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The map label for this gene is mexA [H]

Identifier: 218689283

GI number: 218689283

Start: 1472518

End: 1473672

Strand: Direct

Name: mexA [H]

Synonym: ECED1_1501

Alternate gene names: 218689283

Gene position: 1472518-1473672 (Clockwise)

Preceding gene: 218689282

Following gene: 218689284

Centisome position: 28.27

GC content: 50.91

Gene sequence:

>1155_bases
ATGAGGAGGGTATTTCTCACCCTGGAGAAAATAATGAAGTATATAGCAACATCTGTAGTGGCAATGCTGCTCTTATCGGG
TTGTGATAATACGCAAAGTAACAATTCATCCCCGTCAGAAACAGAAGTCGGCGTTGTTACGCTCAAATCTCAACCGGTTT
CGGTAGTCAGTGAATTAACCGGACGCACCAGTGCTGCGCTCAGTGCTGAAGTACGTCCGCAGGTTGGGGGAATTATCCAG
AAACGCTTATTTAAGGAAGGTGATCTGGTCAAGGCTGGACAGCCTCTCTACCAGATTGATGCGGCCAGTTATCAGGCTGC
ATGGAATGAAGCCCGGGCAGCATTACAACAAGCACAGGCACTGGTAAAAGCCGATTGCCAGAAAGCGCAGCGTTATGCCC
GACTGGTGAAAGAGAATGGTGTTTCACAACAGGATGCTGATGATGCTCAGTCTACCTGTGCACAAGATAAAGCCAGTGTA
GAGGCGAAAAAAGCCGCACTGGAAACTGCGCGCATTAATCTTGACTGGACCACGGTAACCGCACCGATTTCGGGGCGTAT
TGGTATTTCGTCGGTAACCCTTGGCGCACTGGTGACCGCGTCGCAGGATACAGCGTTAACGACTATTCGTGGTCTGGATA
CAATGTATGTCGACCTCACTCGCTCCAGTGTCGATTTATTACGTCTGCGTAAACAGTCACTGGCGACCAACAGTGACACC
ATGAGCGTCTCACTTATTCTGGAAGATGGCACAACCTACAGCGAAAAAGGGCGTCTGGAACTCACCGAAGTCGCGGTGGA
TGAGTCTACCGGTTCGGTGACATTACGGGCAATTTTCCCCAATCCACAACAGCAGTTATTACCGGGAATGTTTGTTCGCG
CTCGTGTCGATGAAGGCGTGATGGAAGACGCTATTCTCGCGCCGCAACAGGGCGTCACGCGCGATGCTAAAGGCAATGCA
ACTGCGCTGGTGGTCAATAAAGACAATAAAGTAGAGCAGCGAACGCTCGAAACGGGAGAAACGTATGGTGATAAATGGCT
GGTGCTGAACGGCCTGCACAACGGCGACCGACTGATTGTTGAAGGTTCTGCCAAAGTCACTTCAGGGCAGACCGTCAAGG
CTGTTGAAGTTCAGGCTAATGGAGGCAACGCCTGA

Upstream 100 bases:

>100_bases
ATAGCGGGGGATATTAATCCCCCTTATTTCATTGCGAAATTCATTTCGCGATGCGGGTTCGTTATAACTGAATATCCCTG
ACATATCCTCATTAATTTCA

Downstream 100 bases:

>100_bases
TGTTTTCGCGCTTTTTTGTTCGCCGCCCGGTCTTTGCCTGGGTTATCGCCATTTTGATTATGTTGGCGGGGATTCTGGCT
ATTCGCACATTGCCCGTCGC

Product: putative efflux protein, membrane fusion (MFP) family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTLGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRTLETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA

Sequences:

>Translated_384_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTLGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRTLETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA
>Mature_384_residues
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELTGRTSAALSAEVRPQVGGIIQ
KRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASV
EAKKAALETARINLDWTTVTAPISGRIGISSVTLGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVMEDAILAPQQGVTRDAKGNA
TALVVNKDNKVEQRTLETGETYGDKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=381, Percent_Identity=49.8687664041995, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=48.1382978723404, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=46.9101123595506, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=28.3076923076923, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87081796, Length=328, Percent_Identity=24.6951219512195, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41116; Mature: 41116

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQA
CCCHHHHEECCCCHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
LVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEE
APISGRIGISSVTLGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
ECCCCCCCCCCEEEEHEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGV
EEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCH
MEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDKWLVLNGLHNGDRLIV
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
EGSAKVTSGQTVKAVEVQANGGNA
ECCCEECCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MRRVFLTLEKIMKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSVVSELT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHC
GRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQA
CCCHHHHEECCCCHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
LVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETARINLDWTTVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEE
APISGRIGISSVTLGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDT
ECCCCCCCCCCEEEEHEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
MSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGV
EEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHCCCCEEEECCCCCH
MEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDKWLVLNGLHNGDRLIV
HHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
EGSAKVTSGQTVKAVEVQANGGNA
ECCCEECCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]