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The map label for this gene is wcaK

Identifier: 218695668

GI number: 218695668

Start: 2347264

End: 2348544

Strand: Reverse

Name: wcaK

Synonym: EC55989_2301

Alternate gene names: 218695668

Gene position: 2348544-2347264 (Counterclockwise)

Preceding gene: 218695669

Following gene: 218695667

Centisome position: 45.56

GC content: 54.33

Gene sequence:

>1281_bases
ATGAAATTACTTATTCTGGGCAACCACACTTGCGGCAATCGTGGCGACAGCGCCATCCTGCGCGGCTTACTTGATGCCAT
CAACATCCTCAATCCACAAGCCGAAGTGGACGTGATGAGCCGCTATCCGGTCAGTTCTTCCTGGCTGCTCAACCGCCCGG
TAATGGGTGATCCGCTGTTCCTTCAAATGAAACAACACAACAGCGCGGCGGGCGTTGTCGGGCGCGTTAAAAAAGTCCTC
CGTCGCCGCTACCAGCACCAGGTACTGCTCTCGCGCGTCACCGACACTGGCAAGCTGCGTAATATCGCCATCGCCCAGGG
ATTCACCGACTTCGTGCGCCTGCTGTCAGGTTACGACGCCATTATCCAGGTCGGAGGATCGTTTTTTGTCGATCTCTACG
GCGTGCCGCAGTTTGAACATGCGCTTTGCACGTTTATGGCGAAAAAGCCGCTGTTTATGATTGGTCACAGCGTCGGCCCC
TTCCAGGATGAGCAATTTAACCAACTGGCGAACTACGTTTTTGGTCACTGTGACGCGCTGATCCTGCGCGAATCGGTCAG
CCTTGATCTGATGAAACGCAGCAATATCACCACTGCAAAAGTGGAACATGGCGTCGATACCGCGTGGCTGGTCGATCACC
ACACAGAAGACTTCACCGCCAGCTATGCTGTTCAACACTGGCTGGACGTTGCCGCACAACAGAAAACGGTGGCAATTACC
CTGCGCGAACTGGCACCGTTCGACAAACGTCTCGGCACCACTCAACAAGCGTATGAAAAAGCCTTTGCCGGGGTGGTAAA
TCGCATTCTCGATGAAGGGTATCAGGTGATTGCGCTCTCCACCTGTACGGGCATCGACAGCTATAACAAAGACGACCGCA
TGGTGGCGCTCAACCTGCGCCAGCATATCAGCGATCCTGCCCGTTATCACGTAGTGATGGATGAACTCAACGATCTGGAA
ATGGGCAAAATTCTCGGTGCCTGTGAACTCACCGTCGGTACGCGCCTGCACTCCGCCATTATCTCAATGAACTTTGCTAC
TCCGGCGATTGCCATCAACTATGAACATAAATCCGCCGGGATTATGCAGCAGCTGGGACTACCGGAGATGGCAATTGATA
TCCGTCATTTATTAGACGGCAGCCTGCAAGCGATGGTTGCGGATACCTTAGGCCAGCTTCCGGCGCTGAACGCACGACTT
AGCGAAGCCGTCAGTCGTGAGCGTCAGACGGGAATGCAGATGGTGCAATCTGTGCTTGAGCGCATCGGGGAGGTGAAATG
A

Upstream 100 bases:

>100_bases
CTTGTAGGCCTGATAAGAGGCGTCAGCGTCGCATCAGGCAAAAAGCGGCAACGAACAAAACAAACGCAGCCTCACCTGGC
ATCAAATTTATGAGGAAAAA

Downstream 100 bases:

>100_bases
AGGTCGGCTTCTTTTTACTGAAATTTCCGCTGTCTTCGGAAACCTTCGTCCTCAATCAAATTACCGCGTTTATTGATATG
GGCTTTGAGGTGGAGATTGT

Product: putative pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL
SEAVSRERQTGMQMVQSVLERIGEVK

Sequences:

>Translated_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL
SEAVSRERQTGMQMVQSVLERIGEVK
>Mature_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARL
SEAVSRERQTGMQMVQSVLERIGEVK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=426, Percent_Identity=99.5305164319249, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47301; Mature: 47301

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEECCCCCCCCEE
LQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHCCHHHHHCCCCCEEHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARLSEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC
>Mature Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEECCCCCCCCEE
LQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHCCHHHHHCCCCCEEHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPALNARLSEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]