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The map label for this gene is ydfI [H]

Identifier: 218548888

GI number: 218548888

Start: 1566699

End: 1568186

Strand: Direct

Name: ydfI [H]

Synonym: EFER_1533

Alternate gene names: 218548888

Gene position: 1566699-1568186 (Clockwise)

Preceding gene: 218548887

Following gene: 218548892

Centisome position: 34.14

GC content: 48.92

Gene sequence:

>1488_bases
TTGGAATTAACAGGTCTTCTTATGGAAAACAGACTATTAAACGCCAGGGCTACACTTCCCGATTATGACCGCTCAAAACT
TGTTCCACGGATTGTTCATTTAGGATTTGGCGCTTTTCACCGGGCGCATCAGGCCGTATATACCGATATTCTGGCGAGTG
AACACGGTAGTGACTGGGGATTTCATGAAGTCAACCTGATCGGTGGTGAGCAGCAGATAGCAGATCTTAAACAACAGGAT
AACCTGTATACCGTGGCTGAAATGTCTGCTGACAACTGGACTGCACGGGTTGTTGGCGTTGTTAAAAACGCACTTCATGC
ACAGGTAGATGGCCTGGAAAGTGTGCTGGCCGTGATGTGTGAACCACAAGTAGCGATTGTGTCACTGACCATTACCGAGA
AAGGTTATTGTCATTCCCCGGCAACAGGGGAATTAATGCTGGATCACCCGATGATCGTGGCTGATCTGCATAACCCGACA
CAACCGAAAACGGCGCCAGGCATTGTGGTCGAAGCACTGGCACGCCGTAAATCTGCAGGGCTGTCTGCTTTTACGGTGAT
GTCCTGTGACAATATGCCAGAAAATGGTCATGTGATGCGCAATGTTATCCTCACTTATGCCAGAGCATTGAACAGTGAGC
TTGCCAGATGGATTGAGCAACATGTCACTTTCCCATCGACCATGGTTGACCGTATTGTTCCGGCAGTTACTCACGAGAGT
CTGGACAAAATAACGCAGTTAACGGGGGTCAGTGATCCGGCTGCTGTTGCCTGCGAACCTTTCCGCCAGTGGGTGATCGA
AGATAATTTTGTTGCCGGGCGTCCTGGATGGGAAAAAGCAGGGGCAGAACTGGTATCCAATGTTCTTCCTTATGAAGAAA
TGAAACTGCGTATGCTTAACGGTAGCCACTCTTTCCTGGCGTATCTTGGGTATCTGGCAGGTTATCAGCATATTAACGAA
TGTATGGCTGATGAGAATTACCGCCTGGCGGCACATTCACTTATGCTCCATGAGCAAGCGCCGACGTTAAAAGTACAGGG
TGTTGATTTAGGTAAGTACGCGGATTCCTTAATTGCGCGTTACAGCAATCCGGCATTACGCCATCGGACATGGCAGATTG
CCATGGATGGCAGCCAGAAGCTGCCGCAGAGAATGCTGGATTCAGTACGCTGGCATCTTGCCCATAATAGTGAATTTGAT
TTACTGGCGTTAGGTGTTGCAGCATGGATGCGCTATGTCGGCGGTGTGGACGAGCAGGGAAATGCGATTGAAGTGAGTGA
TCCGCTATTACCGTTGCTGCAGAAAGCGGTGGCTGGCAGTAAAGAAGGTGAAGAACGCGTTAAGGCCTTGTTTGCGATAG
AAGCGATCTTTGGTGCGGAATTACCAAAAGTAACAGCTTTCACTGCTAAAGTTAGCGCGGCATATCAATTGCTTCTGGCA
GAAGGTGCAAAGGTCGCGGTGGCAAAATACGCGGCAAAACTAAAGTAA

Upstream 100 bases:

>100_bases
GCAGATCGCGTTGTTTTTACCTTCGCTATTGATGTAAGGCCAGAACTCACCGCTACGCTATTCCTTGCGGATAAGTAGCG
CTTTTTTATTCACACTATGA

Downstream 100 bases:

>100_bases
TTTGAACGACATATCAGGCCTGCAAACTCGTGAAAATTGATTGAAATAAAAGAGTTTGTAGGCCGGATAAGAAACTGAAA
TCACGCCATTAGTTCATGCC

Product: mannonate dehydrogenase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MELTGLLMENRLLNARATLPDYDRSKLVPRIVHLGFGAFHRAHQAVYTDILASEHGSDWGFHEVNLIGGEQQIADLKQQD
NLYTVAEMSADNWTARVVGVVKNALHAQVDGLESVLAVMCEPQVAIVSLTITEKGYCHSPATGELMLDHPMIVADLHNPT
QPKTAPGIVVEALARRKSAGLSAFTVMSCDNMPENGHVMRNVILTYARALNSELARWIEQHVTFPSTMVDRIVPAVTHES
LDKITQLTGVSDPAAVACEPFRQWVIEDNFVAGRPGWEKAGAELVSNVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINE
CMADENYRLAAHSLMLHEQAPTLKVQGVDLGKYADSLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHNSEFD
LLALGVAAWMRYVGGVDEQGNAIEVSDPLLPLLQKAVAGSKEGEERVKALFAIEAIFGAELPKVTAFTAKVSAAYQLLLA
EGAKVAVAKYAAKLK

