Click here to switch to the map view.

The map label for this gene is lpfB [H]

Identifier: 218691836

GI number: 218691836

Start: 4151807

End: 4152493

Strand: Reverse

Name: lpfB [H]

Synonym: ECED1_4230

Alternate gene names: 218691836

Gene position: 4152493-4151807 (Counterclockwise)

Preceding gene: 218691837

Following gene: 218691835

Centisome position: 79.71

GC content: 41.05

Gene sequence:

>687_bases
ATGAAACGAATTAGCCTTGCCGTTCTGGCGCTTTTAATGACCGCACAACAGTGTTTTGCTGGCGGCGTAGCCCTAAGCAC
GACGCGCGTTATTTATGATGGTGGCAAGAAAGAAGCGTCGCTGACCGTTGAAAACCATAATAAAAATGAAGAGTTTTTGA
TCCAGTCATGGATTGATGATGTGAATGGAAATAAAAAAACGCCGTTTATTATTACACCGCCATTATTCAAACTTGATCCT
GATAAAAATAACGTCTTACGTATTGTTAATATTGAAGATACCTTACCCAAAGATCGTGAATCCGTTTACTGGATTAATGT
AAAAGCGATTCCTGCGAAAAGTGCTGAAAATGAAAATAAAAACGTCTTACAGATAGCCGTTCGCACCCGTTTGAAGTTGT
TCTACCGTCCGAAAGGCTTGCCGGGAAGCAACCTTGAAGCAATGAAACAGTTACGATTTCAGCGTCAAAATAACCAAATT
GTGGTTAATAACGACTCAGCTTATAACCTGACCTTTAATCAGATGTCCATTAATGGACAAAAGATTGAACGGGCGGGAAT
GGTCCTCGCTAAAGGAACGTTAAACATTAACCTTCCAGCCGGTACAGGCAATGCGAACGAAGTGAAATACAGCATTATTA
ATGATTTCGGTGTCGCCGGTGAAATGCTGACGAAGTCGATTAACTGA

Upstream 100 bases:

>100_bases
ACCTGACTTACAACTAATCGATCCAGACGGAACGATTATCTCTCAATGGCAACAGATTATCTGTTGCCATTATTCACACC
AATGACTCAGGGACCGAATC

Downstream 100 bases:

>100_bases
TCTTTTTACAAGGCGCTTTACTATGCATTGGATGCATTTACCTCTTGATCACTACAGAGCGCATTTTTCTTTCTCACTGT
TGGCATTAACTATTGCCAGC

Product: Chaperone protein lpfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGVAGEMLTKSIN

Sequences:

>Translated_228_residues
MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGVAGEMLTKSIN
>Mature_228_residues
MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDP
DKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGVAGEMLTKSIN

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=221, Percent_Identity=37.5565610859729, Blast_Score=167, Evalue=4e-43,
Organism=Escherichia coli, GI1790771, Length=229, Percent_Identity=38.4279475982533, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1786743, Length=219, Percent_Identity=38.8127853881278, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787171, Length=217, Percent_Identity=35.9447004608295, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788428, Length=220, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786333, Length=237, Percent_Identity=32.0675105485232, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=29.4930875576037, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082203, Length=180, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788677, Length=204, Percent_Identity=32.3529411764706, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081806, Length=220, Percent_Identity=30.4545454545455, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25407; Mature: 25407

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
VNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENK
CCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCC
NVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQIVVNNDSAYNLTFNQMSINGQ
CEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCH
KIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGVAGEMLTKSIN
HHHHCCEEEEECEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVENHNKNEEFLIQSWIDD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
VNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESVYWINVKAIPAKSAENENK
CCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCC
NVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQIVVNNDSAYNLTFNQMSINGQ
CEEEEEEEHHEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCH
KIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGVAGEMLTKSIN
HHHHCCEEEEECEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]