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The map label for this gene is rutB

Identifier: 218694547

GI number: 218694547

Start: 1180604

End: 1181296

Strand: Reverse

Name: rutB

Synonym: EC55989_1122

Alternate gene names: 218694547

Gene position: 1181296-1180604 (Counterclockwise)

Preceding gene: 218694548

Following gene: 218694546

Centisome position: 22.92

GC content: 54.83

Gene sequence:

>693_bases
ATGACAACCTTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCAAAA
CGCTTATGCCACGCCAGGCGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACATTC
AAACCGCCGTGACCGCAGCGCGAGCGGCAGGGATGCTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTCGAA
GCTGGCGGACCCGGCTCACCGAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTGCAGGGGAA
ATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGAAGC
CGCGCTACAGCGGTTTCTTCAATACACCGCTGGACAGCATTTTGCGCAGCCGTGGAATACGCCATCTGGTTTTCACCGGT
ATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGATGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGACGC
AACACACCAGGCGGGGCCGGAATTTGCACAGAAAGCCGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCGACG
TCGAAACGTTCTGCGACGCGCTTTCTCCCACATCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
TCGATGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTACCTGCG
CTGACTCAGGAGGTGGCATG

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: enzyme of the alternative pyrimidine degradation pathway

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_230_residues
MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_229_residues
TTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEA
GGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGI
ATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=99.1304347826087, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25229; Mature: 25098

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
TTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA