| Definition | Escherichia coli IAI39 chromosome, complete genome. |
|---|---|
| Accession | NC_011750 |
| Length | 5,132,068 |
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The map label for this gene is dkgB
Identifier: 218698846
GI number: 218698846
Start: 476305
End: 477108
Strand: Reverse
Name: dkgB
Synonym: ECIAI39_0442
Alternate gene names: 218698846
Gene position: 477108-476305 (Counterclockwise)
Preceding gene: 218698847
Following gene: 218698844
Centisome position: 9.3
GC content: 46.52
Gene sequence:
>804_bases ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGAAAACGGCGCTTGA ACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCG TGCCACGTAATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGACCCCAAGTCTGAAA GAGAGCCTGCAAAAATTGCGTATCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGT TGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCC CATTGATGGAAAAGGCGATTGCTGCTGTTGGCGCTGAAAACATCGCTACTAACCAGATTGAACTCTCACCTTATCTGCAA AACCGTAAAGTGGTCGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCT GAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGG AAGGTTACTCCGTAATTCCTTCTTCTACTAAACGTAAAAACCTGGAAAGTAATCTTAAGGCACAAAATTTACAGCTTGAT GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAAGGTCTGGCTCCTGAATGGGA TTAA
Upstream 100 bases:
>100_bases TAAATCGCAAAAATCCTCTGCGTTTTACGCCCTTTTTCCGCAACATTCTAAAGCCCATAATCACTTCAGTTAACGAAAAT AGCATTAAAAGAGGCATATT
Downstream 100 bases:
>100_bases GCCTCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGTGTACTTAC CGCACGGTCGCTGTAATATA
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLTPSLK ESLQKLRIDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD
Sequences:
>Translated_267_residues MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLTPSLK ESLQKLRIDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD >Mature_266_residues AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLTPSLKE SLQKLRIDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQN RKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDA EDKKAIAALDCNDRLVSPEGLAPEWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=32, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=30.4794520547945, Blast_Score=142, Evalue=4e-34, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=30.4794520547945, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=30.4794520547945, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=30.1038062283737, Blast_Score=141, Evalue=8e-34, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=30.1038062283737, Blast_Score=141, Evalue=8e-34, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=30.1369863013699, Blast_Score=140, Evalue=2e-33, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=31.0580204778157, Blast_Score=138, Evalue=5e-33, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.553264604811, Blast_Score=137, Evalue=8e-33, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=30.6397306397306, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.5263157894737, Blast_Score=133, Evalue=1e-31, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.1003344481605, Blast_Score=133, Evalue=2e-31, Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29, Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.4832713754647, Blast_Score=126, Evalue=2e-29, Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=29.2592592592593, Blast_Score=121, Evalue=7e-28, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.5755395683453, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=37.719298245614, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=37.719298245614, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=6e-14, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=98.501872659176, Blast_Score=535, Evalue=1e-154, Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=38.671875, Blast_Score=184, Evalue=7e-48, Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=28.2527881040892, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI1787674, Length=257, Percent_Identity=27.6264591439689, Blast_Score=76, Evalue=3e-15, Organism=Escherichia coli, GI48994888, Length=271, Percent_Identity=28.4132841328413, Blast_Score=73, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=35.7933579335793, Blast_Score=156, Evalue=1e-38, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.8620689655172, Blast_Score=146, Evalue=9e-36, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.7638888888889, Blast_Score=144, Evalue=3e-35, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=31.203007518797, Blast_Score=137, Evalue=7e-33, Organism=Caenorhabditis elegans, GI17552492, Length=267, Percent_Identity=31.0861423220974, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=30.7971014492754, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=30.0724637681159, Blast_Score=126, Evalue=1e-29, Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=31.6793893129771, Blast_Score=122, Evalue=3e-28, Organism=Caenorhabditis elegans, GI17538386, Length=276, Percent_Identity=29.7101449275362, Blast_Score=120, Evalue=6e-28, Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=27.0547945205479, Blast_Score=119, Evalue=2e-27, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.8707482993197, Blast_Score=117, Evalue=8e-27, Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=28.996282527881, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI212645785, Length=140, Percent_Identity=30, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6324694, Length=276, Percent_Identity=36.231884057971, Blast_Score=161, Evalue=9e-41, Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.1702127659575, Blast_Score=157, Evalue=2e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=29.4117647058824, Blast_Score=130, Evalue=1e-31, Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=2e-28, Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=30.9090909090909, Blast_Score=112, Evalue=7e-26, Organism=Saccharomyces cerevisiae, GI6320079, Length=271, Percent_Identity=29.1512915129151, Blast_Score=96, Evalue=6e-21, Organism=Drosophila melanogaster, GI24662789, Length=290, Percent_Identity=31.0344827586207, Blast_Score=145, Evalue=4e-35, Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=32.6530612244898, Blast_Score=144, Evalue=6e-35, Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=31.7073170731707, Blast_Score=138, Evalue=4e-33, Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=30.8917197452229, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.4685314685315, Blast_Score=132, Evalue=2e-31, Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=31.4878892733564, Blast_Score=130, Evalue=9e-31, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.8245614035088, Blast_Score=130, Evalue=1e-30, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.2560553633218, Blast_Score=125, Evalue=3e-29, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.5640138408304, Blast_Score=121, Evalue=6e-28, Organism=Drosophila melanogaster, GI45553081, Length=283, Percent_Identity=28.2685512367491, Blast_Score=117, Evalue=8e-27, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=26.7441860465116, Blast_Score=97, Evalue=8e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29429; Mature: 29298
Theoretical pI: Translated: 5.43; Mature: 5.43
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELY CCCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCCCCEE ITTKIWIENLSKDKLTPSLKESLQKLRIDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC >Mature Secondary Structure AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRNELY CCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCCCCEE ITTKIWIENLSKDKLTPSLKESLQKLRIDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]