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The map label for this gene is pduW

Identifier: 218549359

GI number: 218549359

Start: 2075970

End: 2077184

Strand: Direct

Name: pduW

Synonym: EFER_2027

Alternate gene names: 218549359

Gene position: 2075970-2077184 (Clockwise)

Preceding gene: 218549358

Following gene: 218549363

Centisome position: 45.24

GC content: 47.41

Gene sequence:

>1215_bases
ATGTCACATAAAATCATGGCGATTAACGCGGGCAGCTCATCGCTTAAGTTTCAGTTACTGGCAATGCCGGAAGGCGAAAT
CTTATGCCAGGGAATAATTGAACGTATTGGTCTTGCGGATGCACGCCTTGTAGTCAAAACAGCGACCGAGAAATGGCAAG
AAATTACCCCTGTCGCCGATCATCGTGAAGCCGTCACTTTATTGCTTGAACAGCTTATTAATCGGAAGATTATTAATAGC
TTACATGATATCGATGCTACCGGGCATCGTGTAGCCCATGGGGGTGAGACGTTTAAGGATTCCGCGCTGGTGACAGATGA
TGTTATGGCAGAGATTGAACGTCTTGCGGAACTTGCACCTCTACATAATCCGGTCAACCTGTTGGGGATAAATATCTTCC
GCCAGTTGCTTCCCTCAGCCCCAACAATCGCCGTTTTCGATACAGCTTTTCATCAAACACTTGAAATGCCAGCTTATATT
TACCCTCTGCCCTGGCGCTACTACCACGAACTTGGAATTCGCCGCTACGGTTTTCATGGCACCAGCCATAAGTATGTCAG
TGGTAAATTAGCGGAAAAACTGGGCGTACCTCTCAGTGCATTGCGGGTAGTATGTTGTCATCTTGGTAACGGCAGCAGTG
TTTGTGCAATAAAAGGCGGAAAATCGGTCAATACGTCTATGGGATTTACGCCACAATCAGGCGTCATGATGGGGACTCGC
AGCGGTGATATTGATCCGTCAATTTTACCGTGGATTGCGTTGCGTGAAGGTAAAACACCGCAAGAGCTGAACCAACTCTT
AAATAATGAATCGGGGTTGTTAGGGGTTTCCGGGGTTTCACCCGATTTTCGTGATATTGAACAGGCTGCAGAAAATGGTA
ATCAGCAGGCGCAACTGGCACTCGCCCTCTTTGCTGAACGCATCCGCGCCACCATTGGCAGCTACGTCCTGCAAATGGGC
GGACTGGATGCCCTGATCTTTACTGGCGGTATCGGGGAAAACTCTGCCAGGGCGCGTGCAGCAATTTGCAACAATCTACA
TTTCCTTGGGTTGAGCATTGATGCCGATAAAAATCAGCACAATGCAACCTTTATACAGTCAGAACAGGCAATGGTTAAAG
TTGCAGTAATAAACACTAATGAAGAGTTAATGATTGCGCGTGATGTGATGCGTATTGCTTTGCCAGAACCGGCATTACAG
GAAGTGCTTGCATAA

Upstream 100 bases:

>100_bases
TGGCTCAAACAAGCCGGAGCACAGCAGATTTTCATTACCAGCGCAGTCGCCAAAAGTGGACTGGACGAAATATTTACTTA
TTTGAATGTAGAGGCTCTTC

Downstream 100 bases:

>100_bases
AAAAAAGTATGGTGAGGATCAGAACGATCCTCACCACTATAATCGCCATACTTCAAGTTGCATGTACTGCGTCTGCCTGC
AACTTGAATTATTCAGAGTC

Product: propionate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSHKIMAINAGSSSLKFQLLAMPEGEILCQGIIERIGLADARLVVKTATEKWQEITPVADHREAVTLLLEQLINRKIINS
LHDIDATGHRVAHGGETFKDSALVTDDVMAEIERLAELAPLHNPVNLLGINIFRQLLPSAPTIAVFDTAFHQTLEMPAYI
YPLPWRYYHELGIRRYGFHGTSHKYVSGKLAEKLGVPLSALRVVCCHLGNGSSVCAIKGGKSVNTSMGFTPQSGVMMGTR
SGDIDPSILPWIALREGKTPQELNQLLNNESGLLGVSGVSPDFRDIEQAAENGNQQAQLALALFAERIRATIGSYVLQMG
GLDALIFTGGIGENSARARAAICNNLHFLGLSIDADKNQHNATFIQSEQAMVKVAVINTNEELMIARDVMRIALPEPALQ
EVLA

Sequences:

