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The map label for this gene is mdeA [H]

Identifier: 218550818

GI number: 218550818

Start: 3612957

End: 3614168

Strand: Direct

Name: mdeA [H]

Synonym: EFER_3533

Alternate gene names: 218550818

Gene position: 3612957-3614168 (Clockwise)

Preceding gene: 218550816

Following gene: 218550820

Centisome position: 78.74

GC content: 45.87

Gene sequence:

>1212_bases
ATGACAGGTGAGCAGATGAACCACAATCAAAATTATGGAATGAATACCCGAATCGTCCATGCTGGGCAGCAGCCTGATCC
CTCAACAGGCGCTTTGAGTATGCCTATTTTTCAGACGTCTACTTTTGTCTTTGATAGTGCCGAACAAGGAGCTGCTCGTT
TTGCGCTGGAAGAGCCAGGCTACATTTATACGCGTTTAGGCAACCCAACAGTCGATGCCCTGGAGAAAAAACTCGCAGTA
TTAGAACAGGGAGAAGCGGCACTTGCGACTGCATCAGGAATTTCAGCAATTACCACAACGTTGCTGACTTTATGCCAGCA
AGGGGATCATATTATTTCCGCCAGTGCTATATACGGTTGTACTCATGCGTTTTTACAGCATAGCATTCCCAAATTTGGTA
TTAATGTCAGTTTTGTTAATGCCTCAAAACCAGAGGAAATTGTTGCAGCACTACGCCAGGAAACGAAAGTCATTTATATT
GAAACACCGGCTAATCCGACACTGTCTTTGGTGGATATTGCGGCTGTTGCTGATATTGCCCATCAAAGTGGGGTTATATT
AATTGTCGATAATACTTTTATGTCACCTTATTGCCAGCAGCCACTAACCCTGGGGGCAGATATTGTCATTCATAGTTTAA
CAAAATATATCAATGGTCATGGTGATGTTATTGGTGGTGTTATTATCGGCAAGCAGGAGTTTATCGATCAGGCTCGATTT
GTCGGTCTGAAGGATATTACCGGTGGATGCCTGAGTCCTTTCGACGCCTGGCTAACGTTACGTGGGGTAAAAACCCTGGG
AATACGTATGGAGCGCCATTGTCAGAATGCGTTGAGAATTGCCCATTACCTTGAGTCTCATCCGGCAATTACTCAGGTCT
GGTATCCGGGTCTACCTTCCCACCCACAATATATATTGGGCAAAAAACAGATGAGTCTTCCGGGAGGCGTAATCAGCTTT
GAAATCGCCGGAGGTCTTGAGACTGGCAGACGTATGATCAACTCTGTAGAATTATGCCTCCTCGCAGTCAGCCTCGGTGA
TACCGAAACCCTCATTCAGCATCCTGCGTCTATGACACATTCGCCTTTTGCACCAGAGGAACGGCTTGAAGCAGGTATTA
CCGATGGCCTTATTCGACTGTCAGTAGGTCTGGAGGATCCCGAAGATATCATTAACGACCTTGATCAAGCCATCAGAAAG
GCAACTCACTGA

Upstream 100 bases:

>100_bases
CTGACAGCTGGTTGAAAATAATAAGCATTAACTCACTGATTAAAAACAAATACTCGTCTGGCACAGAGCTTGCTTAGCCA
GTAAGACCTTTTTATTTTCG

Downstream 100 bases:

>100_bases
ATATTAGTCAATGAGGTTAATATATTCGTCATACTTCGAGTTGCAGGACAAGCGAGAGAATCCCCAGAAGCTTGACTTCA
GTAAGTGGCAGGGATGAGTA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTGEQMNHNQNYGMNTRIVHAGQQPDPSTGALSMPIFQTSTFVFDSAEQGAARFALEEPGYIYTRLGNPTVDALEKKLAV
LEQGEAALATASGISAITTTLLTLCQQGDHIISASAIYGCTHAFLQHSIPKFGINVSFVNASKPEEIVAALRQETKVIYI
ETPANPTLSLVDIAAVADIAHQSGVILIVDNTFMSPYCQQPLTLGADIVIHSLTKYINGHGDVIGGVIIGKQEFIDQARF
VGLKDITGGCLSPFDAWLTLRGVKTLGIRMERHCQNALRIAHYLESHPAITQVWYPGLPSHPQYILGKKQMSLPGGVISF
EIAGGLETGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPFAPEERLEAGITDGLIRLSVGLEDPEDIINDLDQAIRK
ATH

Sequences:

