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The map label for this gene is yfiE

Identifier: 218696205

GI number: 218696205

Start: 2937676

End: 2938557

Strand: Reverse

Name: yfiE

Synonym: EC55989_2866

Alternate gene names: 218696205

Gene position: 2938557-2937676 (Counterclockwise)

Preceding gene: 218696207

Following gene: 218696203

Centisome position: 57.01

GC content: 49.21

Gene sequence:

>882_bases
ATGGATCTGCGCCGTTTTATTACACTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
TACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAGCAGGAGTTCTCGGTACAATTGTTTGAGAAAATTGGTCGAC
GAATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTGCCGCACATCTATGAACTTACCCGAGTGATGGATACGCTTCGTGAG
GCTGCGAAAAAAGAGTCGGATCCGGACGGGGAACTACGCGTTGTTTCAGGCGAAACGTTGCTCTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCTCCAAAAGTGCGTTTATCGTTGCAGTCGCTGAACTGTTACGTGATCCGTGATG
CGCTACTGAATGATGAGGCCGATGTCGGGGTTTTTTATCGCGTAGGGAATGATGATGTTCTGAACCGACGAGAGTTGGGT
GAACAACCGCTGGTGCTGGTGGCTTCACCGCAAATTGCAGATATCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAG
CTTTATTATCAACGAACCACAATGTGTCTTCCGGCAGATATTTGAGAGCACGCTGCGCCAGCGGCGGATCACGGTGGAAA
ACACCATTGAGCTGTTAAGCATTGAAAGTATCAAACGTTGTGTCGCGGCGAATATCGGTGTCAGTTACTTGCCCCGCTTT
GCGGTGGAAAAAGAGCTTGAGAGCGGAGAATTAATTGAATTGCCTTTTGGCGAACAGTCGCAGACCATTACGGCCATGTG
CGCACACCATGCTGGAAAAGCGGTTAGCCCGGCGATGCACACTTTTATTCAGTGTGTTGAAGAGAGTTTTGTGGCTGGAT
AA

Upstream 100 bases:

>100_bases
GTTATCTCCTCTGTTTTGGTAGACAGTACCCGACACGGAACATTTAAAAAAATGAATGTTTTTACATTCTAAATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
AAATAATGTCGGGATTAAACCCGGCATTTAACTTGTCGACAAAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCT
TATCCGGCCTACAAGATCGT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLPRF
AVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG

Sequences:

>Translated_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLPRF
AVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
>Mature_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLPRF
AVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=97.9522184300341, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1788481, Length=302, Percent_Identity=25.8278145695364, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787806, Length=291, Percent_Identity=23.7113402061856, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=26.8370607028754, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=23.6749116607774, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=31.3868613138686, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.7042253521127, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082024, Length=271, Percent_Identity=25.4612546125461, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=134, Percent_Identity=34.3283582089552, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=24, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33338; Mature: 33338

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELGEQPLVLVASPQIADIDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCHHCHHHCCCCCEEEEECCCEEECCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELGEQPLVLVASPQIADIDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCHHCHHHCCCCCEEEEECCCEEECCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]