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The map label for this gene is mscL

Identifier: 218691577

GI number: 218691577

Start: 3879409

End: 3879822

Strand: Direct

Name: mscL

Synonym: ECED1_3954

Alternate gene names: 218691577

Gene position: 3879409-3879822 (Clockwise)

Preceding gene: 218691576

Following gene: 218691609

Centisome position: 74.47

GC content: 44.44

Gene sequence:

>414_bases
ATGAGCATTATTAAAGAATTTCGCGAATTTGCGATGCGCGGGAACGTGGTGGATTTGGCAGTGGGTGTCATTATCGGAGC
GGCATTCGGGAAGATTGTATCTTCACTGGTTGCCGATATCATCATGCCACCTCTGGGCTTATTAATTGGCGGGATCGACT
TTAAACAGTTTGCTGTCACGCTACGCGAGGCCCAAGGGGATATCCCTGCTGTTGTGATGCATTACGGTGTCTTCATTCAA
AACGTCTTTGATTTTCTGATAGTGGCCTTTGCCATCTTTATGGCGATTAAGCTAATCAACAAACTGAATCGGAAAAAAGA
AGAACCAGCAGCAGCAACACCTGCACCAACTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGA
ATAACCGCTCTTAA

Upstream 100 bases:

>100_bases
TTTCCCTTTGATTATTAAGGATTAATTAAATCCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTC
GGCTTCATAGGGAGAATAAC

Downstream 100 bases:

>100_bases
CAAGCGTCTGAAAGCAGAAGGCCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTTCCCCCGATTGCC
ATGTTTTCCTTTTCGCATGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQ
NVFDFLIVAFAIFMAIKLINKLNRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS

Sequences:

>Translated_137_residues
MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQ
NVFDFLIVAFAIFMAIKLINKLNRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS
>Mature_136_residues
SIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQN
VFDFLIVAFAIFMAIKLINKLNRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=98.5401459854015, Blast_Score=263, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ECO45 (B7MCQ6)

Other databases:

- EMBL:   CU928161
- RefSeq:   YP_002393265.1
- ProteinModelPortal:   B7MCQ6
- SMR:   B7MCQ6
- EnsemblBacteria:   EBESCT00000137509
- GeneID:   7130334
- GenomeReviews:   CU928161_GR
- GeneTree:   EBGT00050000009963
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- BioCyc:   ECOL585035:ECS88_3678-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15072; Mature: 14941

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x249f4dc8)-; HASH(0x243aea9c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVT
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAATPAPTKEE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VLLTEIRDLLKEQNNRS
HHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
SIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAATPAPTKEE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VLLTEIRDLLKEQNNRS
HHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA