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The map label for this gene is fruA [H]

Identifier: 218549581

GI number: 218549581

Start: 2312253

End: 2313980

Strand: Reverse

Name: fruA [H]

Synonym: EFER_2254

Alternate gene names: 218549581

Gene position: 2313980-2312253 (Counterclockwise)

Preceding gene: 218549582

Following gene: 218549580

Centisome position: 50.43

GC content: 52.84

Gene sequence:

>1728_bases
GTGTCGACTTACAACCGTTTAACTGACAGCAGGAGAGGCATAATGAAAACGCTGCTGATTATTGACGCTAATCTCGGTCA
GGCTCGCGCCTATATGGCGAAAACCCTGCTGGGCGCGGCGGCGCATAAAGCGAATCTGGATATTATCGACAACCCAAATG
ATGCCGATATGGCTATTATTCTGGGTAGCACTATGCCTGCCGACAGTGCCCTGAACGGTAAAAAGGTCTGGCTGGGTGAT
ATCAACCGTGCGGTTGCACATCCTGAACTGTTTCTGAGTGAAGCAAAAGGTCATGCCAAACCGTACAGTTCTCCGGCTGT
TGCTACGCCTGTTCCTGCCAGCGGACAGAAACGTGTAGTTGCGGTGACAGCTTGTCCGACAGGTGTTGCTCATACCTTTA
TGGCGGCAGAAGCCATTGAAACCGAAGCGAAAAAACGCGGCTGGTGGGTGAAAGTTGAAACCCGTGGTTCTGTGGGAGCG
GGTAATGCCATTACTCCGGAAGAGGTGGCTGCGGCAGATCTGGTGATTGTAGCGGCAGATATCGAAGTGGATCTGGCCAA
ATTTGCCGGTAAACCGATGTACCGTACTTCAACCGGACTGGCGCTGAAGAAAACCGCTCAGGAACTGGATAAAGCAGTAG
CAGAAGCCAAACCATATGAACCTGCGGGGCAATCTGCCACTCCAACGGAAAGTAAAAAAGAGAGTGCTGGCGCCTATCGT
CACCTGTTGACGGGTGTTTCTTATATGCTGCCGATGGTGGTTGCTGGTGGTCTGTGTATCGCCCTTTCTTTTGCCTTCGG
TATCGAAGCGTTCAAAGAACCGGGTACGCTGGCAGCGGCTTTGATGCAAATTGGTGGTGGTTCAGCCTTTGCGCTAATGG
TACCGGTACTGGCGGGATATATTGCGTTTTCTATTGCGGACCGCCCAGGTCTGACGCCAGGTTTGATTGGCGGTATGTTG
GCTGTCAGCACCGGCTCAGGTTTTATCGGCGGCATCATTGCTGGTTTCCTGGCTGGATATATGGCGAAGCTCATTAGCAC
TCAGCTTAAGCTGCCACAAAGTATGGAAGCGCTGAAACCGATTCTGATTATTCCGCTAATCTCCAGCCTGGTGGTTGGTC
TGGCGATGATCTACCTTATCGGTAAACCTGTCGCAGGTATTCTGGAAGGCTTGACGCACTGGCTGCAAACCATGGGTACT
GCGAATGCTGTTCTGCTGGGAGCGATCCTCGGCGGCATGATGTGTACCGATATGGGTGGTCCGGTAAACAAAGCAGCATA
TGCGTTTGGTGTTGGTCTGCTGAGTACGCAAACTTACGCACCGATGGCGGCGATCATGGCTGCGGGTATGGTTCCACCGC
TGGCGATGGGTCTGGCAACAATGGTGGCACGGCGCAAATTCGACAAAGCGCAGCAGGAAGGTGGTAAAGCAGCTCTGGTA
CTGGGACTGTGCTTTATTTCTGAAGGTGCGATTCCGTTTGCTGCCCGTGATCCGATGCGTGTACTTCCGTGCTGTATCGT
TGGTGGCGCGCTGACAGGCGCAATTTCGATGGCGATTGGCGCGAAACTGATGGCACCACACGGTGGTCTGTTTGTGCTGC
TCATCCCTGGTGCAATCACTCCAGTAGTGGGTTATCTGGTGGCAATTATCGCGGGTACGTTGGTGGCTGGTTTGTCTTAC
GCCTTCCTCAAACGTCCCGAAACACAAGCAGTAGAAAAAACCGCATAA

