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The map label for this gene is gadC [H]

Identifier: 218548930

GI number: 218548930

Start: 1615255

End: 1616790

Strand: Direct

Name: gadC [H]

Synonym: EFER_1577

Alternate gene names: 218548930

Gene position: 1615255-1616790 (Clockwise)

Preceding gene: 218548929

Following gene: 218548931

Centisome position: 35.2

GC content: 48.31

Gene sequence:

>1536_bases
ATGGCTACATCAGTACAGACAGGTAAAGCTAAGCAGCTAACATTGCTTGGATTCTTCGCTATTACGGCGTCGATGGTTAT
GGCTGTTTATGAATACCCAACTTTCGCAACTTCCGGTTTTTCATTAGTCTTTTTCCTGCTATTAGGCGGGATTTTGTGGT
TTATTCCCGTGGGCCTGTGTGCTGCTGAAATGGCTACCGTGGAAGGCTGGGAGGAAGGTGGTGTATTCGCATGGGTATCA
AATACCCTGGGGCTTAAATGGGGATTTGCAGCTATCTCCTTTGGCTATCTGCAAATCGCAATTGGCTTTATTCCGATGCT
CTATTTCGTATTAGGTGCGCTCTCCTACATCCTGAAATGGCCAGCACTAAACGAAGACCCGGTCACGAAAACCATTGCCG
CACTCATCATTCTTTGGGCGCTGGCATTGACCCAGTTCGGCGGCACCAAATATACGGCGCGGATTGCCAAAGTTGGTTTC
TTCGCCGGTATCTTGCTGCCTGCATTTATTTTGATCGCCTTAGCGGCAATCTATCTGCACTCCGGTGCGCCGATTGCTAT
CGAAATGGATGCGAAAACCTTCTTCCCTGATTTCACAAAAGTTGGCACGCTGGTTGTGTTTGTCGCCTTTATCCTGAGTT
ATATGGGGGTAGAAGCTTCCGCAACCCACGTCAATGAAATGAGCAACCCAGGACGAGACTATCCGTTAGCCATGTTACTG
CTGATGGTGGCAGCAATCTGCCTAAGCTCTGTCGGTGGTTTGTCCATTGCGATGGTGATTCCAGGGAATGAAATCAACTT
ATCCGCAGGTGTTATGCAAACTTTCACCGTTCTGATGTCACATGTGGCACCGGAAATTGAGTGGACAGTTCGTGTCATCT
CAGCCCTGCTGCTGCTGGGTGTTCTGGCGGAAATCGCTTCCTGGATTGTTGGCCCTTCTCGCGGTATGTATGTGACTGCG
CAGAAAAACCTGCTACCCGCTGCGTTCGCCAAAATGAATAAAAATGGCGTGCCAGTAACGCTGGTTATCTCTCAGTTGGT
AATTACTTCCATCGCGTTGATTATCCTCACCAATACAGGTGGTGGTAACAACATGTCCTTCCTGATCGCGCTGGCGTTGA
CCGTGGTAATTTATCTGTGTGCTTATTTCATGCTGTTTATTGGCTACATTGTGTTAGTTCTTAAACACCCTGATTTAAAA
CGCACATTTAATATCCCTGGCGGTAAAGGTGTGAAACTGGTGGTCGCGATTGTGGGTCTGCTGACTTCAATTATGGCGTT
CATTGTTTCCTTCCTGCCACCGGATAACATCCAGGGTGACTCGACCGATATGTACGTTGAATTGTTGGTAGTAAGTTTCC
TGGTGGTACTCGCTCTGCCGTTTATCCTCTATGCGCTGCATGACCGCAAACACAAAGTGGATACTGGTGTGGCACTGGAA
CCTATCAACAGTCAGAACGCACCGAAGGGGCATTTCTTCCTGCACCCACGTGCCCGTTCCCCGCACTATGTGGTGATCGA
TAACAAAAAACACTAA

Upstream 100 bases:

>100_bases
AGTCTGTTCACTGGCATTAGCAATGGAAAATGTTGCTCTGAACGGCGTTCAGGACAAGACAGGTGCGGTTCCGACAGGAC
AACCGTTTTAGGGGGATAAT

Downstream 100 bases:

>100_bases
CCCTGTTTAAGTGTCGTAACACAACCCAGAACGGGAGTGATGAATTTGATCACTCCCGTCTTTTTTGTAACCGTTGGTTG
TATTGACTTACCCTGGATAA

Product: glutamate:gamma-aminobutyric acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: Extreme acid sensitivity protein [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVEGWEEGGVFAWVS
NTLGLKWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPVTKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPIAIEMDAKTFFPDFTKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYALHDRKHKVDTGVALE
PINSQNAPKGHFFLHPRARSPHYVVIDNKKH

Sequences:

>Translated_511_residues
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVEGWEEGGVFAWVS
NTLGLKWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPVTKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPIAIEMDAKTFFPDFTKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYALHDRKHKVDTGVALE
PINSQNAPKGHFFLHPRARSPHYVVIDNKKH
>Mature_510_residues
ATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVEGWEEGGVFAWVSN
TLGLKWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPVTKTIAALIILWALALTQFGGTKYTARIAKVGFF
AGILLPAFILIALAAIYLHSGAPIAIEMDAKTFFPDFTKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLLL
MVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQ
KNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKR
TFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYALHDRKHKVDTGVALEP
INSQNAPKGHFFLHPRARSPHYVVIDNKKH

Specific function: Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by gadA and gadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=511, Percent_Identity=96.8688845401174, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI87082380, Length=436, Percent_Identity=23.8532110091743, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081798, Length=402, Percent_Identity=24.1293532338308, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 55139; Mature: 55007

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVEGWEEGGVFAWVSNTLGLKWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPVTKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPIAIEMDAKTFFPDFTKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYALHDRKHKVDTGVALE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
PINSQNAPKGHFFLHPRARSPHYVVIDNKKH
CCCCCCCCCCEEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure 
ATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVEGWEEGGVFAWVSNTLGLKWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPVTKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPIAIEMDAKTFFPDFTKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYALHDRKHKVDTGVALE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
PINSQNAPKGHFFLHPRARSPHYVVIDNKKH
CCCCCCCCCCEEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]