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The map label for this gene is sapA [H]

Identifier: 218549012

GI number: 218549012

Start: 1705827

End: 1707470

Strand: Direct

Name: sapA [H]

Synonym: EFER_1660

Alternate gene names: 218549012

Gene position: 1705827-1707470 (Clockwise)

Preceding gene: 218549011

Following gene: 218549013

Centisome position: 37.17

GC content: 49.21

Gene sequence:

>1644_bases
ATGCGCCTGGTTATATCGTCCCTGATCGTAATCGCTGGACTCCTGAGCGGAAACGCCGCAGCTGCGCCTGAACAGACTCC
CCCTGCCGATATTCGTGACAGCGGTTTTGTCTATTGCGTTAGCGGGCAGGTCAACACGTTTAACCCATCAAAAGCGAGTA
GCGGGTTGATTGTTGATACCCTCGCAGCTCAATTTTATGACCGTCTGCTGGATGTAGATCCCTATACTTATCGACTGATG
CCTGAGCTGGCCGAAAGTTGGGATGTACTGGATAACGGTGCAACTTACCGCTTTCATCTGCGCCGTGATGTCCCGTTTCA
GAAAACTAACTGGTTTACTCCAACACGTAATCTGAATGCCGATGACGTAGTGTTTACCTTTCAACGCATTTTTGACCGTA
ATAATCCGTGGCATAACGTTAACGGCAATAACTATCCCTACTTTGATAGCCTGCAATTTGCCGATAACGTTAAAAGTGTG
CGCAAAATTGACAACCACACTGTTGAGTTTCGTCTTGCGCAACCAGACGCCTCTTTCTTATGGCACCTTGCGACACACTA
TGCTTCGGTGATGTCAGCAGAATACGCCACCGAACTGGCAAAAGAAGATCGTGAAGAGTTACTTGACCGCCAACCTGTGG
GTACCGGGCCATACAAACTTTCTGAATATCGTGCCGGGCAGTATATTCGTCTGCAACGTCATGATGCATTCTGGCGCGGT
AAACCGTTGACGCCACAAGTCGTTGTGGATCTGGGCTCCGGCGGTACGGGGCGTTTGTCTAAACTGCTCACTGGTGAATG
TGATGTTCTCGCCTGGCCTGCTGCCAGTCAGTTAACTATTTTGCGTGACGATCCACGTTTGCGCTTGACGCTGCGCCCAG
GAATGAATATCGCTTATCTGGCGTTTAATACCGCCAAGCCACCGCTCAATAATCCTGCTGTTCGTCACGCTCTTGCACTG
GCGATCAATAACCAGCGTCTTATGCAGTCTATTTATTATGGAACGGCAGAAACGGCTGCATCTATTTTACCGCGTGCCTC
CTGGGCCTATGACAACGAGGCTAAAATTACAGAATACAATCCGGCGAAGTCGCGTGAGCAATTAAAAGCGCTGGGGCTGG
AAAAGTTATCGCTGAAGCTGTGGGTACCAACACGTTCCCAGGCCTGGAACCCAAGTCCGCTTAAAATGGCGGAGTTGATT
CAGGCTGATATGGCACAAGTGGGTGTCAAAGTGACAATTGTTCCGGTTGAAGGTCGCTTTCAGGAGGCACGGTTAATGGA
TATGAACCATGATCTGACGTTGTCTGGTTGGGCGACAGACAGTAATGACCCTGACAGTTTCTTCCGGCCATTGCTCAGTT
GCGCCGCCATTAAGTCGCAGACTAACTTTGCACACTGGTGCGATCCGCAATTTGATGAAGTGTTGCGTAAAGCGCTCTCT
TCGCAACAATTGTCTGCGCGTATCGAAGCCTATGATGAAGCTCAGGCAATTTTGGCTAAGGAATTGCCGATTCTGCCATT
GGCATCCTCATTGCGTTTACAGGCTTACCGCTACGATATTAAAGGACTAGTGTTAAGCCCTTTTGGTAATGCTTCATTTG
CCGGGGTTTATCGTGAAAAAGCGAAAGAGGTGAAAAAACCATGA

Upstream 100 bases:

>100_bases
AGTTTGCTGACATTTGCGCCAGTTATCGGTAGACATTTTTACCTGGCAGGCCATAGTGCGATACACTTTGCAGATCGAAA
CTAAAAACCTCAAAAAAATT

Downstream 100 bases:

