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The map label for this gene is yeiL [H]

Identifier: 218549577

GI number: 218549577

Start: 2307890

End: 2308549

Strand: Direct

Name: yeiL [H]

Synonym: EFER_2249

Alternate gene names: 218549577

Gene position: 2307890-2308549 (Clockwise)

Preceding gene: 218549573

Following gene: 218549584

Centisome position: 50.29

GC content: 42.88

Gene sequence:

>660_bases
ATGAGTGAGTCCGCGTATAAGGATTGCTTTTCAACGGATGTTTCAGCCGATGCACGGCTGTTTCATTTTTTAGCACGTGA
CTACATTGTGCAGGAAGGGCAACAGCCGTTATGGCTGTTTTACCTGACGCGAGGCCGCGCCAGGCTTTACGCCACGCTTG
CTAATGGTCGTGTGTCGCTGATTGATTTCTTTGCTGCACCCTGTTTTATTGGTGAGATTGAGTTAATCGATAAAGATCAT
GAACCACGAGCGGTACAGGCGATTGAAGAGTGTTGGTGCCTTGCACTCCCCATGAAACATTACCGTCCGCTGTTATTAAA
CGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATC
AATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAACATACC
CAGGCAGCAGAGTATTTAGGTGTTACTTATCGTCACTTACTATATGTGATCGCTCAACTCATTCACGACGGTTTATTAAT
TAAAAGTAAGAAAGGGTATTTAATTAAAAACAGAAAGCAGTTGTTAGGGCTGGCACTGGAGATGGACCCGGAGAATAAAT
TCTCCGGGATGATGCAGTAA

Upstream 100 bases:

>100_bases
TTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATA
ATGATCTTAAGCAGCAATTG

Downstream 100 bases:

>100_bases
AAAATAATCTGGAATAGCGCGATTGCCGGATGCAACGCTTAATAAGCGCGTACATCCGGTAATGAAACTCAAGCTAAACC
AATAAAGAACCCGGCAATGG

Product: DNA-binding transcriptional activator YeiL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSESAYKDCFSTDVSADARLFHFLARDYIVQEGQQPLWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHT
QAAEYLGVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLLGLALEMDPENKFSGMMQ

Sequences:

>Translated_219_residues
MSESAYKDCFSTDVSADARLFHFLARDYIVQEGQQPLWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHT
QAAEYLGVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLLGLALEMDPENKFSGMMQ
>Mature_218_residues
SESAYKDCFSTDVSADARLFHFLARDYIVQEGQQPLWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHE
PRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQ
AAEYLGVTYRHLLYVIAQLIHDGLLIKSKKGYLIKNRKQLLGLALEMDPENKFSGMMQ

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=219, Percent_Identity=96.3470319634703, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 25287; Mature: 25156

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESAYKDCFSTDVSADARLFHFLARDYIVQEGQQPLWLFYLTRGRARLYATLANGRVSL
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLS
HHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVTYRHLLYVIAQL
CCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLLGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEECCHHHHHHHHEEECCCCCCCCCCC
>Mature Secondary Structure 
SESAYKDCFSTDVSADARLFHFLARDYIVQEGQQPLWLFYLTRGRARLYATLANGRVSL
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLS
HHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVTYRHLLYVIAQL
CCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLLGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEECCHHHHHHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]