
EEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEEEEECCCCCCHHH
RQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCC
SQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNM
HHHHHHHHHHCCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCCCCH
WMDCVVNGINARKLLLDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCCCCC
FEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSIL
CCCCCCCCEEECCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEE
FLLTPAEDMAKLQQLAALLVRFEKLLESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMH
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPY
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEECCCCCC
PPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCC
DAQSILLKGEKL
CCHHHHCCCCCC
>Mature Secondary Structure 
SKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVTRGKLDEIDATGYGIPV
CCEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
FIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVN
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLCNADVAMGDLLIHEGAPCIAQ
CCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCHHHH
QHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEE
YLETARNPYGFIGGIDAHCFEENYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNA
EEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEEEEECCCCCCHHH
RQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCC
SQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNM
HHHHHHHHHHCCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCCCCH
WMDCVVNGINARKLLLDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCCCCC
FEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSIL
CCCCCCCCEEECCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEE
FLLTPAEDMAKLQQLAALLVRFEKLLESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMH
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPY
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEECCCCCC
PPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCC
DAQSILLKGEKL
CCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939141; 8905232; 9278503 [H]