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The map label for this gene is pldB

Identifier: 218697546

GI number: 218697546

Start: 4393346

End: 4394368

Strand: Direct

Name: pldB

Synonym: EC55989_4302

Alternate gene names: 218697546

Gene position: 4393346-4394368 (Clockwise)

Preceding gene: 218697544

Following gene: 218697547

Centisome position: 85.23

GC content: 53.57

Gene sequence:

>1023_bases
ATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTG
GCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATG
ACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTG
GGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGT
TAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCT
ATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTATGTGACGCCATTGCG
CTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCA
TCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACA
GCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGG
GTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTACAAGC
TGAAGAGGAACGCGTGGTGGATAACCGCATGCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAG
GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC
GCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAA

Upstream 100 bases:

>100_bases
CGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGG
TTTAAGAGGCAGATTACCCG

Downstream 100 bases:

>100_bases
ATTTCTTATGTACCAGGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCA
AAGAAACTCTGAAGCTGCTC

Product: lysophospholipase L2

Products: NA

Alternate protein names: Lecithinase B

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRMHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV

Sequences:

>Translated_340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRMHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV
>Mature_340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRMHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=340, Percent_Identity=100, Blast_Score=702, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLDB_ECOL6 (P59588)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756606.1
- ProteinModelPortal:   P59588
- EnsemblBacteria:   EBESCT00000043181
- GeneID:   1035613
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4747
- GeneTree:   EBGT00050000009455
- HOGENOM:   HBG748003
- OMA:   DPVRYAR
- ProtClustDB:   PRK10749
- BRENDA:   3.1.1.5
- InterPro:   IPR000073

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.5

Molecular weight: Translated: 38979; Mature: 38979

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVIC
CCCCCCCCHHHHCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAF
CCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
WQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIALTAPMFGIVIRMPSFMARQI
HHHHCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
HHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHH
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRMHDRFCELRTAAGHPVEGGRPLVI
HHHHHHCCCHHEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
KGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSGNRSTEV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVIC
CCCCCCCCHHHHCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAF
CCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
WQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIALTAPMFGIVIRMPSFMARQI
HHHHCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
HHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHH
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRMHDRFCELRTAAGHPVEGGRPLVI
HHHHHHCCCHHEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
KGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSGNRSTEV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157