Click here to switch to the map view.

The map label for this gene is ascB

Identifier: 218696307

GI number: 218696307

Start: 3052092

End: 3053516

Strand: Direct

Name: ascB

Synonym: EC55989_2978

Alternate gene names: 218696307

Gene position: 3052092-3053516 (Clockwise)

Preceding gene: 218696306

Following gene: 218696320

Centisome position: 59.21

GC content: 52.07

Gene sequence:

>1425_bases
ATGTCAGTATTTCCAGAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGG
CAAAGGACTGACAACGGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTC
AGTTGCGCGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGATTCAAAGTGTTCCGTACCTCAATTGCCTGGAGCCGCCTCTTCCCGCAGGGCGATGAACTGACGCCCAA
CCAGCAGGGCATTGCTTTTTACCGTTCTGTCTTTGAAGAGTGTAAAAAGTATGGTATCGAACCGCTGGTCACGTTGTGCC
ACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGCTTTTCAGCCGCTAC
GCCCGGACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTAACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGC
TGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGT
AACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGATCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGCGGCGCGTATCCGGCTTACTCTGCCCGCGTATTTCGCGAAAAAGGGGTAACCATCAACAAAGCACCGG
GCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGG
AATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACG
GCCTCGGCGCAAAAGATGAACTTGCTGCCAATGGCGAGATTAACGATGACTATCGCATCAGCTATTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGATGGCATTCCGCTGATGGGCTACACCACCTGGGGCTGTATTGATTTAGTTTCCGC
CTCTACGGGTGAAATGAGCAAACGCTACGGTTTTGTCTACGTAGACCGTGACGACGCAGGCAACGGCACGCTGACGCGCA
CGCGTAAGAAATCGTTCTGGTGGTATAAAAAAGTAATTGCCAGTAATGGGGAAGATTTAGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGGCTGCCGAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACCGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAGTTCGTGCGACATGTAGATACTGTTATGGCCTGCA
AGTTTTTTCCTGGTCCGGTT

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMA
EMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMN
ANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=30.2605210420842, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI32481206, Length=496, Percent_Identity=29.2338709677419, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=27.7207392197125, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=26.5848670756646, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=24.0325865580448, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=98.5232067510548, Blast_Score=983, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.6413502109705, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.4644351464435, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17552856, Length=485, Percent_Identity=30.1030927835052, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=492, Percent_Identity=29.8780487804878, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21356577, Length=503, Percent_Identity=25.0497017892644, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53765; Mature: 53634

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTINKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTINKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]