>Translated_404_residues
MSHKIMAINAGSSSLKFQLLAMPEGEILCQGIIERIGLADARLVVKTATEKWQEITPVADHREAVTLLLEQLINRKIINS
LHDIDATGHRVAHGGETFKDSALVTDDVMAEIERLAELAPLHNPVNLLGINIFRQLLPSAPTIAVFDTAFHQTLEMPAYI
YPLPWRYYHELGIRRYGFHGTSHKYVSGKLAEKLGVPLSALRVVCCHLGNGSSVCAIKGGKSVNTSMGFTPQSGVMMGTR
SGDIDPSILPWIALREGKTPQELNQLLNNESGLLGVSGVSPDFRDIEQAAENGNQQAQLALALFAERIRATIGSYVLQMG
GLDALIFTGGIGENSARARAAICNNLHFLGLSIDADKNQHNATFIQSEQAMVKVAVINTNEELMIARDVMRIALPEPALQ
EVLA
>Mature_403_residues
SHKIMAINAGSSSLKFQLLAMPEGEILCQGIIERIGLADARLVVKTATEKWQEITPVADHREAVTLLLEQLINRKIINSL
HDIDATGHRVAHGGETFKDSALVTDDVMAEIERLAELAPLHNPVNLLGINIFRQLLPSAPTIAVFDTAFHQTLEMPAYIY
PLPWRYYHELGIRRYGFHGTSHKYVSGKLAEKLGVPLSALRVVCCHLGNGSSVCAIKGGKSVNTSMGFTPQSGVMMGTRS
GDIDPSILPWIALREGKTPQELNQLLNNESGLLGVSGVSPDFRDIEQAAENGNQQAQLALALFAERIRATIGSYVLQMGG
LDALIFTGGIGENSARARAAICNNLHFLGLSIDADKNQHNATFIQSEQAMVKVAVINTNEELMIARDVMRIALPEPALQE
VLA

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family. PduW subfamily

Homologues:

Organism=Escherichia coli, GI145693184, Length=394, Percent_Identity=44.9238578680203, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=41.645885286783, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDUW_ESCF3 (B7LTX5)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002383150.1
- EnsemblBacteria:   EBESCT00000123473
- GeneID:   7121263
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_2027
- GeneTree:   EBGT00050000009666
- HOGENOM:   HBG285863
- ProtClustDB:   PRK12397
- BioCyc:   EFER585054:EFER_2027-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01882
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.15

Molecular weight: Translated: 43912; Mature: 43781

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKIMAINAGSSSLKFQLLAMPEGEILCQGIIERIGLADARLVVKTATEKWQEITPVAD
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHH
HREAVTLLLEQLINRKIINSLHDIDATGHRVAHGGETFKDSALVTDDVMAEIERLAELAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
LHNPVNLLGINIFRQLLPSAPTIAVFDTAFHQTLEMPAYIYPLPWRYYHELGIRRYGFHG
CCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEEECCCHHHHHHHCCEEECCCC
TSHKYVSGKLAEKLGVPLSALRVVCCHLGNGSSVCAIKGGKSVNTSMGFTPQSGVMMGTR
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC
SGDIDPSILPWIALREGKTPQELNQLLNNESGLLGVSGVSPDFRDIEQAAENGNQQAQLA
CCCCCCCHHCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHH
LALFAERIRATIGSYVLQMGGLDALIFTGGIGENSARARAAICNNLHFLGLSIDADKNQH
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCEEEEEEEEECCCCCC
NATFIQSEQAMVKVAVINTNEELMIARDVMRIALPEPALQEVLA
CCEEEECCCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SHKIMAINAGSSSLKFQLLAMPEGEILCQGIIERIGLADARLVVKTATEKWQEITPVAD
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHH
HREAVTLLLEQLINRKIINSLHDIDATGHRVAHGGETFKDSALVTDDVMAEIERLAELAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
LHNPVNLLGINIFRQLLPSAPTIAVFDTAFHQTLEMPAYIYPLPWRYYHELGIRRYGFHG
CCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEEECCCHHHHHHHCCEEECCCC
TSHKYVSGKLAEKLGVPLSALRVVCCHLGNGSSVCAIKGGKSVNTSMGFTPQSGVMMGTR
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECC
SGDIDPSILPWIALREGKTPQELNQLLNNESGLLGVSGVSPDFRDIEQAAENGNQQAQLA
CCCCCCCHHCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHH
LALFAERIRATIGSYVLQMGGLDALIFTGGIGENSARARAAICNNLHFLGLSIDADKNQH
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCEEEEEEEEECCCCCC
NATFIQSEQAMVKVAVINTNEELMIARDVMRIALPEPALQEVLA
CCEEEECCCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA