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The map label for this gene is insB [H]

Identifier: 218691092

GI number: 218691092

Start: 3376706

End: 3377023

Strand: Reverse

Name: insB [H]

Synonym: ECED1_3432

Alternate gene names: 218691092

Gene position: 3377023-3376706 (Counterclockwise)

Preceding gene: 218691093

Following gene: 218691089

Centisome position: 64.82

GC content: 56.6

Gene sequence:

>318_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACTGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGGACGGATGGCTGGCCGCTGTATGA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAGACAACATC
TGGCAAGGCTGGGACGGAAG

Product: transposase ORF B (fragment), IS1

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 105; Mature: 104

Protein sequence:

>105_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWDGWLAAV

Sequences:

>Translated_105_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWDGWLAAV
>Mature_104_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWDGWLAAV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=103, Percent_Identity=83.495145631068, Blast_Score=182, Evalue=3e-48,
Organism=Escherichia coli, GI1788203, Length=103, Percent_Identity=83.495145631068, Blast_Score=182, Evalue=3e-48,
Organism=Escherichia coli, GI1786203, Length=103, Percent_Identity=83.495145631068, Blast_Score=182, Evalue=3e-48,
Organism=Escherichia coli, GI1787223, Length=103, Percent_Identity=81.5533980582524, Blast_Score=180, Evalue=2e-47,
Organism=Escherichia coli, GI1786470, Length=103, Percent_Identity=81.5533980582524, Blast_Score=179, Evalue=5e-47,
Organism=Escherichia coli, GI1786459, Length=103, Percent_Identity=81.5533980582524, Blast_Score=179, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 12172; Mature: 12041

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWDGWLAAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWDGWLAAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]