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The map label for this gene is citT

Identifier: 218698985

GI number: 218698985

Start: 623179

End: 624642

Strand: Reverse

Name: citT

Synonym: ECIAI39_0588

Alternate gene names: 218698985

Gene position: 624642-623179 (Counterclockwise)

Preceding gene: 218698986

Following gene: 218698984

Centisome position: 12.17

GC content: 50.82

Gene sequence:

>1464_bases
ATGTCTTTAGCAAAAGATAATATATGGAAATTATTGGCGCCCCTGGTGGTGATGGGTGTCATGTTTCTTATCCCTGTACC
TGACGGTATGCCGCCGCAGGCGTGGCATTACTTTGCAGTGTTTGTGGCAATGATTGTCGGCATGATCCTCGAGCCAATTC
CGGCAACGGCGATCAGTTTTATTGCGGTTACTATTTGCGTTATTGGCAGTAATTACCTGCTCTTTGATGCCAAAGAATTA
GCTGACCCAGCGTTTAATGCGCAAAAACAGGCGCTGAAATGGGGTCTGGCTGGTTTTTCCAGCACCACGGTATGGCTGGT
ATTTGGCGCATTTATTTTTGCATTAGGGTATGAAGTTTCCGGGTTAGGTCGTCGCATTGCCCTTTTCCTGGTGAAATTCA
TGGGCAAACGCACGCTGACGTTGGGTTATGCGATTGTCATTATCGACATTCTGCTGGCACCGTTTACACCGTCCAACACC
GCGCGTACCGGGGGTACGGTTTTTCCGGTCATTAAAAACCTGCCGCCGCTGTTTAAATCATTCCCGAACGATCCGTCCGC
GCGTCGTATTGGCGGCTATTTGATGTGGATGATGGTCATTAGTACCAGTCTGAGTTCGTCCATGTTTGTCACCGGTGCGG
CACCAAACGTGCTGGGTCTGGAGTTCGTCAGCAAAATTGCCGGTATCCAGATTAGCTGGTTGCAGTGGTTCCTCTGCTTC
CTGCCGGTTGGGGTTATCTTGCTTATCATTGCGCCGTGGCTTTCCTACGTGCTGTACAAACCGGAAATCACACACAGTGA
AGAAGTGGCAACCTGGGCGGGTGATGAACTAAAAACCATGGGTGCACTGACGCGCAGAGAGTGGACGCTGATTGGCCTTG
TATTGCTCAGCTTAGGTTTGTGGGTATTTGGCAGTGAAGTCATTAATGCTACTGCGGTAGGTCTGCTGGCAGTTTCGCTG
ATGCTGGCGCTGCACGTTGTGCCGTGGAAAGACATTACTCGCTATAACAGCGCATGGAACACGCTGGTCAACCTGGCAAC
GCTGGTTGTTATGGCGAATGGCCTGACCCGTTCTGGCTTTATCGACTGGTTCGCCAACACGATGAGTACACACCTGGAAG
GCTTCTCACCAAACGCAACGGTGATTGTACTGGTTCTGGTGTTCTACTTTGCACACTACCTGTTTGCCAGCCTGTCTGCG
CACACCGCAACCATGCTGCCGGTTATTCTGGCCGTCGGTAAAGGTATTCCGGGCGTACCAATGGAACAACTGTGTATTCT
GCTGGTGCTGTCTATCGGTATCATGGGCTGTCTGACGCCGTATGCAACCGGTCCTGGGGTGATTATTTACGGCTGTGGCT
ATGTGAAATCAAAAGATTACTGGCGACTTGGTGCAATCTTCGGGGTGATTTACATCTCTATGCTGCTGTTGGTTGGCTGG
CCGATTCTCGCCATGTGGAACTAA

Upstream 100 bases:

>100_bases
GGCGGCAGTGCCGACCTGCTGATTCTTACCTGGTTTTTAGCACAGATTTAATAATTTAAGCACTTGATAAAATTGGAATA
TTAATTGTCGGAGAACCCGT

Downstream 100 bases:

>100_bases
TACGTTCGCCAAACGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAATAGATA
GTATTCAGTATATGTATGAG

Product: citrate:succinate antiporter

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN

Sequences:

>Translated_487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN
>Mature_486_residues
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELA
DPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTA
RTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCFL
PVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLM
LALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAH
TATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWP
ILAMWN

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=487, Percent_Identity=99.7946611909651, Blast_Score=965, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=471, Percent_Identity=44.7983014861996, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786986, Length=493, Percent_Identity=33.4685598377282, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 53150; Mature: 53019

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCC
>Mature Secondary Structure 
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]