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The map label for this gene is mdtL [H]

Identifier: 218551241

GI number: 218551241

Start: 4111444

End: 4112619

Strand: Direct

Name: mdtL [H]

Synonym: EFER_4006

Alternate gene names: 218551241

Gene position: 4111444-4112619 (Clockwise)

Preceding gene: 218551240

Following gene: 218551242

Centisome position: 89.6

GC content: 52.98

Gene sequence:

>1176_bases
ATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACC
GCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCTGTTTATCTGGCAGGTATGGCAGCAG
CGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCTGTCGCCATACCCGGCGCGGCGCTATTTATTATT
GCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGACGATTTCTACAGGGGCTGGGCGCAGGCTG
TTGTTACGTGGTGGCGTTCGCCATTTTACGTGACACACTGGACGATCGACGTCGGGCAAAAGTGCTGTCGTTACTCAACG
GCATTACCTGCATCATTCCGGTGTTAGCCCCGGTACTCGGACACCTGATTATGCTTAAGTTCCCGTGGCAAAGTCTGTTC
TGGGCGATGGCAACGATGGGCATCGCAGTACTGATGTTGTCTTTGTTTATTTTGAAAGAAACGCGCCCCGCAGCCCCCGC
CGCTTCGGACAAACCACGAGAGAATAGGGAGTCGCTGCTTAACCGGTTTTTCCTCAGCCGTGTGGTAATCACCACCCTCA
GCGTTTCGGTAATCCTGACCTTCGTGAATACGTCGCCGGTATTGCTGATGGAAATCATGGGCTTTGAGCGCGGAGAATAC
GCCACCATAATGGCGTTGACCGCAGGTGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCACTGGGGATTTTTAAGCC
ACGTACGTTGATGATCACCTCGCAGGTGTTATTTCTGGCGGCGGGTATCACTCTTGCCGTTTCGCCCTCCCATGCGATCT
CTTTGTTTGGTATCACGCTGATTTGCGCTGGGTTCTCGATAGGTTTTGGCGTGGCGATGAGTCAGGCGTTAGGGCCGTTT
TCATTACGTGCGGGTGTTGCCAGCTCGACCTTAGGTATTGCACAGGTTTGCGGATCGTCACTGTGGATTTGGCTGGCGGC
GGTGGTTGGTATCGGCGCATGGAATATGCTGATCGGGATTCTGATTGCCTGTAGCATAGTGAGCCTGTTGCTGATTATGT
TCGTCACGCCTGGACGCCCCGTTGCCGCTCATGAAGAAATCCATCACCACGCTTGA

Upstream 100 bases:

>100_bases
CTTGCCTGTCAGGAAATCACTTATGTCCAAATCGCAACTCGCCTGATTCTGCTTCACCACGTATGCTTTGCGTCACCTTA
CTATCAGGACGCTTTAGCCC

Downstream 100 bases:

>100_bases
TCTCAATTTGCTGCTCTGTCTGCAACTGCTGATGCAGGAACGCAGCGTGACTAAAGCGGCGAAGCGGATGAACGTGACCC
CTTCGGCGGTGAGTAAGTCG

Product: multidrug efflux system protein MdtL

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WAMATMGIAVLMLSLFILKETRPAAPAASDKPRENRESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAISLFGITLICAGFSIGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVTPGRPVAAHEEIHHHA

Sequences:

>Translated_391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WAMATMGIAVLMLSLFILKETRPAAPAASDKPRENRESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAISLFGITLICAGFSIGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVTPGRPVAAHEEIHHHA
>Mature_390_residues
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIA
SVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFW
AMATMGIAVLMLSLFILKETRPAAPAASDKPRENRESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEYA
TIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAISLFGITLICAGFSIGFGVAMSQALGPFS
LRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVTPGRPVAAHEEIHHHA

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=98.7212276214834, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1788509, Length=229, Percent_Identity=30.5676855895196, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994889, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790794, Length=227, Percent_Identity=29.0748898678414, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=25.9475218658892, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787065, Length=233, Percent_Identity=23.6051502145923, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081738, Length=176, Percent_Identity=30.1136363636364, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41587; Mature: 41456

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMATMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKPRENRESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAISLFGITL
HEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ICAGFSIGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFVTPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMATMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKPRENRESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAISLFGITL
HEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ICAGFSIGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFVTPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA