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The map label for this gene is wcaJ [H]

Identifier: 218549462

GI number: 218549462

Start: 2161267

End: 2162661

Strand: Reverse

Name: wcaJ [H]

Synonym: EFER_2131

Alternate gene names: 218549462

Gene position: 2162661-2161267 (Counterclockwise)

Preceding gene: 218549463

Following gene: 218549461

Centisome position: 47.13

GC content: 53.98

Gene sequence:

>1395_bases
ATGACAAATCTAAAAAAGCGCGAGCGAGCGAAAACCAATGCATCGTTAATCTCTATGGTGCAACGCTTTTCAGATATCAC
CATCATGTTTGCCGGGCTATGGCTGGTTTGCGAAGTCAGCGGACTGTCATTCCTCTACATGCACCTGTTGGTGGCGCTGA
TTACGCTGGTGGTGTTCCAGATGCTGGGCGGTATCACCGATTTTTATCGCTCATGGCGCGGTGTTCGGGCAACGACAGAA
TTTGCCCTGCTGCTGCAAAACTGGACCTTAAGCGTGATTTTTAGCGCCGGACTGGTGGCGTTCAACAATGATTTCGACAC
GCAACTGAAAATCTGGCTGGCGTGGTATGGGCTGACCAGCATCGGACTGGTGGTTTGCCGTTCGTGTATTCGCATTGGGG
CGGGCTGGCTGCGTAATCATGGCTATAACAAGCGCATGATCGCCGTAGCGGGGGATTTAGCCGCCGGACAAATGCTGATG
GAGAGTTTCCGTAATCAGCCGTGGTTAGGGTTTGAAGTGGTGGGCGTGTACCACGACCCGAAACCGGGCGGCGTTTCTAA
CGACTGGGCGGGCAATCTGCAACAGCTGGTCGAGGATGCAAAAGCGGGCAAGATTCACAACATCTATATCGCGATGCAAA
TGTGCGACGGCGCACGAGTGAAAAAACTGGTCCATCAACTGGCGGACACCACCTGTTCGGTGCTGCTGATCCCCGACGTC
TTTACCTTCAACATTCTCCATTCACGCCTCGAAGAGATGAACGGCGTTCCGGTGGTGCCGCTGTACGACACGCCACTTTC
CGGGGTTAACCGCCTGATAAAACGTGCGGAAGACATTGTGCTGGCAACGCTGATCTTGCTGCTGATCTCCCCGGTGCTGT
GCTGTATTGCGCTGGCGGTGAAACTCAGTTCACCAGGGCCGGTTATTTTCCGCCAGACTCGCTACGGCATGGATGGCAAG
CCGATCAAAGTGTGGAAGTTCCGTTCCATGAAAGTGATGGAGAACGACAAAGTCGTGACTCAGGCGACGCAGAACGATCC
GCGCGTCACCAAAGTGGGGAACTTTCTGCGCCGCACCTCGCTGGATGAATTGCCGCAGTTTATCAATGTGCTGACCGGGG
GGATGTCGATTGTGGGGCCACGTCCGCACGCGGTGGCGCATAACGAACAATATCGCCAGCTCATTGAAGGCTACATGCTG
CGCCACAAAGTGAAACCGGGCATTACCGGTTGGGCGCAGATTAACGGCTGGCGCGGCGAAACCGACACGCTGGAGAAAAT
GGAAAAACGCGTCGAGTTCGACCTTGAATACATCCGTGAATGGAGCGTCTGGTTCGATATCAAAATCGTTTTCCTGACGG
TATTCAAAGGCTTCGTTAACAAAGCGGCATATTGA

Upstream 100 bases:

>100_bases
GATGGAAGCGCGAACGCGAACTCTGCTGACGTTGCTGAACGAGTAATGTCGGATCTTCCCTTACCCCATTGCGGGTAAGG
GGCTAATAACAGGAACAACG

Downstream 100 bases:

>100_bases
TATGAGCTTACGTGAAAAAACCATCAGCGGCGCGAAGTGGTCGGCGATTGCTACGGTGATCATCATCGGCCTCGGGCTAG
TGCAGATGACCGTGCTGGCG

Product: UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQMLGGITDFYRSWRGVRATTE
FALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMIAVAGDLAAGQMLM
ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNIYIAMQMCDGARVKKLVHQLADTTCSVLLIPDV
FTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGK
PIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY

Sequences:

>Translated_464_residues
MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQMLGGITDFYRSWRGVRATTE
FALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMIAVAGDLAAGQMLM
ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNIYIAMQMCDGARVKKLVHQLADTTCSVLLIPDV
FTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGK
PIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY
>Mature_463_residues
TNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQMLGGITDFYRSWRGVRATTEF
ALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMIAVAGDLAAGQMLME
SFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNIYIAMQMCDGARVKKLVHQLADTTCSVLLIPDVF
TFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKP
IKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLR
HKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=464, Percent_Identity=98.9224137931034, Blast_Score=954, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52453; Mature: 52322

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS01047 HMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MLGGITDFYRSWRGVRATTEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEHHHHHHHHH
IGLVVCRSCIRIGAGWLRNHGYNKRMIAVAGDLAAGQMLMESFRNQPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSNDWAGNLQQLVEDAKAGKIHNIYIAMQMCDGARVKKLVHQLADTTCSVLLIPDV
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCH
FTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLCCIALAV
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTS
HCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MLGGITDFYRSWRGVRATTEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEHHHHHHHHH
IGLVVCRSCIRIGAGWLRNHGYNKRMIAVAGDLAAGQMLMESFRNQPWLGFEVVGVYHDP
HHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEECC
KPGGVSNDWAGNLQQLVEDAKAGKIHNIYIAMQMCDGARVKKLVHQLADTTCSVLLIPDV
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCH
FTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLCCIALAV
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTS
HCCCCCCEEEEECCCCCCCCCEEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCC
LDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPGITGWAQINGWRGE
HHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]