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The map label for this gene is traY

Identifier: 218692899

GI number: 218692899

Start: 89453

End: 89680

Strand: Reverse

Name: traY

Synonym: p1ECUMN_0114

Alternate gene names: 218692899

Gene position: 89680-89453 (Counterclockwise)

Preceding gene: 218692900

Following gene: 218692897

Centisome position: 73.33

GC content: 42.11

Gene sequence:

>228_bases
GTGAGGAGGCGTAACGCGAGAGGCGGGATAAGCAGAACAGTTTCAGTGTATCTCGATGAAGATACAAATAACCGACTTAT
TCGGGCCAAAGATCGTAGCGGTAGAAGTAAAACTATAGAGGTTCAGATCCGTCTCAGAGATCATCTGAAACGGTTTCCTG
ATTTCTATAATGAAGAAATATTCAGGGAGGTGATCGAAGAGAACGAGTCAACATTTAAAGAACTTTAG

Upstream 100 bases:

>100_bases
TAAGTAAATGTTAAATAAGGTGCGCCAATTTTGGCGTGTTTTTTTTCGTCGGTTTCTGTGCAATCATGATTTTATTAGTC
GGGAAACAGAGGTGAATTGA

Downstream 100 bases:

>100_bases
GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTC
CCGTAAATGCCTTGAATCAG

Product: conjugal transfer protein TraY

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 75; Mature: 75

Protein sequence:

>75_residues
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENESTFKEL

Sequences:

>Translated_75_residues
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENESTFKEL
>Mature_75_residues
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVIEENESTFKEL

Specific function: Involved in the conjugation process of bacterial cells for the exchange of plasmid DNA. It is also responsible for conjugal DNA metabolism. TraY is required for strand-specific nicking at oriT, the transfer origin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the traY family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRAY2_ECOLX (P10512)

Other databases:

- EMBL:   X13681
- EMBL:   M19710
- PIR:   C24544
- RefSeq:   YP_003108301.1
- RefSeq:   YP_003829133.1
- RefSeq:   YP_003829258.1
- GeneID:   8319231
- GeneID:   9538060
- GeneID:   9538191
- ProtClustDB:   PRK13740
- GO:   GO:0000746
- InterPro:   IPR008876

Pfam domain/function: PF05509 TraY

EC number: NA

Molecular weight: Translated: 9072; Mature: 9072

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEI
CCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCEEEEEEEEEHHHHHHCCCCCCHHH
FREVIEENESTFKEL
HHHHHHCCHHHHHCC
>Mature Secondary Structure
MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEI
CCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCEEEEEEEEEHHHHHHCCCCCCHHH
FREVIEENESTFKEL
HHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3009392; 2564189