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The map label for this gene is ydeH [H]

Identifier: 218548895

GI number: 218548895

Start: 1573742

End: 1574707

Strand: Direct

Name: ydeH [H]

Synonym: EFER_1540

Alternate gene names: 218548895

Gene position: 1573742-1574707 (Clockwise)

Preceding gene: 218548894

Following gene: 218548897

Centisome position: 34.3

GC content: 45.24

Gene sequence:

>966_bases
TTGACAGGACATGATGAAGAAGGAATGGCGATGATTAAGAAAACGGCTCAAATTGATGCAATCCTGTTAGATCTCAATAA
GTCTATCGATGCGCATTATCAGTGGCTGGTGAAAATGTTTCGCTGTGTTGTCGCAAAGGACATTTCGCACCCGGAACTCT
CTGATGCTCATTCCCATTTTCTCTGTCGTTTTGGACAATGGCTGAACGCACATCTGGTGATGACAGAGGAAGAAATAGAA
TATGTACGTTTGATAAGCCTGGCACATGAGGAAATGCACCTGCAGGGACGTGAATTATTGACCTCTATTGCCGAAAAACG
TTGGCAGTCAACCGATTTTGACCGCTTTCAAAATACCTTGCTGTCTTTTACTTCGGCGGTCATGGACTACAAAATATATC
TTCTGAATATCCGCAGTAATATGGATATCCTTACTGGTTTACCAGGTCGACGAGTACTTGATGAATCCTTCGATCGCCAG
TTACTCAACGCCGAACCTGAAAAGTTTTATTTGCTATTGCTGGATATCGATCGCTTTAAGAGTGTTAACGATACGCTGGG
GCATTTAATTGGTGATGTGGTTTTACGAACTCTTGCAGAAAATCTCGCCACCTGGATGCGGCACAATGAATCGGTCTATC
GCTATGGTGGCGAGGAGTTCGTAATTATGATCAAGGCGTCTACAGATGTAGACGCCTGTATGGCGGGGCTGCGTATTTGT
GAATTGATTGCAGGTAATCAGATAGCGTATCCGCAAGGTGAACTTCACATTACTGCAACGGCAGGAATTAGCCGGGCAAA
ACCAGGTGAAACTCTTGACGTCGTCATGGGGCGGGCAGACCGTGCTATGTATCAGGGAAAGCTGACCGGACGTAATCGCT
GTATGTTTATGGATGAAAATGAGCAGATCAGGCACGTAGCTGCCGCAGCAACAGAACTGGATAACCCTTGTTTAACTGTC
ATTTAG

Upstream 100 bases:

>100_bases
AATACCCCATGCATATTTCAATGAGTTAATTTGCTCAGTTTATTAAATATCTTAACTATGCCTAAATTGACATAAGTTTT
CTTTTATCTCGCTAAAATCT

Downstream 100 bases:

>100_bases
CTTAAAAGCAGTAAAATTCTTAATGAGTCGTTATCGGGTACAAAATGCCTGGTAGCGTAAAAAGCAAAACACATATCGCG
ACTCTCCATAGGTCGAGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTGHDEEGMAMIKKTAQIDAILLDLNKSIDAHYQWLVKMFRCVVAKDISHPELSDAHSHFLCRFGQWLNAHLVMTEEEIE
YVRLISLAHEEMHLQGRELLTSIAEKRWQSTDFDRFQNTLLSFTSAVMDYKIYLLNIRSNMDILTGLPGRRVLDESFDRQ
LLNAEPEKFYLLLLDIDRFKSVNDTLGHLIGDVVLRTLAENLATWMRHNESVYRYGGEEFVIMIKASTDVDACMAGLRIC
ELIAGNQIAYPQGELHITATAGISRAKPGETLDVVMGRADRAMYQGKLTGRNRCMFMDENEQIRHVAAAATELDNPCLTV
I

Sequences:

>Translated_321_residues
MTGHDEEGMAMIKKTAQIDAILLDLNKSIDAHYQWLVKMFRCVVAKDISHPELSDAHSHFLCRFGQWLNAHLVMTEEEIE
YVRLISLAHEEMHLQGRELLTSIAEKRWQSTDFDRFQNTLLSFTSAVMDYKIYLLNIRSNMDILTGLPGRRVLDESFDRQ
LLNAEPEKFYLLLLDIDRFKSVNDTLGHLIGDVVLRTLAENLATWMRHNESVYRYGGEEFVIMIKASTDVDACMAGLRIC
ELIAGNQIAYPQGELHITATAGISRAKPGETLDVVMGRADRAMYQGKLTGRNRCMFMDENEQIRHVAAAATELDNPCLTV
I
>Mature_320_residues
TGHDEEGMAMIKKTAQIDAILLDLNKSIDAHYQWLVKMFRCVVAKDISHPELSDAHSHFLCRFGQWLNAHLVMTEEEIEY
VRLISLAHEEMHLQGRELLTSIAEKRWQSTDFDRFQNTLLSFTSAVMDYKIYLLNIRSNMDILTGLPGRRVLDESFDRQL
LNAEPEKFYLLLLDIDRFKSVNDTLGHLIGDVVLRTLAENLATWMRHNESVYRYGGEEFVIMIKASTDVDACMAGLRICE
LIAGNQIAYPQGELHITATAGISRAKPGETLDVVMGRADRAMYQGKLTGRNRCMFMDENEQIRHVAAAATELDNPCLTVI

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=296, Percent_Identity=64.8648648648649, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=31.4465408805031, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=30.2325581395349, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=189, Percent_Identity=28.042328042328, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=35.625, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787056, Length=203, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=188, Percent_Identity=30.8510638297872, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=31.0975609756098, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=31.547619047619, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=30, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36555; Mature: 36423

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHDEEGMAMIKKTAQIDAILLDLNKSIDAHYQWLVKMFRCVVAKDISHPELSDAHSHF
CCCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LCRFGQWLNAHLVMTEEEIEYVRLISLAHEEMHLQGRELLTSIAEKRWQSTDFDRFQNTL
HHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSFTSAVMDYKIYLLNIRSNMDILTGLPGRRVLDESFDRQLLNAEPEKFYLLLLDIDRFK
HHHHHHHHHEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHH
SVNDTLGHLIGDVVLRTLAENLATWMRHNESVYRYGGEEFVIMIKASTDVDACMAGLRIC
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECCCHHHHHHHHHHH
ELIAGNQIAYPQGELHITATAGISRAKPGETLDVVMGRADRAMYQGKLTGRNRCMFMDEN
HHHCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCEEEEECCC
EQIRHVAAAATELDNPCLTVI
HHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
TGHDEEGMAMIKKTAQIDAILLDLNKSIDAHYQWLVKMFRCVVAKDISHPELSDAHSHF
CCCCCCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LCRFGQWLNAHLVMTEEEIEYVRLISLAHEEMHLQGRELLTSIAEKRWQSTDFDRFQNTL
HHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSFTSAVMDYKIYLLNIRSNMDILTGLPGRRVLDESFDRQLLNAEPEKFYLLLLDIDRFK
HHHHHHHHHEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHH
SVNDTLGHLIGDVVLRTLAENLATWMRHNESVYRYGGEEFVIMIKASTDVDACMAGLRIC
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECCCHHHHHHHHHHH
ELIAGNQIAYPQGELHITATAGISRAKPGETLDVVMGRADRAMYQGKLTGRNRCMFMDEN
HHHCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCEEEEECCC
EQIRHVAAAATELDNPCLTVI
HHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]