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The map label for this gene is setB [H]

Identifier: 218549584

GI number: 218549584

Start: 2316288

End: 2317547

Strand: Direct

Name: setB [H]

Synonym: EFER_2257

Alternate gene names: 218549584

Gene position: 2316288-2317547 (Clockwise)

Preceding gene: 218549577

Following gene: 218549586

Centisome position: 50.48

GC content: 48.41

Gene sequence:

>1260_bases
GTGCTTGATTTGGCAACCTGTTTATTTTCTGACAAGCCGCGTAAACTCCGCATCTCTTTACGTCACAGATACCGATGTAT
GCATAACTCCCCTGCTGTCTCCACGCCTAAGGCGTTTGATTTGACTGCCACGGCATTTCTCATCGTGGCCTTTCTCACCG
GTATTGCCGGGGCGCTACAAACTCCCACATTAAGTTTGTTTCTGACGGATGAAGTCCATGCCCGGCCTGGTATGGTTGGT
TTCTTTTTTACCGGCAGTGCGGTGATCGGCATTCTGGTAAGTCAATTTCTGGCAGGACGTTCAGATAAAAAAGGTGATCG
TAAAAAGTTGATTGTCTTTTGTTGTCTGATGGGCACGTTGGCTTGTGTACTTTTCGCCTGGAATCGCAATTACTTTATTT
TGTTGTTTCTTGGTGTATTTCTTAGCAGTTTCGGTTCGACTTCCAACCCACAAATGTTTGCTCTGGCGCGGGAACATGCC
GATCGCACGGGTCGGGAAGCGGTGATGTTTAGTTCATTTTTGCGCGCTCAGGTGTCTCTGGCCTGGGTGATTGGTCCGCC
GCTGGCTTATGCGTTAGCCATGGGGTTTGGTTTCACCGTGATGTACCTCAGCGCTGCGGTAGCATTTATTGTCTGTGGCG
TGATGGTATGGCTGTTTTTACCTTCAATGCGCAAAGATGCTCCTCTCGCCGTTGGAACACTGGAAGCGCCACGTCGTAAT
CGTCGCGATACCCTGTTGCTATTTGCTATCTGTACGCTGATGTGGGGCACTAACAGCCTCTATATCATTAATATGCCGCT
GTTTATCATTAATGAATTGCATCTACCGGAAAAACTTGCAGGTGTGATGATGGGTACAGCAGCAGGCCTGGAAATACCCA
CAATGCTGATTGCAGGTTATTTTGCTAAACGGTTGGGTAAACGCCTGCTGATGATCATTGCCGTAGCAGCAGGTTTTGCA
TTTTATGCAGGAATGTTGCTCGCTCACTCACCAATGGTATTGCTCGGCTTGCAGCTGCTGAACGCTATTTATATTGGGAT
TCTCGGCGGTATTGGAATGCTCTATTTTCAGGATTTAATGCCCGGACAGGCAGGATCAGCCACCACGCTCTATACCAATA
CGATTCGTGTTGGCTGGATTATCGCCGGATCGCTGGCCGGAATCGCCGCTGAACTGTGGAGTTATCATTCTGTCTTCTGG
TTCGCATTAGCAATGACCGTTGCTGCCGTGTTTTGTCTTTTCAAAATTCGGGACGTTTAG

Upstream 100 bases:

>100_bases
CACAATGCACATTTTTACGTCTAAAAATCTTATTTGCTGAACGATACATTACTTCAGCCAGCACGTTTTCAGCTGATTTC
AGCCTTTAACATTTCGTGGC

Downstream 100 bases:

>100_bases
GGAGCTGTCAGGGCTTCAAGTTCAATAAGCCAGGTCATCGCTTGCTGACGCGTTTGCCCGCACATTTCAGCACTGGGTTG
TAACCCGGCACAGACTTTAG

Product: lactose/glucose efflux system

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLDLATCLFSDKPRKLRISLRHRYRCMHNSPAVSTPKAFDLTATAFLIVAFLTGIAGALQTPTLSLFLTDEVHARPGMVG
FFFTGSAVIGILVSQFLAGRSDKKGDRKKLIVFCCLMGTLACVLFAWNRNYFILLFLGVFLSSFGSTSNPQMFALAREHA
DRTGREAVMFSSFLRAQVSLAWVIGPPLAYALAMGFGFTVMYLSAAVAFIVCGVMVWLFLPSMRKDAPLAVGTLEAPRRN
RRDTLLLFAICTLMWGTNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMIIAVAAGFA
FYAGMLLAHSPMVLLGLQLLNAIYIGILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAELWSYHSVFW
FALAMTVAAVFCLFKIRDV

