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The map label for this gene is pfkB [H]

Identifier: 218548708

GI number: 218548708

Start: 1379001

End: 1379933

Strand: Reverse

Name: pfkB [H]

Synonym: EFER_1345

Alternate gene names: 218548708

Gene position: 1379933-1379001 (Counterclockwise)

Preceding gene: 218548722

Following gene: 218548707

Centisome position: 30.07

GC content: 51.77

Gene sequence:

>933_bases
ATGGTACGTATCTACACGTTAACACTTGCGCCCTCACTCGACAGCGCGACAATTACCCCGCAGATTTATCCCGAAGGCAA
ACTACGCTGTTCTTCTCCCGTTTTTGAACCTGGTGGTGGCGGAATCAACGTCGCCCGAGCTATTGCTCATCTTAGCGGCA
GCGCGACAGCGATATTCCCGGCAGGCGGTGCTACGGGGGAGCATCTGGTCGCCCTGCTTGCCGATGAAAACGTCCCGGTC
GCCACCATCGAAACGAAAGACTGGACCCGACAAAATCTGCATGTCCATGTAGAATCCAGTGGCGAACAGTATCGTTTCGT
GATGCCTGGGGCCACGCTCAGTGAAGATGAGTTTCGTCAGCTCGAAGAGCAAGTCTTTGAAATAGAATCTGGTGCCATTC
TGGTCATAAGCGGCAGCCTGCCACCAGGAGTAGAACTGAAAAAGTTAACGCAACTTATTTCTGCCGCACAAAAGCAAGGG
ATTCGTTGCATTATTGATAGCTCTGGCGAAGCACTCGCAGCTGCGCTGGCAATTGGTCATATAGAGTTAGTAAAACCGAA
TCTCAAAGAATTGAGTGCCCTGGTTAATCGTGAGCTTTTACAACCCGATGACGTCCGCAAAGCAGCTCAGGAGATTGTCG
ACTGTGGTAAAGCCCATCGTGTAGTGGTGTCGCTTGGTCCACAAGGTGCCCTGGCAGTAGACAAGGAGTCATGCATTCAG
GTCGTTCCTCCTCCGGTAAAAAGTCAGAGTACAGTGGGTGCAGGTGACAGCATGGTGGGCGCAATGACACTGAAGCTTGC
AGAAGGAGCCTCTCTGGAAGATATGGTTCGATTTGGTGTAGCTGCCGGGAGTGCCGCAACACTCAATCAGGGAACACGTC
TATGTTCTGCTGACGACACTCACAAAATCTATGCCTGGCTTACCAGGCAATAA

Upstream 100 bases:

>100_bases
TTCTGATTGAAATAATAAATATCACAGAAATCATATTGTTAATTTCTTCCCTTTCTCGATATTCAGTGCCAGACTGAAAT
CAACCTGACAGGAGGAAAAG

Downstream 100 bases:

>100_bases
TTTCCGCTACGCGTTAAATCGTACAAACCTGTCGGTGAAGGGAATCACCGTCTATGCTCAAAACTCATGCGATTACAAAG
AGGTGAAAAAATGGCCAGCG

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSSPVFEPGGGGINVARAIAHLSGSATAIFPAGGATGEHLVALLADENVPV
ATIETKDWTRQNLHVHVESSGEQYRFVMPGATLSEDEFRQLEEQVFEIESGAILVISGSLPPGVELKKLTQLISAAQKQG
IRCIIDSSGEALAAALAIGHIELVKPNLKELSALVNRELLQPDDVRKAAQEIVDCGKAHRVVVSLGPQGALAVDKESCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAEGASLEDMVRFGVAAGSAATLNQGTRLCSADDTHKIYAWLTRQ

Sequences:

>Translated_310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSSPVFEPGGGGINVARAIAHLSGSATAIFPAGGATGEHLVALLADENVPV
ATIETKDWTRQNLHVHVESSGEQYRFVMPGATLSEDEFRQLEEQVFEIESGAILVISGSLPPGVELKKLTQLISAAQKQG
IRCIIDSSGEALAAALAIGHIELVKPNLKELSALVNRELLQPDDVRKAAQEIVDCGKAHRVVVSLGPQGALAVDKESCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAEGASLEDMVRFGVAAGSAATLNQGTRLCSADDTHKIYAWLTRQ
>Mature_310_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSSPVFEPGGGGINVARAIAHLSGSATAIFPAGGATGEHLVALLADENVPV
ATIETKDWTRQNLHVHVESSGEQYRFVMPGATLSEDEFRQLEEQVFEIESGAILVISGSLPPGVELKKLTQLISAAQKQG
IRCIIDSSGEALAAALAIGHIELVKPNLKELSALVNRELLQPDDVRKAAQEIVDCGKAHRVVVSLGPQGALAVDKESCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAEGASLEDMVRFGVAAGSAATLNQGTRLCSADDTHKIYAWLTRQ

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=309, Percent_Identity=90.6148867313916, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1788493, Length=276, Percent_Identity=31.8840579710145, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 32679; Mature: 32679

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSSPVFEPGGGGINVARAIAHLSGSATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
AGGATGEHLVALLADENVPVATIETKDWTRQNLHVHVESSGEQYRFVMPGATLSEDEFRQ
CCCCCHHHEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHH
LEEQVFEIESGAILVISGSLPPGVELKKLTQLISAAQKQGIRCIIDSSGEALAAALAIGH
HHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCE
IELVKPNLKELSALVNRELLQPDDVRKAAQEIVDCGKAHRVVVSLGPQGALAVDKESCIQ
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCE
VVPPPVKSQSTVGAGDSMVGAMTLKLAEGASLEDMVRFGVAAGSAATLNQGTRLCSADDT
ECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCEECCCCCC
HKIYAWLTRQ
CEEEEEEECC
>Mature Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCSSPVFEPGGGGINVARAIAHLSGSATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
AGGATGEHLVALLADENVPVATIETKDWTRQNLHVHVESSGEQYRFVMPGATLSEDEFRQ
CCCCCHHHEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHH
LEEQVFEIESGAILVISGSLPPGVELKKLTQLISAAQKQGIRCIIDSSGEALAAALAIGH
HHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCE
IELVKPNLKELSALVNRELLQPDDVRKAAQEIVDCGKAHRVVVSLGPQGALAVDKESCIQ
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCE
VVPPPVKSQSTVGAGDSMVGAMTLKLAEGASLEDMVRFGVAAGSAATLNQGTRLCSADDT
ECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCEECCCCCC
HKIYAWLTRQ
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]