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The map label for this gene is rng

Identifier: 218691528

GI number: 218691528

Start: 3828578

End: 3830047

Strand: Reverse

Name: rng

Synonym: ECED1_3896

Alternate gene names: 218691528

Gene position: 3830047-3828578 (Counterclockwise)

Preceding gene: 218691529

Following gene: 218691527

Centisome position: 73.52

GC content: 52.24

Gene sequence:

>1470_bases
ATGACGGCTGAATTGTTGGTAAACGTAACGCCTTCGGAAACGCGAGTGGCGTATATTGATGGCGGTATTCTGCAGGAAAT
TCATATTGAACGTGAGGCGCGACGCGGAATAGTAGGCAATATCTACAAGGGTCGTGTAAGTCGTGTACTTCCGGGTATGC
AGGCGGCTTTTGTAGATATTGGGCTGGATAAAGCCGCGTTTCTTCATGCATCTGACATCATGCCGCACACTGAATGTGTG
GCGGGTGAAGAACAAAAGCAATTCACGGTGCGCGACATCTCGGAACTGGTCCGTCAGGGGCAAGATCTGATGGTGCAGGT
GGTGAAAGATCCGCTTGGCACTAAAGGTGCGCGCCTGACCACCGATATCACGCTACCTTCTCGCTATCTGGTGTTTATGC
CAGGGGCTTCTCACGTTGGGGTATCCCAACGAATTGAAAGCGAATCAGAACGTGAACGCCTGAAAAAAGTGGTCGCAGAG
TATTGCGACGAGCAGGGCGGGTTTATCATCCGTACTGCAGCGGAAGGGGTTGGCGAGGCTGAACTGGCCTCCGATGCCGC
TTATCTGAAACGCGTCTGGACCAAAGTGATGGAGCGTAAAAAACGCCCGCAGACCCGTTATCAGCTGTACGGAGAACTGG
CGCTGGCGCAGCGTGTTCTGCGTGATTTCGCCGACGCCGAACTCGACCGCATTCGCGTTGACTCACGCCTGACTTACGAA
GCATTGCTGGAGTTTACCTCGGAGTACATTCCCGAGATGACCAGCAAGCTGGAGCATTACACCGGACGCCAGCCGATTTT
CGATCTCTTTGATGTCGAAAACGAAATTCAGCGAGCGCTGGAACGCAAAGTAGAACTGAAATCCGGTGGTTATCTGATTA
TCGACCAGACCGAAGCGATGACCACCGTGGACATCAATACCGGAGCGTTTGTCGGTCATCGCAATCTGGACGACACCATT
TTCAATACCAATATTGAAGCGACGCAGGCTATCGCTCGCCAGTTACGGTTGCGCAATCTGGGCGGGATTATCATTATTGA
TTTCATCGATATGAATAATGAAGATCACCGTCGCCGCGTGCTGCACTCGCTGGAGCAGGCGCTGAGCAAAGATCGGGTGA
AAACCAGCGTTAATGGTTTTTCGGCGCTGGGGCTGGTGGAGATGACGCGTAAACGCACCCGCGAAAGCATTGAGCACGTA
CTGTGTAACGAATGCCCAACCTGCCACGGTCGCGGAACGGTGAAAACCGTGGAAACGGTATGCTATGAAATCATGCGCGA
GATTGTTCGTGTCCACCATGCTTACGACTCCGACCGTTTCCTGGTCTATGCTTCTCCGGCAGTAGCCGAAGCCTTGAAAG
GCGAAGAGTCACACTCGCTGGCGGAAGTGGAAATTTTCGTTGGCAAACAGGTTAAAGTACAAATTGAACCGCTCTATAAC
CAGGAGCAGTTTGACGTCGTAATGATGTAA

Upstream 100 bases:

>100_bases
AAGATAAATGGCAGCTTTCACGCCGTAGTCGGCTTACCGCTGGTTGAAACGTATGAATTATTAAGTAATTTTAACGCACT
GCGTGAGAAAAGGGATAAAC

Downstream 100 bases:

>100_bases
ACAGATGCTGGGCCGCCATCCGGCAAAGGGTTTTTGAGTCACATTTTTAGCAGACAAGGAGTGACGGGTGAGGCGATTGC
CGGGGATTTTACTGCTTACT

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGEEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
ALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHSLAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM

Sequences:

>Translated_489_residues
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGEEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
ALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHV
LCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHSLAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM
>Mature_488_residues
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVA
GEEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEY
CDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYEA
LLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIF
NTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVL
CNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHSLAEVEIFVGKQVKVQIEPLYNQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=100, Blast_Score=1004, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=436, Percent_Identity=35.7798165137615, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNG_ECO57 (P0A9J2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91143
- RefSeq:   NP_289814.2
- RefSeq:   NP_312146.1
- ProteinModelPortal:   P0A9J2
- SMR:   P0A9J2
- MINT:   MINT-1221274
- EnsemblBacteria:   EBESCT00000025524
- EnsemblBacteria:   EBESCT00000059580
- GeneID:   916034
- GeneID:   958681
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4605
- KEGG:   ecs:ECs4119
- GeneTree:   EBGT00050000008748
- HOGENOM:   HBG756312
- OMA:   HQRQVLR
- ProtClustDB:   PRK11712
- BioCyc:   ECOL83334:ECS4119-MONOMER
- GO:   GO:0005737
- GO:   GO:0005856
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316
- TIGRFAMs:   TIGR00757

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55365; Mature: 55234

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCC
GLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHCCCHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEYCDEQGGFIIRTAAEGVGEA
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
ELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEECCCCHHHH
ALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHSLAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCEEEEC
>Mature Secondary Structure 
TAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCC
GLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHCCCHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEYCDEQGGFIIRTAAEGVGEA
EEEECCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
ELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQRVLRDFADAELDRIRVDSRLTYE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHEECCCCHHHH
ALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEIQRALERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRV
EEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
LHSLEQALSKDRVKTSVNGFSALGLVEMTRKRTRESIEHVLCNECPTCHGRGTVKTVETV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEIMREIVRVHHAYDSDRFLVYASPAVAEALKGEESHSLAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796