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The map label for this gene is rna

Identifier: 218698984

GI number: 218698984

Start: 622259

End: 623065

Strand: Reverse

Name: rna

Synonym: ECIAI39_0587

Alternate gene names: 218698984

Gene position: 623065-622259 (Counterclockwise)

Preceding gene: 218698985

Following gene: 218698983

Centisome position: 12.14

GC content: 51.43

Gene sequence:

>807_bases
ATGAAAGCATTCTGGCGTAACGCCGCGTTGCTCGCGGTTTCTCTGCTTCCCTTCTCTTCTGCCAACGCCGTGGCGTTGCA
GGCAAAACAGTATGGCGATTTTGATCGCTATGTCCTGGCCCTCTCCTGGCAAACCGGATTTTGCCAGAGTCAACACGATC
GAAATCGTAACGAACGAGATGAATGTCGCCTGCAAACCGAAACGACCAACAAAGCTGATTTTCTGACCGTACATGGTCTG
TGGCCAGGATTGCCTAAATCGGTTGCTGCCCGTGGTGTTGATGAACGCCGCTGGATGCGCTTCGGTTGCGCTACTCGCCC
AATCCCGAATCTACCAGAAGCGCGCGCCAGCCGAATGTGTTCATCTCCGGAAACCGGGTTATCGCTGGAAACGGCAGCTA
AACTAAGTGAAGTCATGCCAGGAGCTGGCGGACGTTCCTGCCTGGAACGCTACGAATATGCCAAACACGGTGCCTGCTTT
GGTTTTGATCCAGACGTATACTTCGGCACGATGGTACGCCTGAATCAAGAAATTAAAGAGAGCGAAGCCGGAAAATTCCT
TGCGGATAACTACGGTAAAACAGTGAGCCGCCGTGACTTTGATGCCGCCTTTGCCAAAAGCTGGGGAAAAGAGAACGTGA
AAGCAGTTAAGCTAACGTGCCAGGGTAACCCTGCGTATTTGACTGAAATTCAGATCTCGATCAAAGCTGACGCCATCAAC
GCTCCGCTTTCTGCCAACTCATTCTTGCCACAACCACACCCAGGCAACTGTGGCAAAACCTTTGTGATTGATAAAGCAGG
TTATTAA

Upstream 100 bases:

>100_bases
CGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAATAGATAGTATTCAGTATAT
GTATGAGTTCCACACCCATT

Downstream 100 bases:

>100_bases
TCCTCTGATTTACAAAACACGCTAATTCGTCTTCCATAAACCATGACGATTAAGATTTATACATAATGCGCTACATTGAG
GAATTCCCTGAATGTGACGC

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDVYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY

Sequences:

>Translated_268_residues
MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDVYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY
>Mature_268_residues
MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDVYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=268, Percent_Identity=99.2537313432836, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 29632; Mature: 29632

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERD
CCCHHHHHHEEEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCH
ECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMC
HHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDVYFGTMVRLNQEIKE
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
HHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCC
APLSANSFLPQPHPGNCGKTFVIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERD
CCCHHHHHHEEEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCH
ECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMC
HHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDVYFGTMVRLNQEIKE
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
HHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCC
APLSANSFLPQPHPGNCGKTFVIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]