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The map label for this gene is ygbJ

Identifier: 218696330

GI number: 218696330

Start: 3075053

End: 3075961

Strand: Direct

Name: ygbJ

Synonym: EC55989_3003

Alternate gene names: 218696330

Gene position: 3075053-3075961 (Clockwise)

Preceding gene: 218696328

Following gene: 218696331

Centisome position: 59.65

GC content: 53.03

Gene sequence:

>909_bases
ATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATGTGTCCG
CGCAGGTCTTTCTACCTGGGGCGCAGACCTGAACAGCAATGCCTGCGCTACGTTGAAAGAGGCAGGTGCTTGCGGGGTTT
CTGATAACGCCGCGACGTTTGCCGAAAAACTGGACGCTCTGCTGGTGCTGGTGGTCAATGCGGCCCAGGTTAAACAGGTG
CTGTTTGGTGAAACAGGCGTTGCACAACATCTGAAACCCGGTACGGCAGTAATGGTTTCTTCCACTATCGCTAGTGCTGA
TGCGCAAGAAATTGCTACCGCTCTGGCTGGATTCGATCTGGAAATGCTGGATGCGCCAGTTTCTGGTGGTGCAGTAAAAG
CCGCTAACGGTGAAATGACTGTCATGGCCTCCGGTAGCGATATTGCCTTTGAACGACTGGCACCCGTGCTGGAAGCCGTT
GCCGGAAAAGTTTATCGCATAGGTGCAGAACCGGGACTAGGTTCGACCGTAAAAATTATTCACCAGTTGTTAGCGGGCGT
ACATATTGCTGCCGGAGCCGAAGCGATGGCACTTGCAGCCCGTGCGGGGATCCCGCTGGATGTGATGTATGACGTCGTGA
CCAATGCCGCCGGAAATTCCTGGATGTTCGAAAACCGGATGCGTCATGTGGTGGATGGCGATTACACCCCGCATTCAGCC
GTCGATATTTTTGTTAAGGATCTTGGTCTGGTTGCCGATACAGCCAAAGCCCTGCACTTCCCGCTGCCATTGGCCTCAAC
AGCATTGAATATGTTCACCAGCGCCAGTAACGCAGGTTACGGGAAAGAAGACGATAGCGCAGTTATCAAGATTTTCTCTG
GCATCACTCTACCGGGAGCGAAATCATGA

Upstream 100 bases:

>100_bases
CAATGTTAATTTATGTGGATCAGCTCACCATATCGTTATCTTCCAGCGCTTATATTCACAATATAAAACAAAATATCACT
TAAATTAACAAGGAGAGCAG

Downstream 100 bases:

>100_bases
TCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACG
GTACAAATTAACGGTGTTCC

Product: putative dehydrogenase, with NAD(P)-binding Rossmann-fold domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACATLKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQV
LFGETGVAQHLKPGTAVMVSSTIASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSA
VDIFVKDLGLVADTAKALHFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGAKS

Sequences:

>Translated_302_residues
MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACATLKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQV
LFGETGVAQHLKPGTAVMVSSTIASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSA
VDIFVKDLGLVADTAKALHFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGAKS
>Mature_302_residues
MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACATLKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQV
LFGETGVAQHLKPGTAVMVSSTIASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSA
VDIFVKDLGLVADTAKALHFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGAKS

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=29.1525423728814, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI40556376, Length=290, Percent_Identity=22.7586206896552, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1789092, Length=302, Percent_Identity=99.6688741721854, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=34.3971631205674, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=29.7945205479452, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=28.3216783216783, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=28.7822878228782, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574115, Length=289, Percent_Identity=28.0276816608997, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 30691; Mature: 30691

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACATLKEAGACGVSDNAATF
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHH
AEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSSTIASADAQEIATALAGFDL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHCCCH
EMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAVAGKVYRIGAEPGLGSTVKII
HHHCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
HQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSA
HHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHH
VDIFVKDLGLVADTAKALHFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
KS
CC
>Mature Secondary Structure
MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACATLKEAGACGVSDNAATF
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHH
AEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSSTIASADAQEIATALAGFDL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHCCCH
EMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAVAGKVYRIGAEPGLGSTVKII
HHHCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
HQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSA
HHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHH
VDIFVKDLGLVADTAKALHFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]