Sequences:

>Translated_495_residues
MELTGLLMENRLLNARATLPDYDRSKLVPRIVHLGFGAFHRAHQAVYTDILASEHGSDWGFHEVNLIGGEQQIADLKQQD
NLYTVAEMSADNWTARVVGVVKNALHAQVDGLESVLAVMCEPQVAIVSLTITEKGYCHSPATGELMLDHPMIVADLHNPT
QPKTAPGIVVEALARRKSAGLSAFTVMSCDNMPENGHVMRNVILTYARALNSELARWIEQHVTFPSTMVDRIVPAVTHES
LDKITQLTGVSDPAAVACEPFRQWVIEDNFVAGRPGWEKAGAELVSNVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINE
CMADENYRLAAHSLMLHEQAPTLKVQGVDLGKYADSLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHNSEFD
LLALGVAAWMRYVGGVDEQGNAIEVSDPLLPLLQKAVAGSKEGEERVKALFAIEAIFGAELPKVTAFTAKVSAAYQLLLA
EGAKVAVAKYAAKLK
>Mature_495_residues
MELTGLLMENRLLNARATLPDYDRSKLVPRIVHLGFGAFHRAHQAVYTDILASEHGSDWGFHEVNLIGGEQQIADLKQQD
NLYTVAEMSADNWTARVVGVVKNALHAQVDGLESVLAVMCEPQVAIVSLTITEKGYCHSPATGELMLDHPMIVADLHNPT
QPKTAPGIVVEALARRKSAGLSAFTVMSCDNMPENGHVMRNVILTYARALNSELARWIEQHVTFPSTMVDRIVPAVTHES
LDKITQLTGVSDPAAVACEPFRQWVIEDNFVAGRPGWEKAGAELVSNVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINE
CMADENYRLAAHSLMLHEQAPTLKVQGVDLGKYADSLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHNSEFD
LLALGVAAWMRYVGGVDEQGNAIEVSDPLLPLLQKAVAGSKEGEERVKALFAIEAIFGAELPKVTAFTAKVSAAYQLLLA
EGAKVAVAKYAAKLK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=486, Percent_Identity=79.4238683127572, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI1788497, Length=468, Percent_Identity=57.6923076923077, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1790779, Length=472, Percent_Identity=58.0508474576271, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1790028, Length=272, Percent_Identity=28.3088235294118, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI48994885, Length=389, Percent_Identity=24.9357326478149, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324401, Length=477, Percent_Identity=40.6708595387841, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6320765, Length=477, Percent_Identity=40.6708595387841, Blast_Score=350, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54405; Mature: 54405

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTGLLMENRLLNARATLPDYDRSKLVPRIVHLGFGAFHRAHQAVYTDILASEHGSDWG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FHEVNLIGGEQQIADLKQQDNLYTVAEMSADNWTARVVGVVKNALHAQVDGLESVLAVMC
CEEEEEECCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPQVAIVSLTITEKGYCHSPATGELMLDHPMIVADLHNPTQPKTAPGIVVEALARRKSAG
CCCEEEEEEEEECCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
LSAFTVMSCDNMPENGHVMRNVILTYARALNSELARWIEQHVTFPSTMVDRIVPAVTHES
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDKITQLTGVSDPAAVACEPFRQWVIEDNFVAGRPGWEKAGAELVSNVLPYEEMKLRMLN
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEEC
GSHSFLAYLGYLAGYQHINECMADENYRLAAHSLMLHEQAPTLKVQGVDLGKYADSLIAR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
YSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHNSEFDLLALGVAAWMRYVGGVDEQG
CCCCHHHCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
NAIEVSDPLLPLLQKAVAGSKEGEERVKALFAIEAIFGAELPKVTAFTAKVSAAYQLLLA
CEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
EGAKVAVAKYAAKLK
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MELTGLLMENRLLNARATLPDYDRSKLVPRIVHLGFGAFHRAHQAVYTDILASEHGSDWG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FHEVNLIGGEQQIADLKQQDNLYTVAEMSADNWTARVVGVVKNALHAQVDGLESVLAVMC
CEEEEEECCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPQVAIVSLTITEKGYCHSPATGELMLDHPMIVADLHNPTQPKTAPGIVVEALARRKSAG
CCCEEEEEEEEECCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
LSAFTVMSCDNMPENGHVMRNVILTYARALNSELARWIEQHVTFPSTMVDRIVPAVTHES
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDKITQLTGVSDPAAVACEPFRQWVIEDNFVAGRPGWEKAGAELVSNVLPYEEMKLRMLN
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEEC
GSHSFLAYLGYLAGYQHINECMADENYRLAAHSLMLHEQAPTLKVQGVDLGKYADSLIAR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
YSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHNSEFDLLALGVAAWMRYVGGVDEQG
CCCCHHHCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
NAIEVSDPLLPLLQKAVAGSKEGEERVKALFAIEAIFGAELPKVTAFTAKVSAAYQLLLA
CEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
EGAKVAVAKYAAKLK
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]