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The map label for this gene is hypA

Identifier: 218696320

GI number: 218696320

Start: 3064270

End: 3064620

Strand: Direct

Name: hypA

Synonym: EC55989_2993

Alternate gene names: 218696320

Gene position: 3064270-3064620 (Clockwise)

Preceding gene: 218696307

Following gene: 218696321

Centisome position: 59.44

GC content: 52.71

Gene sequence:

>351_bases
ATGCACGAAATAACCCTCTGCCAACGGGCACTGGAATTGATCGAACAGCAGGCCGCAAAACACGGCGCAAAACGCGTAAC
TGGGGTCTGGCTCAAAATTGGCGCATTTTCTTGTGTCGAAACCAGCTCTCTTGCCTTTTGTTTTGATCTGGTTTGCCGCG
GCAGCGTGGCGGAAGGTTGTAAACTGCACCTCGAAGAACAAGAGGCCGAATGCTGGTGTGAAACATGCCAACAGTATGTG
ACGCTACTGACCCAGCGCGTCCGCCGCTGTCCACAGTGTCATGGTGACATGCTACAGATTGTGGCAGACGACGGTTTACA
GATTCGGCGGATAGAAATAGACCAGGAGTGA

Upstream 100 bases:

>100_bases
TCGACGTGTCATTTCGACATCATCGACATTATTCACCGCAGGGATAATCAACACTGGCACAATTATTGCTTGTAGCTGGC
AATAGTTAATGGGAGGCGAT

Downstream 100 bases:

>100_bases
GCGATGTGTACAACATGCGGTTGCGGTGAAGGCAACCTGTATATCGAGGGTGATGAACATAACCCTCATTCCGCGTTTCG
TAGCGCGCCATTTGCCCCGG

Product: hydrogenase nickel incorporation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYV
TLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE

Sequences:

>Translated_116_residues
MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYV
TLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE
>Mature_116_residues
MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYV
TLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE

Specific function: Not required for the formation of hydrogenases 1 and 2. Plays probably a regulatory role

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

Organism=Escherichia coli, GI1789081, Length=116, Percent_Identity=100, Blast_Score=234, Evalue=1e-63,
Organism=Escherichia coli, GI1789365, Length=114, Percent_Identity=48.2456140350877, Blast_Score=117, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPA_ECO57 (P0A701)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F85921
- PIR:   F91076
- RefSeq:   NP_289276.1
- RefSeq:   NP_311609.1
- ProteinModelPortal:   P0A701
- SMR:   P0A701
- EnsemblBacteria:   EBESCT00000025816
- EnsemblBacteria:   EBESCT00000058486
- GeneID:   914696
- GeneID:   958185
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4035
- KEGG:   ecs:ECs3582
- GeneTree:   EBGT00050000009287
- HOGENOM:   HBG688671
- OMA:   CFELVCR
- ProtClustDB:   PRK03681
- BioCyc:   ECOL83334:ECS3582-MONOMER
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 13168; Mature: 13168

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
10.3 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHH
KLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGSVAEGC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHH
KLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796