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The map label for this gene is shiA [H]

Identifier: 218549321

GI number: 218549321

Start: 2039566

End: 2040870

Strand: Reverse

Name: shiA [H]

Synonym: EFER_1984

Alternate gene names: 218549321

Gene position: 2040870-2039566 (Counterclockwise)

Preceding gene: 218549322

Following gene: 218549320

Centisome position: 44.48

GC content: 49.89

Gene sequence:

>1305_bases
ATGGACTCCACACTTATTTCAACGCCTCCAAAAGAGGACACTCCATCGCTTACCCGTGCTCGTCGTGCTGCTTTCGGTAG
TTTTGCCGGAGCAGTGGTTGACTGGTACGATTTTCTGCTTTATGGCATCACTGCCGCGTTAGTGTTTAACAGTGAATTCT
TCCCACAAATTAGTCCGGCCATGGGCACACTGGCTGCTTTTGCAACATTTGGCGTCGGCTTTTTGTTTCGTCCACTGGGT
GGCGTGATTTTTGGTCATTTTGGCGACCGCCTTGGACGCAAACGGATGCTGATGCTTACCGTCTGGATGATGGGGATTGC
GACGGCACTGATTGGCATTCTGCCATCGTTTGCTGCAATTGGCTGGTGGGCTCCGGTTCTGCTTGTTACCTTACGGGCAA
TCCAGGGATTTGCCGTTGGTGGGGAGTGGGGCGGTGCGGCGTTGCTGTCGGTCGAAAGCGCACCTGAAAACAAAAAAGCC
TTTTATAGCAGTGGGGTACAGGTCGGTTATGGTGTCGGTCTTTTGCTCTCTACCGGGCTGGTCTCGCTGATCAGCATGAT
GACCACAGATGAACAGTTTTTAAGCTGGGGCTGGCGTATTCCGTTTTTATTCAGCATCGTGCTGGTGCTTGGCGCATTAT
GGATTCGTAACGGAATGGAAGAATCTGCCGAGTTTGAAAAGCAGCAACAAAAAACATCACCAAAAACACGTTTACCGGTG
TTGGAAGCACTTAGCGGCCATCCCGGCGCTTTTTTGAAAATCATCGGCTTGCGTTTGTGTGAACTTTTGACCATGTATAT
CGTCACTGCGTTTGCACTGAACTACTCGACACAAAATCTCGGGCTTCCACGCGAACTCTTTCTGAATATCGGCCTGCTGG
TGGGTGGACTAAGTTGCCTGACCATTCCGTGCTTTGCATGGCTTGCAGATCGTTTTGGTCGTCGTCGCGTTTATATTACG
GGAGCGTTAATTGGCACACTCAGTGCTTTCCCGTTTTTTATAGCGTTGGAATCCCAATCTATTTTTTGGATCGTGTTCTT
CTCAATAATGCTGGCGAATATTGCCCATGATATGGTGGTTTGTGTGCAGCAACCGATGTTTACCAGCATGTTTGGCGCGA
GTTACCGCTACAGTGGTGCTGGTGTGGGGTATCAGGTCGCAAGTGTGGTGGGGGGAGGTTTTACACCATTCATTGCCGCA
GCTCTTGTCACATTTTCTGCGGGTGACTGGCGGAGCGTAGCTATTTACCTGCTTGTTGGTTGCCTTATCTCAGCGGCGAC
AGCATCATTAATGAAAGATAAGTAA

Upstream 100 bases:

>100_bases
GCGCGATTTGTCAGCAATCTGAGTCGGTTATACAACGGCTGCAATGTGAGTAACCCATTTTTATACTTATAACTAAAAAA
CCAGTACTGACAGGACAATC

Downstream 100 bases:

>100_bases
CTGTTATCACATTAATTGCGAAGGTGTTCCGGCCTTCGCATATCTCTTCTTTAAGTCTCACTTTAGTTTCACATTACTGT
GACATACTATCGAAAGAGCA

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDSTLISTPPKEDTPSLTRARRAAFGSFAGAVVDWYDFLLYGITAALVFNSEFFPQISPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFAAIGWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWIRNGMEESAEFEKQQQKTSPKTRLPV
LEALSGHPGAFLKIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYIT
GALIGTLSAFPFFIALESQSIFWIVFFSIMLANIAHDMVVCVQQPMFTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAA
ALVTFSAGDWRSVAIYLLVGCLISAATASLMKDK

Sequences:

>Translated_434_residues
MDSTLISTPPKEDTPSLTRARRAAFGSFAGAVVDWYDFLLYGITAALVFNSEFFPQISPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFAAIGWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWIRNGMEESAEFEKQQQKTSPKTRLPV
LEALSGHPGAFLKIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYIT
GALIGTLSAFPFFIALESQSIFWIVFFSIMLANIAHDMVVCVQQPMFTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAA
ALVTFSAGDWRSVAIYLLVGCLISAATASLMKDK
>Mature_434_residues
MDSTLISTPPKEDTPSLTRARRAAFGSFAGAVVDWYDFLLYGITAALVFNSEFFPQISPAMGTLAAFATFGVGFLFRPLG
GVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFAAIGWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWIRNGMEESAEFEKQQQKTSPKTRLPV
LEALSGHPGAFLKIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYIT
GALIGTLSAFPFFIALESQSIFWIVFFSIMLANIAHDMVVCVQQPMFTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAA
ALVTFSAGDWRSVAIYLLVGCLISAATASLMKDK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=90.8045977011494, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=32.6340326340326, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789941, Length=400, Percent_Identity=38.25, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788942, Length=396, Percent_Identity=31.5656565656566, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082404, Length=391, Percent_Identity=25.8312020460358, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082231, Length=439, Percent_Identity=21.8678815489749, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46961; Mature: 46961

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTLISTPPKEDTPSLTRARRAAFGSFAGAVVDWYDFLLYGITAALVFNSEFFPQISPA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPENKKAFYSSGVQVGYGVGLLLSTGL
HHHHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
VSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWIRNGMEESAEFEKQQQKTSPKTRLPV
HHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCHH
LEALSGHPGAFLKIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
TIPCFAWLADRFGRRRVYITGALIGTLSAFPFFIALESQSIFWIVFFSIMLANIAHDMVV
HHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CVQQPMFTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALVTFSAGDWRSVAIYLLVG
HHCCHHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
CLISAATASLMKDK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MDSTLISTPPKEDTPSLTRARRAAFGSFAGAVVDWYDFLLYGITAALVFNSEFFPQISPA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
MGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPENKKAFYSSGVQVGYGVGLLLSTGL
HHHHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
VSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWIRNGMEESAEFEKQQQKTSPKTRLPV
HHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCHH
LEALSGHPGAFLKIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
TIPCFAWLADRFGRRRVYITGALIGTLSAFPFFIALESQSIFWIVFFSIMLANIAHDMVV
HHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
CVQQPMFTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALVTFSAGDWRSVAIYLLVG
HHCCHHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
CLISAATASLMKDK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]