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The map label for this gene is ygcM [H]

Identifier: 218690885

GI number: 218690885

Start: 3148011

End: 3148376

Strand: Direct

Name: ygcM [H]

Synonym: ECED1_3214

Alternate gene names: 218690885

Gene position: 3148011-3148376 (Clockwise)

Preceding gene: 218690880

Following gene: 218690886

Centisome position: 60.43

GC content: 49.18

Gene sequence:

>366_bases
ATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTGCCGCACGTCCCACAAGGGCATAAATG
TGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCG
ATTTCGCTGAACTGAAAGCGGCGTTTAAACCAACTTATGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTG
GAAAACCCAACCAGCGAAGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGAT
GGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGA

Upstream 100 bases:

>100_bases
AGATAAATCAGCGATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTT
TATCGTCATGTAGAGAAATT

Downstream 100 bases:

>100_bases
TAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATG
GAAGACGACTGCGACATTAT

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE

Sequences:

>Translated_121_residues
MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE
>Mature_121_residues
MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGL
ENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRGE

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=121, Percent_Identity=99.1735537190083, Blast_Score=253, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 13772; Mature: 13772

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCEEEEHHHHHH
AFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRG
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
E
C
>Mature Secondary Structure
MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEVDPHTGWIIDFAELKA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCEEEEHHHHHH
AFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLLSAVMVKETCTAGCIYRG
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]