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The map label for this gene is yfiC

Identifier: 218696203

GI number: 218696203

Start: 2935264

End: 2936001

Strand: Reverse

Name: yfiC

Synonym: EC55989_2864

Alternate gene names: 218696203

Gene position: 2936001-2935264 (Counterclockwise)

Preceding gene: 218696205

Following gene: 218696201

Centisome position: 56.96

GC content: 53.52

Gene sequence:

>738_bases
ATGTCACAGTCTACATCCGTGCTTCGTCGTAATGGATTTACTTTTAAACAGTTTTTTGTTGCTCACGATCGCTGTGCGAT
GAAAGTGGGAACGGATGGCATTTTGCTGGGCGCATGGGCACCGGTGGCTGGGGTGAAACGTTGCCTTGATATCGGCGCGG
GTAGCGGGTTGCTGGCATTAATGCTGGCGCAGCGAACCGATGACAGCGTGATAATTGATGCCGTTGAGCTGGAAAGTGAA
GCTGCCGCGCAGGCGCAGGAAAATATCAACCAGTCCCCGTGGGCAGAGCGGATTAACGTCCATACGGCGGATATTCAGCA
GTGGATCACACAGCAGACAGTACGCTTCGATTTAATCATCAGTAACCCACCTTACTATCAGCAGGGAGTAGAGTGCGCGA
CCCCTCAACGGGAACAGGCTCGCTATACCACTACGCTTGATCACCCATCGCTGCTGACCTGTGCGGCGGAGTGCATTACC
GAAGAGGGATTTTTCTGCGTAGTGCTGCCAGAGCAGATTGGTAATGGTTTTACGGAGCTGGCATTAAGCATGGGCTGGCA
TTTACGTTTGCGGACGGATGTGGCGGAAAACGAAGCGCGACTGCCGCATCGGGTGCTGCTGGCATTCTCCCCGCAGGCGG
GAGAATGCTTTAGCGATCGCTTAGTCATTCGTGGGCCAGACCAGAACTATTCCGAAGCGTATACGGCGCTGACCCAGGCT
TTTTATCTGTTTATGTAA

Upstream 100 bases:

>100_bases
ACGTTACGGCTGCAATCTTCATCTGTTTGTATGGATATCGCTTCTCGGGTATTGTTCTCACCCGGTGCTATCGCCGTTTT
TCCTGCAAGGTTTTATTTTC

Downstream 100 bases:

>100_bases
TGATTGCCGGGGGAAAGGATCGACGGACCGGAATGGGTGAGCAGTTCCGGATAATCCAGCGTGAAATGCAGGCCACGACT
CTCCTTGCGCATCATCGCAC

Product: putative S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESE
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM

Sequences:

>Translated_245_residues
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESE
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM
>Mature_244_residues
SQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESEA
AAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITE
EGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAF
YLFM

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val)

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082131, Length=245, Percent_Identity=99.1836734693878, Blast_Score=509, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMN6_ECO24 (A7ZQ20)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463899.1
- ProteinModelPortal:   A7ZQ20
- SMR:   A7ZQ20
- STRING:   A7ZQ20
- EnsemblBacteria:   EBESCT00000019992
- GeneID:   5589589
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2861
- NMPDR:   fig|331111.3.peg.590
- eggNOG:   COG4123
- GeneTree:   EBGT00050000010470
- HOGENOM:   HBG307609
- OMA:   FKQFHIN
- ProtClustDB:   CLSK880437
- BioCyc:   ECOL331111:ECE24377A_2861-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01872
- InterPro:   IPR002052
- InterPro:   IPR007848
- InterPro:   IPR022882

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.55

Molecular weight: Translated: 27236; Mature: 27105

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
CCCCHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
MLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
HHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEE
SNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
CCCCHHHCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHH
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
HHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
FYLFM
HHHCC
>Mature Secondary Structure 
SQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
CCCHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
MLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
HHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEE
SNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
CCCCHHHCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHH
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
HHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
FYLFM
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA