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The map label for this gene is yjjN [H]

Identifier: 218550266

GI number: 218550266

Start: 3026108

End: 3027124

Strand: Direct

Name: yjjN [H]

Synonym: EFER_2958

Alternate gene names: 218550266

Gene position: 3026108-3027124 (Clockwise)

Preceding gene: 218550265

Following gene: 218550267

Centisome position: 65.95

GC content: 47.39

Gene sequence:

>1017_bases
ATGAAAACATTAATTTGTCAGCAGCCTGGCGTTATGGAATATGTGGAAAAGGATATTCCCACATCAGCAGATAATGAAGT
GCTGTTAAAAATCAAAGCTGTGGGTATTTGTGGTACTGATATTCACGCTTTTGCCGGCAGACAGCCTTTTTTTAGCTACC
CACGTGTATTAGGTCATGAAATATGCGCCGAAGCGATTTCGCGAGGCAGTCAGTGCCAAACAGCACAGCCAGGCCAGCGC
TATTCCGTCATCCCATGCATTCCGTGTGGCGAGTGCGCAGCCTGTCGGGAAGAGAAAACGAACTGCTGCGAACGTGTTTC
GCTGTATGGCGTGCATCAGGATGGGGGTTTTAGTGAATACCTTGCGGTACGTGAAGACAACCTTGTGCCGCTCCCTGACG
AGGTAAGCGACAGCGCCGGAGCATTGGTTGAATGTTTCGCCATTGGTGCACATGCCGTTCGTCGGGCTGAGATCAAGGCT
GAACAAAACGTACTGGTGATTGGCGCCGGGCCAATCGGTCTGGCTACCGCAGCCATCGCCAGGGCTAAAGGGGCGCATGT
TGTTGTTGCTGATATTGATTGTCAACGTCGCCAGCACGTTGTGGATCATCTGGCTATTAATGCCTTCGATCCCACACAGG
AAGATTTTATTGTCGCGCTTCGTGAGGCCTTTGGAGGCGAACTGGCTTGCGTAGTACTGGATGCGACGGGAAATAAAGCT
TCAATGAGTCAGGATGTAAATCTTATTCGTCATGGCGGCAAAATTGTTTTCATCGGTTTGTATATTGGTGAACTCGTTAT
TGACGATCCGACCTTCCATAAAAAAGAGACAACTTTACTCAGCAGCCGCAATGCCACACGGGAAGATTTTGCGTTGGTGA
TTGAACTGATGCGCAGCAATAAAATTCACGAAAATTTAATGAAAAACCAGGAGTTCGATTTCTTTAGTGTTGGCGAAGAT
TACCAACGTAACGTTGTAGAAAATAAAAATATGGTCAAGGGTGTGATCACTTTTTAA

Upstream 100 bases:

>100_bases
ACCATCAGCCATTTATAAATGAAATAAAAATCGCATTTGCGAATATAAAAAACAAAGGCATCAAGGCCACCATCAGCCAA
TTATTATCGGATGAGCAGAA

Downstream 100 bases:

>100_bases
GGGAACAAATATGACAACTGTTTATGTCAGTGAAGATAATTTAAAATCACTGGTGCACCACAAATTACATACCGCCGGGC
TGGATCCTGACACTGCACAA

Product: L-threonine 3-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKTLICQQPGVMEYVEKDIPTSADNEVLLKIKAVGICGTDIHAFAGRQPFFSYPRVLGHEICAEAISRGSQCQTAQPGQR
YSVIPCIPCGECAACREEKTNCCERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINAFDPTQEDFIVALREAFGGELACVVLDATGNKA
SMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFALVIELMRSNKIHENLMKNQEFDFFSVGED
YQRNVVENKNMVKGVITF

Sequences:

>Translated_338_residues
MKTLICQQPGVMEYVEKDIPTSADNEVLLKIKAVGICGTDIHAFAGRQPFFSYPRVLGHEICAEAISRGSQCQTAQPGQR
YSVIPCIPCGECAACREEKTNCCERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINAFDPTQEDFIVALREAFGGELACVVLDATGNKA
SMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFALVIELMRSNKIHENLMKNQEFDFFSVGED
YQRNVVENKNMVKGVITF
>Mature_338_residues
MKTLICQQPGVMEYVEKDIPTSADNEVLLKIKAVGICGTDIHAFAGRQPFFSYPRVLGHEICAEAISRGSQCQTAQPGQR
YSVIPCIPCGECAACREEKTNCCERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINAFDPTQEDFIVALREAFGGELACVVLDATGNKA
SMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFALVIELMRSNKIHENLMKNQEFDFFSVGED
YQRNVVENKNMVKGVITF

