Click here to switch to the map view.

The map label for this gene is est [H]

Identifier: 218697601

GI number: 218697601

Start: 4458930

End: 4459913

Strand: Reverse

Name: est [H]

Synonym: EC55989_4363

Alternate gene names: 218697601

Gene position: 4459913-4458930 (Counterclockwise)

Preceding gene: 218697606

Following gene: 218697590

Centisome position: 86.52

GC content: 51.52

Gene sequence:

>984_bases
GTGAATTTCCCTGTATCCATCCAGCAAAAACGGGTGTATAAGACTGTACATTCATACAGCAAAATAAGTTCTACTATGGC
ACTGGAAAAAGGAATTGCAGAGTTAGTCGAAAAGTTTATCGCTGCGGGTCGCCCCTCATCGCGTCAACAAAGTATTGCGC
AGCGCAGGGAGGGCTATATCGCCAGCGCTGTCCTTGCGGGTGAGACAGAAACGCGGGTAGATATTCAGACTATCGAACTT
GAAGGGATGACCTTACGAATAGTGTCGCCGCTGAATGCCCCGACATTACTCCCGACGATAATTTATTACTACGGTGGCTG
TTTTGTTAGCGGCGGGTTCGCAACTCACGACAACCAGCTGCGCCAGTTAGCCTATTACGGGCAATGTCGGGTTATTGCCG
TCCAGTATCGGCTGGCACCGGAACATACTTTCCCCGCCGCCCATGATGATGCGCAACGAGGTGCAGAAATAGTCTGGCAA
CACGCCGAACGACTCGGCGTTGATAAACAACGTATAACTCTTGCCGGTGACAGTGCGGGCGGACATCTGGCGCTGGTAAC
TGCTTTGCGATTAAAGCGTGCTGGCGAGTGGCAACCGGCACAACTCATCTTAATTTATCCTATGCTTGATGCGACTGCGC
ATTTCGAAAGCTACATACGCAATGGTCACGATTACATCATCACACGCGACACTTTACTGAGCGGCTTTGAGATGTATCTG
CCAGGCATTGAACGCAGGCATCCTGAAGCCAGCCCTCTTTGGCGCGATGATTTCAATGGCTTGCCACCTGTACATATCAT
TACCGCTGAATACGACCCACTTTGCGATGAAGGTGAAGCGTTGTATCACCGCATGACAGGGCAAGGAGTGCAATGCACCT
GCCAACGCTACCTTGGCGTTATCCACGGATTTTTTCAGTTAGGTGGTATCAGCGAGGCCGCGCGATCGGCACTACGGGAT
GTTGCCTGGAGAGCGGGAAGGTAA

Upstream 100 bases:

>100_bases
CCCTTGAAACCACTAGCTTACTGATAGTGCTCCAGCAAAACCACGTCTCTGCCGGGTTTGTCTTTAGATTTGCGAGGCGC
TTTCCAGATTTTTCACGCCC

Downstream 100 bases:

>100_bases
CTTCGGACCTGGGCCCTGAAAAGGTTTTCAGATTGAAAACCTTTTCAGTCAAGTAAAACGCCCCTACTCTTCTTCGTTCC
CGCCCTCTTCATACGGGCCA

Product: putative lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNFPVSIQQKRVYKTVHSYSKISSTMALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR

Sequences:

>Translated_327_residues
MNFPVSIQQKRVYKTVHSYSKISSTMALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR
>Mature_327_residues
MNFPVSIQQKRVYKTVHSYSKISSTMALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.4436090225564, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI21328446, Length=118, Percent_Identity=34.7457627118644, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=279, Percent_Identity=31.1827956989247, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=26.7924528301887, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=200, Percent_Identity=28, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=113, Percent_Identity=33.6283185840708, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=148, Percent_Identity=30.4054054054054, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=133, Percent_Identity=29.3233082706767, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=133, Percent_Identity=29.3233082706767, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 36518; Mature: 36518

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPVSIQQKRVYKTVHSYSKISSTMALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
ASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQL
EEEEEECCCCCEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHH
RQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAG
HHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
PGIERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
IHGFFQLGGISEAARSALRDVAWRAGR
HHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFPVSIQQKRVYKTVHSYSKISSTMALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
ASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQL
EEEEEECCCCCEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHH
RQLAYYGQCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAG
HHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
PGIERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
IHGFFQLGGISEAARSALRDVAWRAGR
HHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]