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The map label for this gene is fumB [H]

Identifier: 218549705

GI number: 218549705

Start: 2440787

End: 2442439

Strand: Direct

Name: fumB [H]

Synonym: EFER_2382

Alternate gene names: 218549705

Gene position: 2440787-2442439 (Clockwise)

Preceding gene: 218549704

Following gene: 218549715

Centisome position: 53.19

GC content: 46.04

Gene sequence:

>1653_bases
ATGTCTAAGCCCTTTGTCTGGCAGGAGCTCTTTACGTTAAGTGAAGATATTACCGAGTATGAATTATTCAGTGATCAATA
TATTACTGTTACACAGCTTGACGATAACGAAATCATTAAAATCGCCCCGGAAGCCTTAACATTACTGGCACAACGGGCAT
TTCATGATGCATCTTTTTTCTTGCGTACTGAACATTTAGCACAGGTCGCCAGTATTCTGCTCGATCCCCATGCCAGCAGT
AACGACAAGTATGTGGCTTTGCAACTGCTGCGCAACGCTGAAGTGTCAGCCGGAGGGATTCTGCCTAACTGTCAGGACAC
AGGAACAGCGACGATTATCGCCAGCAAAGGGCAAAATATTTGGACAGGTGTTAACGATGCCGAATACTTAAGTAAAGGAA
TTTACACCACCTTTCAGGAAAATAATCTGCGCTATTCGCAGAATGCACCGCTGGATATGTATAACGAAATTAATACGCAA
ACTAACCTACCAGCACAAATAGATATCAGCGCCACACCAGGTAGTGAATACCAATTTTTATTTATAAATAAAGGGGGTGG
TTCAGCCAATAAAGCAGCCTTGTTTCAGGAAACGAAATCTATTTTACAACCAGAAAAACTGACCCAGTTTCTGATTGATA
AAATGAAGTCTCTGGGTACTGCGGCCTGTCCACCATACCATATCGCTTTTGTCGTGGGCGGGCTTTCAGCAGACCAGGCG
TTAAAGGTAGCCAAACTGGCATCAACTAAATATTACGATTATCTCCCCACCATCGGTAATGAACTGGGCCAGGCATTTCG
TGATATGGCATTGGAAGTTACTCTCCTTAATGCCAGCCGGGAATTTGGTATAGGGGCACAATTTGGCGGCAAATATTTTG
CTCATGATATTCGGGTGATTCGTCTGCCGCGTCATGGCGGCTCTTGCCCGATTGCGATGGCCCTCTCCTGCTCCGCTGAT
CGCAATATCAAAGCCAAAATTAACAAACAGGGTATCTGGCTGGAAAAACTCGAACGTAATCCCGGACGTTTGATACCGCA
ACCGCTACGTACGGAAAGCAATTCTCAGGTAGTGGAAATTGATCTGAATCGACCGTTAGATGCAATCAGAAGTGATTTAT
CTCGTTTGCCCATTGGCGCACGCCTCTCACTAACTGGCCCTATAGTTGTCGCCCGGGATATTGCCCATGCAAAAATTAAA
ACCCGATTAGAGTATGGCGAACCGCTACCAGAATATATGAAAAAACACATTGTATATTACGCCGGACCAGCAAAAACACC
GGAAGGTTACCCTTGCGGTTCTCTGGGCCCGACGACCGGTGGTCGGATGGATGGCTACGTAGATGCATTTCAGGCCGCAG
GTGGTAGCCTGGTGATGCTCTCAAAAGGCAATCGTAGTCAACAGGTTACCGATGCCTGCAAAAAACATGGTGGTTTTAAT
CTCGGCAGTATTGGTGGTGCGGCGGCACTTCTGGCCCAGCAATATGTGAAAAGCCTGCGTTGTATTGAATATCCTGAACT
GGGTATGGAAGCAGTGTGGATGATGGAAGTGGAACACCTGCCTGCTTTTCTGTTGGTGGATGACAAAGGAAATAACTTCT
TTAGTCAGTTTGAGCAGTCATGCCATTGCAACAGTTGTCTGGCAGGCAAATAA