Specific function: Putative L-galactonate oxidoreductase that is required for growth on L-galactonate as the sole carbon source [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=279, Percent_Identity=31.8996415770609, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI262073058, Length=208, Percent_Identity=30.2884615384615, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI71743840, Length=216, Percent_Identity=30.0925925925926, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4501939, Length=234, Percent_Identity=23.5042735042735, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI156523966, Length=234, Percent_Identity=23.5042735042735, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4501929, Length=244, Percent_Identity=28.6885245901639, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI226510992, Length=338, Percent_Identity=55.3254437869823, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1787863, Length=343, Percent_Identity=31.4868804664723, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788073, Length=342, Percent_Identity=30.4093567251462, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790045, Length=254, Percent_Identity=30.7086614173228, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082125, Length=276, Percent_Identity=31.1594202898551, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788407, Length=290, Percent_Identity=28.6206896551724, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790718, Length=307, Percent_Identity=27.3615635179153, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786825, Length=288, Percent_Identity=26.7361111111111, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788075, Length=339, Percent_Identity=26.2536873156342, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786552, Length=240, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081918, Length=306, Percent_Identity=26.1437908496732, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082401, Length=138, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=323, Percent_Identity=28.7925696594427, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17562878, Length=228, Percent_Identity=30.7017543859649, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71988145, Length=256, Percent_Identity=29.6875, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17562584, Length=321, Percent_Identity=25.5451713395639, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=339, Percent_Identity=26.2536873156342, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=347, Percent_Identity=28.5302593659942, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319955, Length=347, Percent_Identity=28.5302593659942, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323099, Length=345, Percent_Identity=28.1159420289855, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319257, Length=314, Percent_Identity=27.3885350318471, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319258, Length=230, Percent_Identity=26.9565217391304, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=286, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=290, Percent_Identity=26.2068965517241, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323980, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=288, Percent_Identity=26.0416666666667, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=266, Percent_Identity=24.4360902255639, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17137530, Length=225, Percent_Identity=34.6666666666667, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17737897, Length=186, Percent_Identity=38.1720430107527, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550770, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551930, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221457811, Length=187, Percent_Identity=28.8770053475936, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737895, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 36955; Mature: 36955

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLICQQPGVMEYVEKDIPTSADNEVLLKIKAVGICGTDIHAFAGRQPFFSYPRVLGHE
CCCEECCCCCHHHHHHHCCCCCCCCEEEEEEEEEEEECCCHHHHCCCCCCHHHHHHHHHH
ICAEAISRGSQCQTAQPGQRYSVIPCIPCGECAACREEKTNCCERVSLYGVHQDGGFSEY
HHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHE
LAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKAEQNVLVIGAGPIGLATAAIA
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
RAKGAHVVVADIDCQRRQHVVDHLAINAFDPTQEDFIVALREAFGGELACVVLDATGNKA
HCCCCEEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCC
SMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFALVIELMRSN
HHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
KIHENLMKNQEFDFFSVGEDYQRNVVENKNMVKGVITF
CHHHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MKTLICQQPGVMEYVEKDIPTSADNEVLLKIKAVGICGTDIHAFAGRQPFFSYPRVLGHE
CCCEECCCCCHHHHHHHCCCCCCCCEEEEEEEEEEEECCCHHHHCCCCCCHHHHHHHHHH
ICAEAISRGSQCQTAQPGQRYSVIPCIPCGECAACREEKTNCCERVSLYGVHQDGGFSEY
HHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHE
LAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKAEQNVLVIGAGPIGLATAAIA
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
RAKGAHVVVADIDCQRRQHVVDHLAINAFDPTQEDFIVALREAFGGELACVVLDATGNKA
HCCCCEEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCC
SMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKETTLLSSRNATREDFALVIELMRSN
HHHHHHHHHHCCCEEEEEEEEECEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
KIHENLMKNQEFDFFSVGEDYQRNVVENKNMVKGVITF
CHHHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]