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The map label for this gene is pphB

Identifier: 218696328

GI number: 218696328

Start: 3073383

End: 3074039

Strand: Direct

Name: pphB

Synonym: EC55989_3001

Alternate gene names: 218696328

Gene position: 3073383-3074039 (Clockwise)

Preceding gene: 218696327

Following gene: 218696330

Centisome position: 59.62

GC content: 42.62

Gene sequence:

>657_bases
ATGCCATCTACACGCTATCAAAAAATTAATGCCCATCACTATCGCCATATATGGGTCGTTGGTGATATTCATGGTGAATA
TCAATTATTACAATCCCGCTTACATCAACTCTCTTTTTGCCCTGAAACCGACTTACTTATTTCTGTCGGCGATAATATTG
ATCGTGGGCCGGAGAGTCTTGACGTCCTGCGCCTGCTAAACCAGCCCTGGTTTATCTCCGTTAAAGGCAACCACGAAGCA
ATGGCGCTGGATGCGTTCGCGACCGGCGATGGCAATGTGTGGCTTGCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGA
TTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACA
TAAAATATGTCATCGCACATGCAGATTATCCGGGTGATGAATATCTCTTTGGCAAAGAAATAGCGGAGAGCGAATTACTC
TGGCCTGTTTATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACA
TATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAAAAGTGGGCGGCTGTCAT
TTTATAAAATAAGGTAG

Upstream 100 bases:

>100_bases
ATTCCCGATTGTCTTTTGCTATCGGGAATATTAACGACAACTGACGAATAAAATAAAAACACCCTGTATAATAGGAAAGC
TTATTTTACAGGGTAAAACC

Downstream 100 bases:

>100_bases
TCTCATGCTTCTTCTGTGAAGCATGAGTAACCAGATGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAG
ATCCTGCTTCGCCAGCGCGC

Product: serine/threonine-specific protein phosphatase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEA
MALDAFATGDGNVWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGDEYLFGKEIAESELL
WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR

Sequences:

>Translated_218_residues
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEA
MALDAFATGDGNVWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGDEYLFGKEIAESELL
WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
>Mature_217_residues
PSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAM
ALDAFATGDGNVWLASGGDWFFDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGDEYLFGKEIAESELLW
PVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR

Specific function: Has been shown, in vitro, to act on Ser, Thr and Tyr- phosphorylated substrates [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1789090, Length=218, Percent_Identity=94.4954128440367, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI87081988, Length=217, Percent_Identity=45.6221198156682, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25098; Mature: 24966

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHH
DVLRLLNQPWFISVKGNHEAMALDAFATGDGNVWLASGGDWFFDLNDSEQQEAIDLLLKF
HHHHHHCCCEEEEEECCCCEEEEEEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEITNDNIKYVIAHADYPGDEYLFGKEIAESELLWPVYRVQKSLNGELQQINGA
HCCCEEEEEECCCEEEEEEECCCCCCCEEECHHHCCCHHHHHHHHHHHHCCCCEEECCCC
DYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
CEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
>Mature Secondary Structure 
PSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHH
DVLRLLNQPWFISVKGNHEAMALDAFATGDGNVWLASGGDWFFDLNDSEQQEAIDLLLKF
HHHHHHCCCEEEEEECCCCEEEEEEEECCCCCEEEECCCCEEEECCCCHHHHHHHHHHHH
HHLPHIIEITNDNIKYVIAHADYPGDEYLFGKEIAESELLWPVYRVQKSLNGELQQINGA
HCCCEEEEEECCCEEEEEEECCCCCCCEEECHHHCCCHHHHHHHHHHHHCCCCEEECCCC
DYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
CEEEEHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9278503 [H]