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The map label for this gene is ydfI [H]

Identifier: 218550265

GI number: 218550265

Start: 3024639

End: 3026111

Strand: Direct

Name: ydfI [H]

Synonym: EFER_2957

Alternate gene names: 218550265

Gene position: 3024639-3026111 (Clockwise)

Preceding gene: 218550264

Following gene: 218550266

Centisome position: 65.91

GC content: 47.32

Gene sequence:

>1473_bases
ATGTCAGGGAACGAAAGTATCGCTGTCGCCCACTATGATCAGGTAACCACCTGGCCTCGGGACGAGTTACAGGCAGATAT
TGTCCATATTGGTTTTGGCGCTTTTCATCGCGGACACCAGGCTGTCTACATGGATCTCACTAATCAGCTTTCGGACACCC
GCTGGGGGATCTTTGAGATCAACCTTTTTGGTGATGCTCAACTGATTGAAAACTTAAATGCGCAAAATGGGCTGTTTTCG
GTTGTGGAAACATCTGCATCGCAATCTACCTCACGCCTTGTGCGTTCGGTGACTGGCGGTATTCACACCCCCGTGGATGG
CATTACCGCAGCAATTCAGAAATTGACTGAACCTCAGGTAACAATCGTTTCATTAACCATCACCGAGAAAGGCTATTGTC
TCGATTCGCAAACGCGATCACTTGATCTCACCAATGGATTAATCAAACACGATCTACAAAACCCGGATGCGCCTCAGTCG
GCTATTGGCTTGATCGTCTGTGCTTTGCAACAACGTAAAGCGGCAGGACTCGCCGCTTTTAGCGTGCTCTCCTGTGACAA
CTTGCCAGACAATGGGCATCTGACGCGAAATGCCGTTCTCGGTTTTGCCCGACAACTGGACCAGCCCCTGGCTCAGTGGA
TCGAAGAAAATGTCTCATTTCCAGGCACGATGGTAGATCGTATTGTTCCGGCAATGACCGAATCGCAATTCGCCTTACTG
GAAACTAAAACCGGTTATGCCGATCCCTGCGGGATTGTCTGCGAATCATTTCGCCAGTGGGTGATTGAAGATAATTTTGT
GCATGGGCGACCGGAATGGGATAAAGCAGGCGCGATGTTTGTCAGCAATGTTCAGCCTTACGAAGAAATGAAGTTACGCA
TGTTAAACGGTAGCCATTCATTTCTGGCCTATAACGGCTCACTGGCTGGCTATGAATTTATCTGGCAATGCATGGAAGAC
GCTGATTTTCGTTCCATTACCCGCCAGCTGATGATTAATGAACAAGCCCGAACACTTAATCCAGACTTAAATATCGACGC
CCATGAATACGCCGACCTGTTAATTGAACGCTTTAGCAACCGTAATGTTGCACACCGTACCGGGCAAATTGCTATGGACG
GTTCGCAAAAGCTTCCCCAGCGAGCGCTGACTCCCTGGCTGAAATTGCATCAGCAAAAACAAAACAATGCTGTTCTGTCA
CTGCTCGTGGCTGGTTGGTTGCATTATGTCATTGAAGCTGTTGAGAAAAGCCAGTCTGTCGCTGATCCAATGAATGAACA
ATTTCAGGCGCTAATAAAGGAACAACAAGACGCATGGCAACAGGCGCTCGCATTACTGCACCTTAGCGCCATATTTGGTG
ATTTAAGCAACCATCAGCCATTTATAAATGAAATAAAAATCGCATTTGCGAATATAAAAAACAAAGGCATCAAGGCCACC
ATCAGCCAATTATTATCGGATGAGCAGAAATGA

Upstream 100 bases:

>100_bases
CTTTTGCCTTTCTCTGCAAGCGTGATGCATCTCTCTGTTTTTGGTCTAGTGAATTATGTAAATTGGTCTATACACACCAG
CTACCAGGAGTAAGAGCATA

Downstream 100 bases:

>100_bases
AAACATTAATTTGTCAGCAGCCTGGCGTTATGGAATATGTGGAAAAGGATATTCCCACATCAGCAGATAATGAAGTGCTG
TTAAAAATCAAAGCTGTGGG

Product: Fructuronate reductase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSGNESIAVAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYMDLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFS
VVETSASQSTSRLVRSVTGGIHTPVDGITAAIQKLTEPQVTIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
ETKTGYADPCGIVCESFRQWVIEDNFVHGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMED
ADFRSITRQLMINEQARTLNPDLNIDAHEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLS
LLVAGWLHYVIEAVEKSQSVADPMNEQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
ISQLLSDEQK

Sequences:

>Translated_490_residues
MSGNESIAVAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYMDLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFS
VVETSASQSTSRLVRSVTGGIHTPVDGITAAIQKLTEPQVTIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
ETKTGYADPCGIVCESFRQWVIEDNFVHGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMED
ADFRSITRQLMINEQARTLNPDLNIDAHEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLS
LLVAGWLHYVIEAVEKSQSVADPMNEQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
ISQLLSDEQK
>Mature_489_residues
SGNESIAVAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYMDLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSV
VETSASQSTSRLVRSVTGGIHTPVDGITAAIQKLTEPQVTIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSA
IGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALLE
TKTGYADPCGIVCESFRQWVIEDNFVHGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDA
DFRSITRQLMINEQARTLNPDLNIDAHEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSL
LVAGWLHYVIEAVEKSQSVADPMNEQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKATI
SQLLSDEQK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=470, Percent_Identity=49.5744680851064, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1790779, Length=474, Percent_Identity=49.1561181434599, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1788497, Length=480, Percent_Identity=47.9166666666667, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI48994885, Length=398, Percent_Identity=25.3768844221106, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790028, Length=282, Percent_Identity=23.4042553191489, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324401, Length=479, Percent_Identity=34.0292275574113, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6320765, Length=479, Percent_Identity=34.0292275574113, Blast_Score=286, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54599; Mature: 54468

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNESIAVAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYMDLTNQLSDTRWGIFEI
CCCCCCEEEEECCCCCCCCCHHHHHHHHEECHHHHHCCCEEEEEEHHHHCCCCCCCEEEE
NLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIHTPVDGITAAIQKLTEPQV
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCE
TIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSAIGLIVCALQQRKAAGLAAF
EEEEEEEECCCCEECCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
ETKTGYADPCGIVCESFRQWVIEDNFVHGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHS
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCC
FLAYNGSLAGYEFIWQCMEDADFRSITRQLMINEQARTLNPDLNIDAHEYADLLIERFSN
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCC
RNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLHYVIEAVEKSQSV
CCCHHCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPMNEQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
ISQLLSDEQK
HHHHHCCCCC
>Mature Secondary Structure 
SGNESIAVAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYMDLTNQLSDTRWGIFEI
CCCCCEEEEECCCCCCCCCHHHHHHHHEECHHHHHCCCEEEEEEHHHHCCCCCCCEEEE
NLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIHTPVDGITAAIQKLTEPQV
EECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCE
TIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQSAIGLIVCALQQRKAAGLAAF
EEEEEEEECCCCEECCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRIVPAMTESQFALL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEE
ETKTGYADPCGIVCESFRQWVIEDNFVHGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHS
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCC
FLAYNGSLAGYEFIWQCMEDADFRSITRQLMINEQARTLNPDLNIDAHEYADLLIERFSN
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCC
RNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLHYVIEAVEKSQSV
CCCHHCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPMNEQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIAFANIKNKGIKAT
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHH
ISQLLSDEQK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]