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The map label for this gene is proP [H]

Identifier: 218551389

GI number: 218551389

Start: 4275863

End: 4277365

Strand: Direct

Name: proP [H]

Synonym: EFER_4170

Alternate gene names: 218551389

Gene position: 4275863-4277365 (Clockwise)

Preceding gene: 218551388

Following gene: 218551400

Centisome position: 93.18

GC content: 49.7

Gene sequence:

>1503_bases
ATGCTGAAAAAGAAAAAAGTAAAACCCATTACGCTTCGTGATGTCACGATTATCGATGACGGAAAATTGCGAAAAGCCAT
TACCGCAGCCTCGCTGGGTAACGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAG
TGTTCTTTCCGGGAGCCGACCCCAGCGTGCAGATGGTTGCCGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCG
CTTGGCGGACTTTTCTTCGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTTGCGATCACCATTGTGATTATGTC
GGTCAGTACTTTCTGTATCGGTTTAATTCCTTCCTATGCCTCCATTGGTATTTGGGCGCCGATCCTGTTGCTGCTCTGTA
AGATGGCGCAAGGTTTCTCCGTCGGTGGCGAATATACCGGGGCATCGATATTTGTTGCGGAATACTCTCCTGACCGTAAA
CGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCCATTGCCGGGTTTGTGCTGGGGGCAGGCGTGGTGGTCCTTATTTC
GACCATTGTTGGCGAGGAGAATTTCCTCGACTGGGGCTGGCGTATTCCGTTCTTTATCGCTCTGCCGTTAGGGATTATTG
GCCTTTACTTGCGTCATGCGCTGGAAGAGACACCCGCGTTCCAGCAACATGTTGAGAAACTGGAGCAGGGCGACCGTGAA
GGTTTGCAGGATGGCCCAAAAGTTTCATTTAAAGAGATCGCCACTAAACACTGGCGTAGTTTGCTGGCATGTATTGGTCT
GGTGATCGCGACGAACGTCACTTACTACATGTTGCTGACTTACATGCCGAGTTATCTTTCGCATAACCTGCATTACTCGG
AAGATCACGGCGTGTTGATTATCATCGCTATTATGATCGGTATGCTGTTTGTGCAGCCAGTGATGGGGCTGCTTAGCGAC
CGGTTTGGCCGTCGTCCGTTTGTTATTCTCGGCAGTATTGCGCTGTTTGTGCTGGCGATTCCGGCGTTTATTTTGATTAA
TAGTAATATTATCGGCCTGATTTTTGCCGGGCTGTTGATGCTGGCGGTGATCCTCAACTGTTTTACCGGGGTCATGGCCT
CCACGCTTCCGGCGATGTTCCCGACGCATATTCGCTACAGTGCACTGGCAGCTGCGTTTAATATTTCGGTATTGATTGCT
GGTTTGACACCAACCGCAGCAGCCTGGTTGGTGGAAAGCTCGCATAATTTGATGATGCCAGCGTACTACCTGATGGTGGT
GGCGGTGATTGGTTTAATTACTGGCATTACCATGAAAGAGACGGCGAATCGTCCGCTGAAAGGTGCGACACCAGCGGCGT
CAGACTTGCAGGAAGCGAAGGAAATTCTTGGTGAGCATTACGATAATATTGAGCAGAAAATCGATGATATCGATCTCGAA
ATTGCCGATTTGCAGGCGAAACGTTCTCGCCTGGTGCAGCAACATCCGCGCATAGTGGAGTAA

Upstream 100 bases:

>100_bases
GTTTCAACGATTGCACCCTGCAATTCCCGCTTACCGTTAGCGGACGCCACGCTGAGTCATTTGGCGTGTGCGTACTCGGG
CTTAGTCTGCGAGGACAGCT

Downstream 100 bases:

>100_bases
CCTGACGCGATGCTGCACGCTTAAGGTTCACTTAATCTCCAGGATGCATACTCTCCTCCAGTTCAACGGAGGAGAGTGTC
ATGAAAAACCGTGTTTATGA

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLKKKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSVSTFCIGLIPSYASIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVEKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLACIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVILGSIALFVLAIPAFILINSNIIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTAAAWLVESSHNLMMPAYYLMVVAVIGLITGITMKETANRPLKGATPAASDLQEAKEILGEHYDNIEQKIDDIDLE
IADLQAKRSRLVQQHPRIVE

Sequences:

>Translated_500_residues
MLKKKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSVSTFCIGLIPSYASIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVEKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLACIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVILGSIALFVLAIPAFILINSNIIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTAAAWLVESSHNLMMPAYYLMVVAVIGLITGITMKETANRPLKGATPAASDLQEAKEILGEHYDNIEQKIDDIDLE
IADLQAKRSRLVQQHPRIVE
>Mature_500_residues
MLKKKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRP
LGGLFFGMLGDKYGRQKILAITIVIMSVSTFCIGLIPSYASIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRK
RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVEKLEQGDRE
GLQDGPKVSFKEIATKHWRSLLACIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSD
RFGRRPFVILGSIALFVLAIPAFILINSNIIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIA
GLTPTAAAWLVESSHNLMMPAYYLMVVAVIGLITGITMKETANRPLKGATPAASDLQEAKEILGEHYDNIEQKIDDIDLE
IADLQAKRSRLVQQHPRIVE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=500, Percent_Identity=95.6, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=445, Percent_Identity=32.3595505617978, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789941, Length=452, Percent_Identity=33.1858407079646, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788942, Length=440, Percent_Identity=29.5454545454545, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082231, Length=487, Percent_Identity=22.9979466119097, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87082404, Length=366, Percent_Identity=26.2295081967213, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323512, Length=551, Percent_Identity=23.5934664246824, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54704; Mature: 54704

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGAD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSVSTFCIGLIPSYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVEKLEQGDRE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLACIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMIGMLFVQPVMGLLSDRFGRRPFVILGSIALFVLAIPAFILINSNIIGLIFAGLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIAGLTPTAAAWLVESSHNLMMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCCCCCHH
AYYLMVVAVIGLITGITMKETANRPLKGATPAASDLQEAKEILGEHYDNIEQKIDDIDLE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IADLQAKRSRLVQQHPRIVE
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKKKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGAD
CCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSVSTFCIGLIPSYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIGIWAPILLLLCKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
GVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVEKLEQGDRE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GLQDGPKVSFKEIATKHWRSLLACIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IIAIMIGMLFVQPVMGLLSDRFGRRPFVILGSIALFVLAIPAFILINSNIIGLIFAGLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLIAGLTPTAAAWLVESSHNLMMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCCCCCHH
AYYLMVVAVIGLITGITMKETANRPLKGATPAASDLQEAKEILGEHYDNIEQKIDDIDLE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IADLQAKRSRLVQQHPRIVE
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]