>100_bases
TTATTTTCACCCTGCGACGCATTCTACTCTTGCTGGTCACTCTTTTCTTTCTGACCTTTGTGGGCTTTAGCCTTAATTAC
TTTACACCCCACGCACCGTT

Product: antimicrobial peptide ABC transporter periplasmic-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MRLVISSLIVIAGLLSGNAAAAPEQTPPADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWDVLDNGATYRFHLRRDVPFQKTNWFTPTRNLNADDVVFTFQRIFDRNNPWHNVNGNNYPYFDSLQFADNVKSV
RKIDNHTVEFRLAQPDASFLWHLATHYASVMSAEYATELAKEDREELLDRQPVGTGPYKLSEYRAGQYIRLQRHDAFWRG
KPLTPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLEKLSLKLWVPTRSQAWNPSPLKMAELI
QADMAQVGVKVTIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAIKSQTNFAHWCDPQFDEVLRKALS
SQQLSARIEAYDEAQAILAKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKAKEVKKP

Sequences:

>Translated_547_residues
MRLVISSLIVIAGLLSGNAAAAPEQTPPADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWDVLDNGATYRFHLRRDVPFQKTNWFTPTRNLNADDVVFTFQRIFDRNNPWHNVNGNNYPYFDSLQFADNVKSV
RKIDNHTVEFRLAQPDASFLWHLATHYASVMSAEYATELAKEDREELLDRQPVGTGPYKLSEYRAGQYIRLQRHDAFWRG
KPLTPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLEKLSLKLWVPTRSQAWNPSPLKMAELI
QADMAQVGVKVTIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAIKSQTNFAHWCDPQFDEVLRKALS
SQQLSARIEAYDEAQAILAKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKAKEVKKP
>Mature_547_residues
MRLVISSLIVIAGLLSGNAAAAPEQTPPADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWDVLDNGATYRFHLRRDVPFQKTNWFTPTRNLNADDVVFTFQRIFDRNNPWHNVNGNNYPYFDSLQFADNVKSV
RKIDNHTVEFRLAQPDASFLWHLATHYASVMSAEYATELAKEDREELLDRQPVGTGPYKLSEYRAGQYIRLQRHDAFWRG
KPLTPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLEKLSLKLWVPTRSQAWNPSPLKMAELI
QADMAQVGVKVTIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAIKSQTNFAHWCDPQFDEVLRKALS
SQQLSARIEAYDEAQAILAKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKAKEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=547, Percent_Identity=92.3217550274223, Blast_Score=1035, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=515, Percent_Identity=36.1165048543689, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1787052, Length=510, Percent_Identity=27.4509803921569, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787762, Length=467, Percent_Identity=23.3404710920771, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=25.4681647940075, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=25.3012048192771, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=23.21083172147, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61419; Mature: 61419

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVISSLIVIAGLLSGNAAAAPEQTPPADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWDVLDNGATYRFHLRRDVPFQKTNWFTPTRNLNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGNNYPYFDSLQFADNVKSVRKIDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVMSAEYATELAKEDREELLDRQPVGTGPYKLSEYRAGQYIRLQRHDAFWRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLTPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYL
CCCCCEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECC
PAKSREQLKALGLEKLSLKLWVPTRSQAWNPSPLKMAELIQADMAQVGVKVTIVPVEGRF
CCHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAIKSQTNFAHWCDPQFDEVLRKALS
CCHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SQQLSARIEAYDEAQAILAKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCHHHHHHHH
AKEVKKP
HHHHCCC
>Mature Secondary Structure
MRLVISSLIVIAGLLSGNAAAAPEQTPPADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWDVLDNGATYRFHLRRDVPFQKTNWFTPTRNLNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGNNYPYFDSLQFADNVKSVRKIDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVMSAEYATELAKEDREELLDRQPVGTGPYKLSEYRAGQYIRLQRHDAFWRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLTPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYL
CCCCCEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECC
PAKSREQLKALGLEKLSLKLWVPTRSQAWNPSPLKMAELIQADMAQVGVKVTIVPVEGRF
CCHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAIKSQTNFAHWCDPQFDEVLRKALS
CCHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SQQLSARIEAYDEAQAILAKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCHHHHHHHH
AKEVKKP
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]