Sequences:

>Translated_419_residues
MLDLATCLFSDKPRKLRISLRHRYRCMHNSPAVSTPKAFDLTATAFLIVAFLTGIAGALQTPTLSLFLTDEVHARPGMVG
FFFTGSAVIGILVSQFLAGRSDKKGDRKKLIVFCCLMGTLACVLFAWNRNYFILLFLGVFLSSFGSTSNPQMFALAREHA
DRTGREAVMFSSFLRAQVSLAWVIGPPLAYALAMGFGFTVMYLSAAVAFIVCGVMVWLFLPSMRKDAPLAVGTLEAPRRN
RRDTLLLFAICTLMWGTNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMIIAVAAGFA
FYAGMLLAHSPMVLLGLQLLNAIYIGILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAELWSYHSVFW
FALAMTVAAVFCLFKIRDV
>Mature_419_residues
MLDLATCLFSDKPRKLRISLRHRYRCMHNSPAVSTPKAFDLTATAFLIVAFLTGIAGALQTPTLSLFLTDEVHARPGMVG
FFFTGSAVIGILVSQFLAGRSDKKGDRKKLIVFCCLMGTLACVLFAWNRNYFILLFLGVFLSSFGSTSNPQMFALAREHA
DRTGREAVMFSSFLRAQVSLAWVIGPPLAYALAMGFGFTVMYLSAAVAFIVCGVMVWLFLPSMRKDAPLAVGTLEAPRRN
RRDTLLLFAICTLMWGTNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRLLMIIAVAAGFA
FYAGMLLAHSPMVLLGLQLLNAIYIGILGGIGMLYFQDLMPGQAGSATTLYTNTIRVGWIIAGSLAGIAAELWSYHSVFW
FALAMTVAAVFCLFKIRDV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport lactose and glucose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI1788495, Length=393, Percent_Identity=87.5318066157761, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1790091, Length=393, Percent_Identity=68.9567430025445, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI48994875, Length=384, Percent_Identity=53.3854166666667, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45818; Mature: 45818

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLATCLFSDKPRKLRISLRHRYRCMHNSPAVSTPKAFDLTATAFLIVAFLTGIAGALQ
CCCHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
TPTLSLFLTDEVHARPGMVGFFFTGSAVIGILVSQFLAGRSDKKGDRKKLIVFCCLMGTL
CCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ACVLFAWNRNYFILLFLGVFLSSFGSTSNPQMFALAREHADRTGREAVMFSSFLRAQVSL
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
AWVIGPPLAYALAMGFGFTVMYLSAAVAFIVCGVMVWLFLPSMRKDAPLAVGTLEAPRRN
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
RRDTLLLFAICTLMWGTNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGY
CCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
FAKRLGKRLLMIIAVAAGFAFYAGMLLAHSPMVLLGLQLLNAIYIGILGGIGMLYFQDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGQAGSATTLYTNTIRVGWIIAGSLAGIAAELWSYHSVFWFALAMTVAAVFCLFKIRDV
CCCCCCCEEEEECCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDLATCLFSDKPRKLRISLRHRYRCMHNSPAVSTPKAFDLTATAFLIVAFLTGIAGALQ
CCCHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
TPTLSLFLTDEVHARPGMVGFFFTGSAVIGILVSQFLAGRSDKKGDRKKLIVFCCLMGTL
CCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ACVLFAWNRNYFILLFLGVFLSSFGSTSNPQMFALAREHADRTGREAVMFSSFLRAQVSL
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
AWVIGPPLAYALAMGFGFTVMYLSAAVAFIVCGVMVWLFLPSMRKDAPLAVGTLEAPRRN
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
RRDTLLLFAICTLMWGTNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGY
CCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
FAKRLGKRLLMIIAVAAGFAFYAGMLLAHSPMVLLGLQLLNAIYIGILGGIGMLYFQDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGQAGSATTLYTNTIRVGWIIAGSLAGIAAELWSYHSVFWFALAMTVAAVFCLFKIRDV
CCCCCCCEEEEECCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10209755; 10438463 [H]