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The map label for this gene is dusC [H]

Identifier: 218549553

GI number: 218549553

Start: 2282102

End: 2283052

Strand: Reverse

Name: dusC [H]

Synonym: EFER_2225

Alternate gene names: 218549553

Gene position: 2283052-2282102 (Counterclockwise)

Preceding gene: 218549561

Following gene: 218549552

Centisome position: 49.75

GC content: 50.05

Gene sequence:

>951_bases
GTGGGTAAAAATATGCGTGTGTTACTGGCACCGATGGAAGGCGTGCTCGACTCTCTGGTGCGTGAATTACTGACCGAAGT
AAACGATTACGATCTTTGTATCACCGAATTTGTTCGCGTGGTTGATCAACTCCTGCCAGTTAAAGTCTTTCAACGTATCT
GCCCGGAGCTGCAAAATAGTAGTCGGACACCTTCCGGTACGCTGGTGCGCATCCAATTACTTGGTCAATATCCACAGTGG
TTGGCGGAGAATGCTCTCAGGGCGGTGGAACTGGGATCATTTGGGGTTGATCTTAACTGCGGCTGTCCTTCAAAAACAGT
TAATGGTAGCGGTGGCGGAGCGACATTATTAAAAGATCCTGAACTTATCTACCAGGGCGCGAAAGCGATGCGCGAAGCGG
TGCCTGCTCATTTGCCAGTGAGTGTAAAAGTGCGTTTGGGATGGGACAGTGGTGAGCAAAAATTTGAAATTGCCGATGCT
GTGCAGCAAGCGGGGGCCACGGAGCTTGTGGTTCACGGGCGGACGAAAGAGCAGGGCTATCGCGCCGAGCATATTGACTG
GCAGGCGATTGGTGAAATCCGTCAGCGACTGACGATTCCGGTGATCGCGAATGGAGAAATCTGGGACTGGCAGAGCGCAC
AGCAATGTATGGCGTTAAGTGGCTGTGATGCCGTTATGATTGGGCGCGGGGCATTAAATATTCCCAACTTAAGTCGGGTG
GTGAAATACAACGAGCCGCGTATGCCGTGGGCACAAGTTGTTGCATTATTGCAGAAATATACGCGTCTGGAAAAACAGGG
CGATACCGGGCTATATCACGTGGCACGAATTAAACAGTGGTTAGGTTATCTGCGTAAAGAATACAGCGAAGCGACAACAT
TATTTCAGCAAATCCGCGCATTAAATAATTCCCCTGCTATCGCGCAGGCTATTCAGGCGATAAAACTGTAA

Upstream 100 bases:

>100_bases
ACATAGACAAAGCATAGAGTCGTCTCCAGGCTTTGGTGCACAGATATTGAAGCACTTTAAACAGTAGAGCGGAGACGTTA
CCATAGCGCCTCATTTTTTC

Downstream 100 bases:

>100_bases
TCGTTGCAAGGCCGGGATAACTACCCGGCCTTGTGGGAAATCTTCAGAAAATCATTTTAAAAACGCCCGTCACTACCAGT
AACCCGATAATAAATATAAC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MGKNMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFQRICPELQNSSRTPSGTLVRIQLLGQYPQW
LAENALRAVELGSFGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEQKFEIADA
VQQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQQCMALSGCDAVMIGRGALNIPNLSRV
VKYNEPRMPWAQVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYSEATTLFQQIRALNNSPAIAQAIQAIKL

Sequences:

>Translated_316_residues
MGKNMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFQRICPELQNSSRTPSGTLVRIQLLGQYPQW
LAENALRAVELGSFGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEQKFEIADA
VQQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQQCMALSGCDAVMIGRGALNIPNLSRV
VKYNEPRMPWAQVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYSEATTLFQQIRALNNSPAIAQAIQAIKL
>Mature_315_residues
GKNMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFQRICPELQNSSRTPSGTLVRIQLLGQYPQWL
AENALRAVELGSFGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEQKFEIADAV
QQAGATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQQCMALSGCDAVMIGRGALNIPNLSRVV
KYNEPRMPWAQVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYSEATTLFQQIRALNNSPAIAQAIQAIKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=208, Percent_Identity=30.2884615384615, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=28.6245353159851, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=28.5123966942149, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI239788483, Length=177, Percent_Identity=31.638418079096, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=180, Percent_Identity=31.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=92.948717948718, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=28.6163522012579, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693211, Length=253, Percent_Identity=26.8774703557312, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=33.953488372093, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.7142857142857, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=220, Percent_Identity=26.3636363636364, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=199, Percent_Identity=29.1457286432161, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585320, Length=219, Percent_Identity=30.1369863013699, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=30.1675977653631, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=30.1675977653631, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35106; Mature: 34975

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFQRICPELQNS
CCCCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRTPSGTLVRIQLLGQYPQWLAENALRAVELGSFGVDLNCGCPSKTVNGSGGGATLLKDP
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCH
ELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEQKFEIADAVQQAGATELVVHGRTKEQGY
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
RAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQQCMALSGCDAVMIGRGALNIPNLSRV
CCCCCCHHHHHHHHHHEECEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
VKYNEPRMPWAQVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYSEATTLFQQIRA
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNNSPAIAQAIQAIKL
CCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GKNMRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFQRICPELQNS
CCCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRTPSGTLVRIQLLGQYPQWLAENALRAVELGSFGVDLNCGCPSKTVNGSGGGATLLKDP
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCH
ELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEQKFEIADAVQQAGATELVVHGRTKEQGY
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
RAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQQCMALSGCDAVMIGRGALNIPNLSRV
CCCCCCHHHHHHHHHHEECEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
VKYNEPRMPWAQVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYSEATTLFQQIRA
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNNSPAIAQAIQAIKL
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]