Upstream 100 bases:

>100_bases
AAAGCGAGACTGAACTGTCAGAAATGGAGCTACCTTAATTCGTTTCGCCTGGCGTCACAATTAACCTAACTTTGCCACCT
ACTTTTAAGATGAGAAAAAA

Downstream 100 bases:

>100_bases
AAGATGCTCAAAGCATGGTGGTTTGCGACTTTTCCCGCAGCCAGTCACGAAAAATCTGCAAATGGCGGGATTTATTTTTC
TCTTCCCGCCACGTCAAAAC

Product: Fumarate hydratase class I subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSKPFVWQELFTLSEDITEYELFSDQYITVTQLDDNEIIKIAPEALTLLAQRAFHDASFFLRTEHLAQVASILLDPHASS
NDKYVALQLLRNAEVSAGGILPNCQDTGTATIIASKGQNIWTGVNDAEYLSKGIYTTFQENNLRYSQNAPLDMYNEINTQ
TNLPAQIDISATPGSEYQFLFINKGGGSANKAALFQETKSILQPEKLTQFLIDKMKSLGTAACPPYHIAFVVGGLSADQA
LKVAKLASTKYYDYLPTIGNELGQAFRDMALEVTLLNASREFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSAD
RNIKAKINKQGIWLEKLERNPGRLIPQPLRTESNSQVVEIDLNRPLDAIRSDLSRLPIGARLSLTGPIVVARDIAHAKIK
TRLEYGEPLPEYMKKHIVYYAGPAKTPEGYPCGSLGPTTGGRMDGYVDAFQAAGGSLVMLSKGNRSQQVTDACKKHGGFN
LGSIGGAAALLAQQYVKSLRCIEYPELGMEAVWMMEVEHLPAFLLVDDKGNNFFSQFEQSCHCNSCLAGK

Sequences:

>Translated_550_residues
MSKPFVWQELFTLSEDITEYELFSDQYITVTQLDDNEIIKIAPEALTLLAQRAFHDASFFLRTEHLAQVASILLDPHASS
NDKYVALQLLRNAEVSAGGILPNCQDTGTATIIASKGQNIWTGVNDAEYLSKGIYTTFQENNLRYSQNAPLDMYNEINTQ
TNLPAQIDISATPGSEYQFLFINKGGGSANKAALFQETKSILQPEKLTQFLIDKMKSLGTAACPPYHIAFVVGGLSADQA
LKVAKLASTKYYDYLPTIGNELGQAFRDMALEVTLLNASREFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSAD
RNIKAKINKQGIWLEKLERNPGRLIPQPLRTESNSQVVEIDLNRPLDAIRSDLSRLPIGARLSLTGPIVVARDIAHAKIK
TRLEYGEPLPEYMKKHIVYYAGPAKTPEGYPCGSLGPTTGGRMDGYVDAFQAAGGSLVMLSKGNRSQQVTDACKKHGGFN
LGSIGGAAALLAQQYVKSLRCIEYPELGMEAVWMMEVEHLPAFLLVDDKGNNFFSQFEQSCHCNSCLAGK
>Mature_549_residues
SKPFVWQELFTLSEDITEYELFSDQYITVTQLDDNEIIKIAPEALTLLAQRAFHDASFFLRTEHLAQVASILLDPHASSN
DKYVALQLLRNAEVSAGGILPNCQDTGTATIIASKGQNIWTGVNDAEYLSKGIYTTFQENNLRYSQNAPLDMYNEINTQT
NLPAQIDISATPGSEYQFLFINKGGGSANKAALFQETKSILQPEKLTQFLIDKMKSLGTAACPPYHIAFVVGGLSADQAL
KVAKLASTKYYDYLPTIGNELGQAFRDMALEVTLLNASREFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADR
NIKAKINKQGIWLEKLERNPGRLIPQPLRTESNSQVVEIDLNRPLDAIRSDLSRLPIGARLSLTGPIVVARDIAHAKIKT
RLEYGEPLPEYMKKHIVYYAGPAKTPEGYPCGSLGPTTGGRMDGYVDAFQAAGGSLVMLSKGNRSQQVTDACKKHGGFNL
GSIGGAAALLAQQYVKSLRCIEYPELGMEAVWMMEVEHLPAFLLVDDKGNNFFSQFEQSCHCNSCLAGK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=546, Percent_Identity=65.2014652014652, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=545, Percent_Identity=64.7706422018349, Blast_Score=762, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=28.4916201117318, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60337; Mature: 60205