Upstream 100 bases:

>100_bases
GAACACACGTTACGCCTGGCAACCGCCGTAGCTGCCCTGGCGGTTAGTCAAAGTAACGTCGGTATTACTGATCGTCCTCA
GTTGGCCGCAATGATGGCGC

Downstream 100 bases:

>100_bases
TTCCTCTTCATCATCAGGGCAGAGTCTTCTCTGCCCTTTTCCTTTCCCTGTTCTTATATTCTCTAAATAATTCGAGTTGC
AGGACACAACGGCAGTGGCG

Product: PTS system fructose-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSTYNRLTDSRRGIMKTLLIIDANLGQARAYMAKTLLGAAAHKANLDIIDNPNDADMAIILGSTMPADSALNGKKVWLGD
INRAVAHPELFLSEAKGHAKPYSSPAVATPVPASGQKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGA
GNAITPEEVAAADLVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEAKPYEPAGQSATPTESKKESAGAYR
HLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGML
AVSTGSGFIGGIIAGFLAGYMAKLISTQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGT
ANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALV
LGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAITPVVGYLVAIIAGTLVAGLSY
AFLKRPETQAVEKTA

Sequences:

>Translated_575_residues
MSTYNRLTDSRRGIMKTLLIIDANLGQARAYMAKTLLGAAAHKANLDIIDNPNDADMAIILGSTMPADSALNGKKVWLGD
INRAVAHPELFLSEAKGHAKPYSSPAVATPVPASGQKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGA
GNAITPEEVAAADLVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEAKPYEPAGQSATPTESKKESAGAYR
HLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGML
AVSTGSGFIGGIIAGFLAGYMAKLISTQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGT
ANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALV
LGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAITPVVGYLVAIIAGTLVAGLSY
AFLKRPETQAVEKTA
>Mature_574_residues
STYNRLTDSRRGIMKTLLIIDANLGQARAYMAKTLLGAAAHKANLDIIDNPNDADMAIILGSTMPADSALNGKKVWLGDI
NRAVAHPELFLSEAKGHAKPYSSPAVATPVPASGQKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAG
NAITPEEVAAADLVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEAKPYEPAGQSATPTESKKESAGAYRH
LLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLA
VSTGSGFIGGIIAGFLAGYMAKLISTQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTA
NAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALVL
GLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAITPVVGYLVAIIAGTLVAGLSYA
FLKRPETQAVEKTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=563, Percent_Identity=93.9609236234458, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1786951, Length=552, Percent_Identity=33.5144927536232, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI87082348, Length=451, Percent_Identity=33.0376940133038, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1790386, Length=301, Percent_Identity=42.1926910299003, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1788729, Length=384, Percent_Identity=27.6041666666667, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=53.4090909090909, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788730, Length=103, Percent_Identity=37.8640776699029, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=44.6153846153846, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 59163; Mature: 59031

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYNRLTDSRRGIMKTLLIIDANLGQARAYMAKTLLGAAAHKANLDIIDNPNDADMAII
CCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEE
LGSTMPADSALNGKKVWLGDINRAVAHPELFLSEAKGHAKPYSSPAVATPVPASGQKRVV
EECCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
AVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAAD
EEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCEEEEEEC
IEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEAKPYEPAGQSATPTESKKESAGAYR
CCHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
HLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
IAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYMAKLISTQLKLPQSMEALKP
HHHHCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGMMCTDMGG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
PVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
LGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAIT
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCHHH
PVVGYLVAIIAGTLVAGLSYAFLKRPETQAVEKTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
STYNRLTDSRRGIMKTLLIIDANLGQARAYMAKTLLGAAAHKANLDIIDNPNDADMAII
CCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEE
LGSTMPADSALNGKKVWLGDINRAVAHPELFLSEAKGHAKPYSSPAVATPVPASGQKRVV
EECCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
AVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAAD
EEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCEEEEEEC
IEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEAKPYEPAGQSATPTESKKESAGAYR
CCHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
HLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
IAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYMAKLISTQLKLPQSMEALKP
HHHHCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAVLLGAILGGMMCTDMGG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
PVNKAAYAFGVGLLSTQTYAPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
LGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAIT
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCHHH
PVVGYLVAIIAGTLVAGLSYAFLKRPETQAVEKTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]