>Translated_403_residues
MTGEQMNHNQNYGMNTRIVHAGQQPDPSTGALSMPIFQTSTFVFDSAEQGAARFALEEPGYIYTRLGNPTVDALEKKLAV
LEQGEAALATASGISAITTTLLTLCQQGDHIISASAIYGCTHAFLQHSIPKFGINVSFVNASKPEEIVAALRQETKVIYI
ETPANPTLSLVDIAAVADIAHQSGVILIVDNTFMSPYCQQPLTLGADIVIHSLTKYINGHGDVIGGVIIGKQEFIDQARF
VGLKDITGGCLSPFDAWLTLRGVKTLGIRMERHCQNALRIAHYLESHPAITQVWYPGLPSHPQYILGKKQMSLPGGVISF
EIAGGLETGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPFAPEERLEAGITDGLIRLSVGLEDPEDIINDLDQAIRK
ATH
>Mature_402_residues
TGEQMNHNQNYGMNTRIVHAGQQPDPSTGALSMPIFQTSTFVFDSAEQGAARFALEEPGYIYTRLGNPTVDALEKKLAVL
EQGEAALATASGISAITTTLLTLCQQGDHIISASAIYGCTHAFLQHSIPKFGINVSFVNASKPEEIVAALRQETKVIYIE
TPANPTLSLVDIAAVADIAHQSGVILIVDNTFMSPYCQQPLTLGADIVIHSLTKYINGHGDVIGGVIIGKQEFIDQARFV
GLKDITGGCLSPFDAWLTLRGVKTLGIRMERHCQNALRIAHYLESHPAITQVWYPGLPSHPQYILGKKQMSLPGGVISFE
IAGGLETGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPFAPEERLEAGITDGLIRLSVGLEDPEDIINDLDQAIRKA
TH

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=397, Percent_Identity=43.8287153652393, Blast_Score=291, Evalue=8e-79,
Organism=Homo sapiens, GI299473759, Length=397, Percent_Identity=40.3022670025189, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI25453487, Length=396, Percent_Identity=37.6262626262626, Blast_Score=218, Evalue=6e-57,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=37.984496124031, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=27.4111675126904, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=41.6879795396419, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71980708, Length=394, Percent_Identity=38.3248730964467, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17560056, Length=391, Percent_Identity=41.9437340153453, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319307, Length=395, Percent_Identity=40.7594936708861, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=35.5658198614319, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321254, Length=412, Percent_Identity=32.7669902912621, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=345, Percent_Identity=27.2463768115942, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=401, Percent_Identity=26.9326683291771, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322590, Length=256, Percent_Identity=27.34375, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922554, Length=397, Percent_Identity=41.0579345088161, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43530; Mature: 43399

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGEQMNHNQNYGMNTRIVHAGQQPDPSTGALSMPIFQTSTFVFDSAEQGAARFALEEPG
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCEEECCEEEEECCCCCCCEEEECCCC
YIYTRLGNPTVDALEKKLAVLEQGEAALATASGISAITTTLLTLCQQGDHIISASAIYGC
EEEEECCCCCHHHHHHHHHHHHCCCHHEEHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHH
THAFLQHSIPKFGINVSFVNASKPEEIVAALRQETKVIYIETPANPTLSLVDIAAVADIA
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHH
HQSGVILIVDNTFMSPYCQQPLTLGADIVIHSLTKYINGHGDVIGGVIIGKQEFIDQARF
HCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
VGLKDITGGCLSPFDAWLTLRGVKTLGIRMERHCQNALRIAHYLESHPAITQVWYPGLPS
CCCHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
HPQYILGKKQMSLPGGVISFEIAGGLETGRRMINSVELCLLAVSLGDTETLIQHPASMTH
CCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCHHHHHHCCCCCCC
SPFAPEERLEAGITDGLIRLSVGLEDPEDIINDLDQAIRKATH
CCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGEQMNHNQNYGMNTRIVHAGQQPDPSTGALSMPIFQTSTFVFDSAEQGAARFALEEPG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCEEECCEEEEECCCCCCCEEEECCCC
YIYTRLGNPTVDALEKKLAVLEQGEAALATASGISAITTTLLTLCQQGDHIISASAIYGC
EEEEECCCCCHHHHHHHHHHHHCCCHHEEHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHH
THAFLQHSIPKFGINVSFVNASKPEEIVAALRQETKVIYIETPANPTLSLVDIAAVADIA
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHH
HQSGVILIVDNTFMSPYCQQPLTLGADIVIHSLTKYINGHGDVIGGVIIGKQEFIDQARF
HCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
VGLKDITGGCLSPFDAWLTLRGVKTLGIRMERHCQNALRIAHYLESHPAITQVWYPGLPS
CCCHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
HPQYILGKKQMSLPGGVISFEIAGGLETGRRMINSVELCLLAVSLGDTETLIQHPASMTH
CCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCHHHHHHCCCCCCC
SPFAPEERLEAGITDGLIRLSVGLEDPEDIINDLDQAIRKATH
CCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]