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPFVWQELFTLSEDITEYELFSDQYITVTQLDDNEIIKIAPEALTLLAQRAFHDASFF
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHCCHHHH
LRTEHLAQVASILLDPHASSNDKYVALQLLRNAEVSAGGILPNCQDTGTATIIASKGQNI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEEEEECCCEE
WTGVNDAEYLSKGIYTTFQENNLRYSQNAPLDMYNEINTQTNLPAQIDISATPGSEYQFL
ECCCCHHHHHHCCCEEEEECCCEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEE
FINKGGGSANKAALFQETKSILQPEKLTQFLIDKMKSLGTAACPPYHIAFVVGGLSADQA
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHH
LKVAKLASTKYYDYLPTIGNELGQAFRDMALEVTLLNASREFGIGAQFGGKYFAHDIRVI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEECEEEE
RLPRHGGSCPIAMALSCSADRNIKAKINKQGIWLEKLERNPGRLIPQPLRTESNSQVVEI
EECCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEE
DLNRPLDAIRSDLSRLPIGARLSLTGPIVVARDIAHAKIKTRLEYGEPLPEYMKKHIVYY
ECCCCHHHHHHHHHHCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHCEEEE
AGPAKTPEGYPCGSLGPTTGGRMDGYVDAFQAAGGSLVMLSKGNRSQQVTDACKKHGGFN
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
LGSIGGAAALLAQQYVKSLRCIEYPELGMEAVWMMEVEHLPAFLLVDDKGNNFFSQFEQS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHC
CHCNSCLAGK
CCCCCCCCCC
>Mature Secondary Structure 
SKPFVWQELFTLSEDITEYELFSDQYITVTQLDDNEIIKIAPEALTLLAQRAFHDASFF
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHCCHHHH
LRTEHLAQVASILLDPHASSNDKYVALQLLRNAEVSAGGILPNCQDTGTATIIASKGQNI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEEEEECCCEE
WTGVNDAEYLSKGIYTTFQENNLRYSQNAPLDMYNEINTQTNLPAQIDISATPGSEYQFL
ECCCCHHHHHHCCCEEEEECCCEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEE
FINKGGGSANKAALFQETKSILQPEKLTQFLIDKMKSLGTAACPPYHIAFVVGGLSADQA
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHH
LKVAKLASTKYYDYLPTIGNELGQAFRDMALEVTLLNASREFGIGAQFGGKYFAHDIRVI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEEEECEEEE
RLPRHGGSCPIAMALSCSADRNIKAKINKQGIWLEKLERNPGRLIPQPLRTESNSQVVEI
EECCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEE
DLNRPLDAIRSDLSRLPIGARLSLTGPIVVARDIAHAKIKTRLEYGEPLPEYMKKHIVYY
ECCCCHHHHHHHHHHCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHCEEEE
AGPAKTPEGYPCGSLGPTTGGRMDGYVDAFQAAGGSLVMLSKGNRSQQVTDACKKHGGFN
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
LGSIGGAAALLAQQYVKSLRCIEYPELGMEAVWMMEVEHLPAFLLVDDKGNNFFSQFEQS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHC
CHCNSCLAGK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]